Influence of carbon tetrachloride on the condition of free radical processes of rats’ organism and morphological changes in the colon

A. M. Pryshlyak, S. I. Yavorska, Т. К. Holovatа, B. Y. Reminetskyy


Introduction. Increased admission of herbal compounds in organism of humans and animals can trigger the development of functional disorders and pathological changes of the digestive system and in particular the colon.

The aim of the study – tо evaluate the state of free radical processes and morphological changes in the colon with toxic experimental animals exposed by carbon tetrachloride.

Research Methods. The study was performed on 44 white male rats weighing 180–200 g which were divided into 4 groups: group 1 – control (almost healthy animals); group 2–4 – rats with simulated toxic lesion by CCl4. Intragastric CCl4 was administered in 50.0 % oil solution at a rate of 0.2 ml of pure substance per100 g of body weight. The intensity of free radical processes evaluated with the level of malondialdehyde and diene conjugates, determined using spectrophotometric techniques; antioxidant system – with the activity of catalase in blood. According to the conventional method histological preparations were made. For qualitative and morphometric analysis system of a visual analysis of images using camera Vision Color CCD program and Inter Video Win DVR UTHSCSA Image Tool were used.

Results and Discussion. The results shows that animals carbon tetrachloride intoxication is accompanied by activation of free radical oxidation of lipids, as evidenced by the increase in blood serum of animal content as malondialdehyde (MDA) and diene conjugates (DC) throughout the experiment, with the maximum concentration observed in malondialdehyde 7 days after poisoning. Under the influence of CC14 catalase activity increases in serum at the second, seventh and fourteenth days of the experiment in 1.3, 1.2 and 2.4 times respectively. Morphometric techniques revealed a significant restructuring of the mucosa, submucosa, muscular layer and serous membrane of the colon, accompanied by severe vascular disorders of alterative, infiltrative processes and changes of morphometric parameters of the colon’s wall structures at tissue and organ levels.

Conclusions. In the case of CCl4 damage, the processes of free radical oxidation take place, the accumulation of toxic products of lipid peroxidation in the blood and the weakening of the enzyme system of antioxidant protection occur. A significant structural and morphometric reconstruction of the mucosa, submucosa, muscular and serous membranes of the colon has been established.


carbon tetrachloride; free radical oxidation; the wall of the colon.


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