Analysis of effectiveness of oxygen insufflation, substance "kd-234" and reamberin on free radical oxidation processes in acute respiratory distress syndrome in rats

M. I. Marushchak, S. O. Savchuk, U. P. Gevko, R. S. Usinsky

Abstract


Introduction. In previous studies, we have demonstrated the development of oxidative stress in case of experimental acute respiratory distress syndrome (ARDS), which was characterized by a statistically significant increasing of active product soft hiobarbituric acid, dienic and triene conjugates concentration in liver tissues, decreasing of superoxide dismutase and catalase activity, and the content of SH-groups in animals with different resistance to hypoxia.

The aim of the study – to compare the effectiveness of the combination of oxygen insufflation, the substance "KD-234" and reamberin on oxidative stress level in rats with associated chest and both hip injuries.

Research Methods. Experimental studies were performed on 92 white nonlinear adult male rats aged 6–8 months. The animals were selected according to their individual resistance to hypoxia. Resistance of rats to acute hypoxia was carried out according to the method of V. Ya. Berezovskyi. Depending on the resistance to hypoxia, all animals were divided into medium-resistant (MRH) and low-resistant (LRH). We used G. Mutute-Bello experimental model. In the second test group for the correction we used the substance "KD-234", which was diluted in distilled water for injections and injected intragastric through a probe at a dose of 50 mg / kg, in the third study group – reamberin, at a dose of 10 ml / kg was intraperitoneally administered to animals one hour before the ARDS simulation.

Results and Discussion. The combination of oxygen insufflation with "KD-234" or reamberin had a significant positive effect on lipid peroxidation compared to oxygen insufflation, but the results were higher from the control level in animals with different resistance to hypoxia. The combined using of oxygen insufflation and "KD-234" substance in the experimental ARDS has a similar effect on the enzyme link of antioxidant protection, as in the case of oxygen insufflation, but it significantly increases the antioxidant-prooxidant index in the liver tissues, which is almost returns to the level of control in the groups of MRH- and LRH-animals.

Conclusion. In acute respiratory distress syndrome, the combination of oxygen insufflation and the substance "KD-234" due to antioxidant and antiradical properties.


Keywords


acute respiratory distress syndrome; correction; experiment.

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DOI: http://dx.doi.org/10.11603/mcch.2410-681X.2017.v0.i2.7975

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