Tableting mixtures containing immobilized lysozyme and quercetin: preparation, properties

I. I. Romanovska, S. S. Dekina, O. V. Sevastyanov, Ye. A. Rogozha

Abstract


Introduction. Lysozyme (EC 3.2.1.17), which has antibacterial, anti-inflammatory, immunomodulatory actions is increasingly used in medicine in a different dosage forms: tablets, wound coatings, capsules, gels, etc. Tablet is a convenient dosage form for usage of lysozyme in combination with other biologically active substances. The targeted stabilization of enzyme by immobilization into polymeric matrices determines the relevance of tablet form development of complex compositions of lysozyme and quercetin.

The aim of the study – development of tablet blends of complex preparation of immobilized lysozyme and quercetin, their quantitative determination and the study of the properties of active substances.

Methods of the research. Lysozyme activity was determined by bacteriolytic method. The protein content was controlled according to Lowry-Hartree, quercetin – using zirconium chloride.

Results and Discussion. Using the earlier obtained data about the interaction of lysozyme with polymeric supports of different origin and structure, for the development of tableting mixtures with lysozyme and quercetin, as polymeric binders the poly-N-vinylpyrrolidone, sodium salt of carboxymethyl cellulose and gelatin were chosen. The comparative analysis of physico-chemical properties of immobilized in polymers lysozyme had shown prospects of gelatin usage as a matrice, which promotes widening of pH-profile of enzyme activity both in areas of acidic and alkaline pH values, stability in acidic media. For making the lysozyme and quercetin – containing tableting mixtures, the fillers, disintegrants, flavours and wet granulation method were chosen. The total preservation of lysozyme activity and quercetin content in obtained granules was shown.

conclusions. Tablet mixtures with lysozyme and quercetin with usage of polymeric carriers of different origin and structure were developed. The analysis of biochemical and physico-chemical properties of immobilized lysozyme had shown prospects of gelatin usage as a matrice. Immobilization promotes widening of pH-profile of enzyme activity both in areas of acidic and alkaline pH values, stability in acidic media. 


Keywords


lysozyme; quercetin; immobilization; polymers; tablet mixtures.

References


Levytskyi, A.P. (2005). Lizotsym vmesto antibiotikov [Lysozyme instead antibiotics]. Odessa: Izd-vo KP OGT [in Russian].

Burbello, A.T., & Shabrov, A.V. Sovremennye lekarstvennye sredstva: Kliniko-farmakologicheskiy spravochnik prakticheskogo vracha [Modern medicines: Clinical and pharmacological reference book of a practical doctor]. Moscow: OlmaMediaGrupp/Prosveshchenie [in Russian].

Romanovska, I.I., Levytskyi, A.P., Dekina, S.S., & Ovsepian, A.M. (2015). Mukoadhezyvnyi hel z ímmobilizovanymy lízotsymom i kvertsetinom [Mucoadhesive gel with immobilized lysozyme and quercetin]. Míkrobiolohiia i biotekhnolohiia – Microbiology and Biotechnology, (3), 30-39 [in Ukrainian].

Chirumbolo, S. (2010). The role of quercetin, flavonols and flavones in modulating inflammatory cell function. Inflamm. Allergy Drug Targets, 9 (4), 263-285.

Hartree, E.F. (1972). Determination of protein: a modification of the Lowry method, that gives a linear photometric response. Analytical Biochemistry, 48 (2), 422-427.

Ogura, H., Shikiba, Y., & Yamazaki, Y. (1968). Quantitative analysis of flavonoids. J. of Pharmaceutical Sciences, 57 (4), 705-706.

Romanovska, I.I., Dekina, S.S., Psakhis, I.Y., & Psakhis, B.Y. (2016). Doslídzhennia chutlivosti mikroorhanizmiv do kombinatsiy lizotsimu z likarskymy rechovynamy [Investigation of the sensitivity of microorganisms to lysozyme combination with drugs]. Medychna khimiia – Medical Chemistry, 18 (2), 68-70 [in Ukrainian].

Dekina, S.S., Romanovskaya, I.I., & Gromovoy, T.Yu. Vliyaniye polimerov na protsessy assotsiatsii molekul lizotsima [The influence of polymers on the processes of association of lysozyme molecules]. Biopolymers and Cells, 27 (6), 442-445 [in Russian].

Dekina, S., Romanovska, I., Ovsepyan, A., Tkach, V., & Muratov, E. (2016). Gelatin/ carboxymethyl cellulose mucoadhesive films with lysozyme: Development and characterization. Carbohydrate Polymers, 147, 208-215.

Veys, A. (1971). Makromolekulyarnaya khimiya zhelatina [Macromolecular chemistry of gelatine]. Moscow: Izdatelstvo “Pishchevaya promyshlennost” [in Russian].




DOI: http://dx.doi.org/10.11603/mcch.2410-681X.2017.v0.i2.7966

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