ANALYTICAL ASPECTS OF THE INTERLABORATORY QUALITY CONTROL OF THE RESULTS OF ELEMENT ANALYSIS IN HUMAN BIOLOGICAL MEDIA
Introduction. The paper presents the analysis of the microelement composition of serum and the human whole blood as an important moment in clinical diagnostics. Standard samples of biological materials are a full-fledged carrier of correctness and precise measurement.
The aim of the study – to assess the correctness and precision of the determination of macro- (MaE) and microelements (ME) in the test serum and the whole blood by the method of multi-elemental analysis of optically-emission spectrometry with inductively coupled plasma (OES-ICP).
Research Methods. Samples of blood serum on the content of Na, K, Ca, Mg, Fe, Cu, Zn, P and those of the whole blood on the content of Pb, Cd, Mn, Se were prepared according to the validated and approved method of optical emission-spectrometry with inductively coupled plasma (OES-ICP) on the Optima 2100 DV by Perkin-Elmer (USA).
Results and Discussion. Studies on determination of 8 chemical elements in blood serum under the Sequas program (FORTRESS), conducted throughout a year, demonstrated a high number of good (6) and satisfactory (4) results of 12 measurements.The largest number of high coincidences in the LAMP program were established for obtaining adequate results, such as the use of a correction factor and types of spectral interference correction.The peculiarities of using the reference samples for interlaboratory tests and for the quality control of MaE and ME content in biological media are underlined. The perspectives of using modern spectral methods of multi-element analysis in clinical diagnostics of microelementosis were described.
Conclusions. The participation in the programs of interlaboratory comparisons makes it possible to improve own internal procedures for monitoring the quality of determination of macro- and microelements in biological media, provides for an additional external assessment of the testing abilities of laboratories and, thus, increases the reliability of the results obtained in laboratory diagnostics.
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