EVALUATION OF INFLAMMATORY PROCESS LEVEL IN RATS AT EXPERIMENTAL CARCINOGENESIS AND EFFECT OF ENTESORPTION ON THEM

Authors

  • O. I. Kachur I. HORBACHEVSKY TERNOPIL STATE MEDICAL UNIVERSITY
  • L. S. Fira I. HORBACHEVSKY TERNOPIL STATE MEDICAL UNIVERSITY
  • P. H. Lykhatskyi I. HORBACHEVSKY TERNOPIL STATE MEDICAL UNIVERSITY

DOI:

https://doi.org/10.11603/mcch.2410-681X.2019.v.i2.10290

Keywords:

inflammatory processes, proinflammatory interleukins, anti-inflammatory interleukins, sorbent AUT

Abstract

Introduction. The problem of increasing malignant tumors of the colon is one of the most urgent in the field of medicine. The object of attention in the study of carcinogenesis of the large intestine is the state of the immune system of the organism and the activation of inflammatory processes in experimental animals. For the normalization of indicators of inflammation, methods of detoxification therapy are promising, namely enterosorption.

The aim of the study – to evaluate changes in the level of markers of inflammation in the blood of experimental animals with chemically induced carcinogenesis and the dynamics of their changes when introducing a carbon enterosorbent AUT.

Research Methods. The studies were conducted on white male rats. Animals were modeled colon cancer by administering 1,2=dimethylhydrazine hydrochloride at a dose of 7.2 mg/kg of body weight for 30 weeks. Enterosorbent AUT was injected intragastrally daily for 21 days after the carcinogenesis modeling in a dose of 1 ml of curd (corresponding to 0.2 g of pure weight of the drug) per 100 g of animal body weight. The activity of inflammatory processes was assessed by concentration of proinflammatory interleukin-6, interleukin 4 and C-reactive protein in the blood serum of experimental animals.

Results and Discussion. It was established that the administration of 1.2 dimethylhydrazine hydrochloride to animals is accompanied by a change in the cytokine profile and the content of C-reactive protein. There was an increase in pro-inflammatory interleukin 6, a decrease in anti-inflammatory interleukin 4 and an increase in the content of C-reactive protein in all terms of the study. The use of an enterosorbent AUT improved the results.

Conclusion. The obtained results confirm the positive dynamics of the use of detoxification therapy with sorbent AUT during the progressive development of inflammatory processes in conditions of simulated carcinogenesis.

References

Berezhnaya, N.M., & Chekhun, V.F (2005). Immu­nologiya zlokachestvennogo rosta [Immunology of ma­lignant growth]. Кyiv: Naukova dumka [in Russian].

Karmazina, I.S., & Kulinich, V.A. (2015). Kore­liatsiinyi analiz pokaznykiv bilkovoho obminu pry kan­tserohenezi ta pry zapalenni [Correlation analysis of protein metabolism indices at carcinogenesis and inflammation]. Pryrodnyi almanakh – Natural Almanac, 12, 112-118 [in Ukrainian].

Andreichyn, S.M., Lototska, S.V., & Meretskyi, V.M. (2015). Zminy pokaznykiv tsytokinovoi lanky imunitetu u khvorykh na KhOZL pry zastosuvanni enterosorbtsii [Changes in the indices of cytokine immunity in patients with COPD when using enterosorption]. Infektsiini khvoroby – Infectious Diseases, 3, 44-47 [in Ukrainian].

Nikolaev, V.G., Sakhno, L.A., & Snezhkova, E.A. (2015). Саrbon adsorbents: achievements and perspec­tives. Experimental Oncology, 1, 2-8

Gross, D., & Tolba, R. (2015). Ethics in aimal-based research. Eur. Surg. Res., 55 (1-2), 43-57. DOI: https://doi.org/10.1159/000377721

Gaber, W., Azkalany, G.S., Gheita, T.A., Mohey, A., & Sabry, R. (2013). Clinical significance of serum interleukin-6 and −174 G/C promoter polymorphism in Rheumatoid arthritis patients. Egypt. Rheumatol.,35 (2), 107-113. DOI: https://doi.org/10.1016/j.ejr.2012.11.002

Alybaeva, K.M, Berdyyarova, N.A, Mukhamed­zhanova, N.K, Maymakova, A.M., & Nurakhova, A.D. (2015). Analiz kolichestvennogo opredeleniya urovnya S-reaktyvnogo belka i prokaltsytonina u patsyyentov s infektsyonnoy patologiyey [Analysis of the quantitative determination of the level of C-reactive protein and procalcitonin in patients with infectious diseases]. Vestnyk AHYUV – Bulletin of AGIUV, 1-2, 36-40 [in Russian].

Okeh, U.(2009). Statistical problems in medical research. East. Afr. J. Public. Health, 6 (1), 1-7. DOI: https://doi.org/10.4314/eajph.v6i3.45762

Nikitin, Ye.V., Chaban, T.B., & Servetskyi, S.K. (2007). Suchasni uiavlennia pro systemu tsytokiniv [Contemporary notions about the system of cytokines]. Infektsiini khvoroby – Infectious Diseases 2, 64-69 [in Ukrainian].

Zhou, B., Shu, B., Yang, J., Liu, J., Xi, T., & Xing, Y. (2014). C-reactive protein, interleukin-6 and the risk of colorectal cancer: a meta-analysis. Cancer Causes Control, 25 (10), 1397-1405. DOI: https://doi.org/10.1007/s10552-014-0445-8

Vasyleva, G.I., Ivanova, I.A., & Tyukavkina, S.Yu. (2001). Tsytokiny – obshchaya sistema gomeostaticheskoy regulyatsii kletochnykh funktsyy [Cytokines – general system of homeostatic regulation of cellular functions]. Tsytologiya – Cytology, 43 (12), 1101-1111 [in Ukrainian].

Erlinger,T., Plaiz, E., Rifai, N., & Helzlsouer, K. (2004) C-reactive protein and the risk of incident colorectal cancer. JAMA, 5, 585-590. DOI: https://doi.org/10.1001/jama.291.5.585

Zhang, X., Liu, S., & Zhou, Y. (2016). Circulating levels of C-reactive protein, interleukin-6 and tumor necrosis factor-α and risk of colorectal adenomas: a meta-analysis. Oncotarget, 27, 7 (39), 64371-64379 DOI: https://doi.org/10.18632/oncotarget.11853

Godos, J., Biondi, A., Galvano, F., Basi­le, F., Sciacca, S., Giovannucci, E.L., & Grosso, G. (2017). Markers of systemic inflammation and colorectal ade­noma risk: Meta-analysis of observational studies. World J. Gastroenterol., 23 (10), 1909-1919. DOI: https://doi.org/10.3748/wjg.v23.i10.1909

Published

2019-07-10

How to Cite

Kachur, O. I., Fira, L. S., & Lykhatskyi, P. H. (2019). EVALUATION OF INFLAMMATORY PROCESS LEVEL IN RATS AT EXPERIMENTAL CARCINOGENESIS AND EFFECT OF ENTESORPTION ON THEM. Medical and Clinical Chemistry, (2), 24–29. https://doi.org/10.11603/mcch.2410-681X.2019.v.i2.10290

Issue

Section

ORIGINAL INVESTIGATIONS