THE MORPHOLOGICAL CHANGES IN ORGANS OF RATS UNDER THE CONDITIONS OF COMPLEX PERSISTENT EFFECT OF HEAVY METAL SALTS AND PHOSPHORORGANIC PESTICIDES
Introduction. Lead poisoning is a major public health risk. Lead is an immunotoxicant, it has reproductive toxicity, liver toxicity. Lead and its compounds has many uses in industry for manufacturing paints, enamels, and others. The major hazard in industry arises from the inhalation of dust and fume of lead compounds.
The aim of the study – to research the effect of the lead acetate, the copper sulfate, the glyphosate (in herbicide Roundup) on morphological changes on the rat heart, kidneys, and liver tissue.
Research Methods. Experiments were performed on lab nonlinear white male rats of three age periods: puberty (youth, 70–90 g body weight and 2 to 3 months age); mature (average, weighing 170–210 g and aged 5–8 months) and aged rats processes in which catabolic processes prevail over anabolic (body weight 250–300 g and aged 20–24 months), which were injected intragastrically for 30 days with the lead acetate, the copper sulfate, the glyphosate (in herbicide Roundup) and correction for peptide. Rat tissue sections were prepared and observed under a light microscope for morphological changes. The study of histological preparations was performed after coloring with hematoxylin and eosin using a light microscope OLYMPUS CX 2.
Results and Discussion. We determined that introduction to animals of water solutions of lead acetate, copper sulfate and Roundup influences the functions of their organs. The morphological examinations revealed cellular lesions in the heart, kidneys, and liver tissue of rats caused by xenobiotics administration. The histological lesions included inflammation, hyperchromic anemia, polymorph infiltration of the glomeruli, and cellular degeneration kidneys, and liver. It was observed included symptoms of the hepatocytes’, necrosis and degeneration associated with infiltration in the liver of all experimental animals.
Conclusion. The effect of the lead acetate, the copper sulfate, the glyphosate (in herbicide Roundup) caused morphological changes of heart tissue, kidneys tissue, and liver tissue in rats.
Ahamed, M., & Siddiqui, M.K.J. (2007). Environmental lead toxicity and nutritional factors. Clin. Nutr., 26 (4), 400-408.
Jadhav, S.H., Sarkar, S.N., Patil, R.D., & Tripathi, H.C. (2007). Effects of subchronic exposure via drinking water to a mixture of eight water contaminating metals: A biochemical and histopathological study in male rats. Arch. Environ. Con. Toxicol., 53 (4), 667-677.
Vaziri, N.D., & Sica, D.A. (2004). Lead-induced hypertension: Role of oxidative stress. Curr. Hyper. Rep. J., 6, 314–320.
Baranowska-Bosiacka, I., Gutowska, I., Rybicka, M., Nowacki, P., & Chlubek, D. (2012). Neurotoxicity of lead. Hypothetical molecular mechanisms of synaptic function disorders. Neurol. Neurochir. Pol. J., 46 (6), 569-578.
Omobowale, T.O., Oyagbemi, A.A, & Olopade, J.O. (2014) Failure of recovery from lead induced hepatoxicity and disruption of erythrocyte antioxidant defence system in wistar rats. Environ. Toxicol. Pharm., 37 (3), 1202-1211.
Barros, R.C.H., Bonagamba, L.G.H., & Machado, B.H. (2002). Cardiovascular responses to chemoreflex activation with pottassium cyanide or hypoxic hypoxia in awake rats. Autonomic Neuroscience: Basic, and Clinical, 97, 110-115.
Zakon Ukrainy “Pro zakhyst tvaryn vid zhorstokoho povodzhennia” vid 21.02.2006 r., No 3447 [The Law of Ukraine "On the Protection of animals from ill-treatment" of 02.21. 006, No. 3447] [in Ukrainian].
Kozhemiakin, Yu.M., Khromova, O.S., & Filonenko, M.A. (2002). Naukovo-praktychni rekomendatsii z utrymannia laboratornykh tvaryn ta robota z nymy [Scientific and practical recommendations for the maintenance of laboratory animals and work with them]. Kyiv: Avitsena [in Ukrainian]
(1986). European convention for the protection of vertebrate animals used for experimental and other scientific purposes Council of Europe. Strasbourg.
Sapozhnikov, A.G., & Dorosevich, A.E. (2000). Gistolohgicheskaya i mikroskopicheskaya tekhnika: rukovodstvo [Histological and microscopic technique: a guide]. Smolensk: SAU [in Russian].