Investigation of the effects of stevia and bilberry leaves extract on nhe performance of diabetes mellitus

A. L. Zagayko, O. I. Chumak


Introduction. Diabetes mellitus type 2 (DM 2) is a metabolic disease, which is characterized by chronic hyperglycemia. It develops as a result of insulin secretion disorder or mechanisms of its interaction with tissue cells. It is known that hyperglycemia is the main cause of cardiovascular complications development.

The aim of the work – to study the effect of dry extracts from stevia and bilberry leaves on the metabolic disorders development in rats with experimental diabetes mellitus, caused by dexamethozone on a high-fructose diet ground.

Methods of the research. The concentration of glycosylated hemoglobin, the level of fructosamine, the area under the glycemic curves, the level of immunoreactive insulin have been determined in the serum. Lipoperoxidation indicators have been determined according to diene conjugates (DC) and TBA-reactive products (TBA-RAP) content, the state of the antioxidant system has been assessed by reduced glutathione concentration determining (GSH) [8]. The significance of intergroup differences have been assessed due to Student's t-criterion.

Results and discussion The introduction of low-dose dexamethasone in laboratory animals causes a complex of disorders, which are specific for metabolic syndrome and type 2 diabetes. Thus, a rise in the level of glycosylated hemoglobin, a concentration of fructosamine, a glucose level, a basal glycemia level, an increase in the area under the glycemic curves are observed in the serum of rats under the indicated conditions. With the introduction of dry extracts of stevia and bilberry leaves together with dexamethasone, the normalizing influence of these substances on the investigated indicators is observed.

Conclusions. The use of dry extracts from stevia and bilberry leaves has a normalizing effect on glycosylation indicators, glucose level and lipid metabolism in the rats’ serum in type 2 diabetes mellitus. The more pronounced antioxidant effect of the extract from bilberry leaves and the hypoglycemic action of the extract from stevia leaves have been shown. This proves the advisability of the extracts from stevia and bilberry leaves joint application with the aim of drugs creation on their basis for metabolic disorders correction.


stevia leaf extract (Stevia rebaudiana Bertoni); bilberry leaf extract (Vaccinum myrtillus); type 2 diabetes; insulin resistance; dexamethasone.


Barchuk, O.Z., & Vronska, L.V. (2012). Vyznachennia vmistu BAR v ekstraktakh z lystia chornytsi zvychainoi [Determination of BAS content in extracts from blueberry leaves ordinary]. Pharmakolohichnyi chasopys – Pharmacological Journal, 1, 60-63 [in Ukrainian].

Zvorska, O., & Hroshovyi, T. (2009). Chornytsia zvychaina (Vaccinіum myrtillus L.) – perspektyvna rechovyna dlia oderzhannia likarskykh zasobiv [Blueberries usual (Vaccinіum myrtillus L.) – a promising substance for the obtaining of medicinal drugs]. Pharmakolohichnyi chasopys – Pharmacological Journal, 3, 29-33 [in Ukrainian].

Ilyin A.V., Arbuzova, M.I. & Knyazeva, A.P. (2008). Glikozilirovannyy gemoglobin kak klyuchevoy parametr pri minitoringe bolnykh sakharnym diabetom [Glycosylated hemoglobin as a key parameter in the monitoring of patients with diabetus mellitus]. Sakharnyy diabet – Diabetes Mellitus, 2, 60-64 [in Russian].

Ivashkin, V.T. (2010). Lipotkichnost i metabolichiskie narusheniya pri ozhyrenii [Lipotoxicity and metabolic disorders in obesity]. Gastroenterologiya, gepatologiya, koloprologiya – Gastroenterology, Hepatology, Coloprology, 2 (1), 4-13 [in Russian].

Ryazanova, T.K. (2013). Farmakologicheskoe issledovanie plodov i pobegov cherniki obyknovennoy [Pharmacological study of fruits and shoots of blueberry]. Fundamentalnye issledovaniya – Fundamental Research, 8 (5), 1136-1140 [in Russian].

Makarova M.N., & Makarov, V.G. (2004). Antiradikalnaya aktivnost flavonoidov I ikh kombinatsiy s antioksidantami [Antiradical activity of flavonoids and their combinations with antioxidants]. Farmatsiia – Pharmacy, 2, 30-32 [in Ukrainian].

Stroev, E.A. & Makarova, V.G. (1986). Praktikum po biologicheskoy khimii [Workshop on Biological Chemistry]. Moscow: Vyssh. shk. [in Russian].

Mikhalevskiy, O. (Ed.). (2005). Entsyklopedia narodnoy meditsiny [Encyclopedia of traditional medicine]. Lviv: Spolom [in Russian].

Artoni, S. Martin, C.K. & Han, H. (2010). Effects of stevia, aspartate, and sucrose on food intake, satiety, and postprandial glucose and insulin levels. Appetite, 55 (1), 37-43.

Baby Joseph, & D. Jini Rjmp (2011). In sight the hypoglycaemic effect of traditional indian herbs used the teratment of diabetes. IJPT, 42 (1), 170.

Buren, J. (1999). Dexamethasone decreases GLUT 1 and GLUT4 content in primary cultured rat adipocytes. Diabetol., 71 (2), 108-113.

Chen, T.H., Chen, S.C., & Chan, P. (2005). Mechanism of the hypoglycemic effects of stevioside, a glycoside of stevia rebaudiana. Planta Med., 53 (1), 73-76.

Gregersens S., Jeppesen, P.B., & Holst, J.J. (2004). Antihyperglycemic effects of stevioside in type 2 diabetic subjects. Metabolism, 53.

European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Strasbourg: Council of Europe, 8 (2) 109-116.

Salamar, M.R (2014). Relationships among insulin resistance, obesity, diagnosis of the metabolic syndrome and cardio-metabolic risk. Diabetes and Cardiovasc. Dis. Res., 2 (283), 367-373.

Severino, C., & Solinas, A. (2012). Low-dose dexamethosone in the rat: a model to study insulin resistance. Amer. J. of Physiol., 11 (9), 633-647.

Reddy, K.J. (2010). The role of the insulin resistance in the pathogenesis of atherosclerotic cardiovascular diseases: an uptated review. J. of Carviovasc. Med., 9, 633-647.



  • There are currently no refbacks.