THE TOTAL OXIDATIVE AND ANTIOXIDANT ACTIVITY IN THE ALLOTRANSPLANTED EMBRYONIC ABDOMINAL MUSCLE TISSUE IN RATS
Introduction. Allotransplantation of embryonic tissue is one of the most important directions in modern theoretical and medical biochemistry, which is being developed for stimulation and restoration of organism functions. Allotransplantation of embryonic tissue is considered possible alternative to traditional, conservative methods of treatment, as well as a methodological basis for experimental development.
The aim of the work – to investigate the effect of embryonic abdominal muscle tissue allotransplantation on the total oxidative and antioxidant activity.
Research Methods. Embryos aged 2–3 weeks, were used for embryonic muscle tissue allotransplantation. Under ethereal anesthesia under aseptic conditions, the animal was fixed to the surgical board in the position lying on the back; the operating area was shaved, and three times treated with an antiseptic. The embryos’ abdominal muscle tissue was removed and fixed with the ligature to the abdominal wall of the adult rat. The wound was tightly sutured in layers using interrupted stitch. The allotransplantation was performed according to the surgical rules of muscle surgery. The investigated indicators were measured on the first, third and seventh day after surgery in the tissue of the donor and the recipient. The transplantation of the formed (non-embryonic) tissue and the false operation were performed in order to eliminate the effect of the operation itself and the effect of tissue transplantation. That was done in order to detect the effect of embryonic tissue on the formed one. Therefore, we measured each indicator not only after transplantation of embryonic tissue, but also after the false operation and after transplantation of the formed tissue to the formed one and compared the same indicator for all three types of intervention.
Results and Discussion. The obtained results of general oxidant activity indicate that false surgery and allotransplantation of embryonic tissue have led to the development of oxidative stress in the muscle under investigation.
Conclusions. Thus, it was defined that the total oxidative activity is detected only on the seventh day of the investigation in all examination tissues both during the false operation and at allotransplantation of the embryonic muscle tissue. The total antioxidant activity is detected after the all performed surgical manipulations in both the donor and the recipient's tissue.
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