ANALYTICAL ASPECTS OF THE INTERLABORATORY QUALITY CONTROL OF THE RESULTS OF ELEMENT ANALYSIS IN HUMAN BIOLOGICAL MEDIA

  • I. N. Andrushyshyna YU. KUNDIIEV INSTITUTE OF OCCUPATIONAL HEALTH OF THE NAMS OF UKRAINE, KYIV
  • I. O. Golub YU. KUNDIIEV INSTITUTE OF OCCUPATIONAL HEALTH OF THE NAMS OF UKRAINE, KYIV
  • O. G. Lampeka ANALYTICAL ASPECTS OF THE INTERLABORATORY QUALITY CONTROL OF THE RESULTS OF ELEMENT ANALYSIS IN HUMAN BIOLOGICAL MEDIA
Keywords: macro- and microelements, standard samples of biological materials, reference values, interlaboratory quality control

Abstract

Introduction. The paper presents the analysis of the microelement composition of serum and the human whole blood as an important moment in clinical diagnostics. Standard samples of biological materials are a full-fledged carrier of correctness and precise measurement.

The aim of the study – to assess the correctness and precision of the determination of macro- (MaE) and microelements (ME) in the test serum and the whole blood by the method of multi-elemental analysis of optically-emission spectrometry with inductively coupled plasma (OES-ICP).

Research Methods. Samples of blood serum on the content of Na, K, Ca, Mg, Fe, Cu, Zn, P and those of the whole blood on the content of Pb, Cd, Mn, Se were prepared according to the validated and approved method of optical emission-spectrometry with inductively coupled plasma (OES-ICP) on the Optima 2100 DV by Perkin-Elmer (USA).

Results and Discussion. Studies on determination of 8 chemical elements in blood serum under the Sequas program (FORTRESS), conducted throughout a year, demonstrated a high number of good (6) and satisfactory (4) results of 12 measurements.The largest number of high coincidences in the LAMP program were established for obtaining adequate results, such as the use of a correction factor and types of spectral interference correction.The peculiarities of using the reference samples for interlaboratory tests and for the quality control of MaE and ME content in biological media are underlined. The perspectives of using modern spectral methods of multi-element analysis in clinical diagnostics of microelementosis were described.

Conclusions. The participation in the programs of interlaboratory comparisons makes it possible to improve own internal procedures for monitoring the quality of determination of macro- and microelements in biological media, provides for an additional external assessment of the testing abilities of laboratories and, thus, increases the reliability of the results obtained in laboratory diagnostics.

References

Oberlis, D., Kharland, B., & Skalnyy, A. (2008). Biologicheskaya rol makro- i mikroelementov u cheloveka i zhyvotnykh [The biological role of macro- and microelements in humans and animals]. Saint Petersburg: Nauka [in Russian].

Ivanenko, N.B., Solovyev, N.D., Ivanenko, A.A., & Moskvin, L.M. (2012). Opredeleniye khimicheskikh form mikroelementov v biologicheskikh obyektakh [Determination of chemical forms of microelements in biological objects]. Analitika i kontrol – Analytics and Control, 16 (2), 108-121 [in Russian].

Michalke, B. (2016) Metallomics: Analytical techniques and speciation methods. John Wiley & Sons.

Skalnyy, А.В., Lakarova, E.V., Kuznetsov, V.V., & Skalnaya, M.G. (2009). Analiticheskiye metody v bioelementologii [Analytical methods in bioelementology]. Saint Petersburg: Nauka [in Russian].

Makarenko, F.G., Voznesenskaya, T.V., & Menitskaya, V.I. (2001). Opredeleniye tyazhelykh metallov v nekotorykh organakh, tkanyakh I zhydkostyakh cheloveka v norme [Determination of heavy metals in certain organs, tissues and human fluids in the norm]. Sud. med. ekspertiza – Journal of Forensic Medical Examination, 5, 28 [in Russian].

Lisetskaya, L.G. (2005). Metodologicheskiye voprosy analiza mikroelementov v biosredakh [Methodological issues of analysis of trace elements in biological media]. Byul. VSNTs SO RAMN – Bulletin of the East Siberian Scientific Center of the Siberian Branch of the Russian Academy of Medical Sciences, 1 (39), 168-173 [in Russian].

Fedorov, V.I. (2006). Sovremennoye sostoyaniye problemy analiza neorganicheskikh elementov v syvorotke krovi [Current state of the problem of analysis of inorganic elements in blood serum]. Klin. lab. diagnostika – Clinical Laboratory Diagnostics, 4, 8-14 [in Russian].

Konovalova, S.O. (2002). Sravneniye informativnosti izucheniya razlichnykh biosubstratov dlya monitoring mineralnogo obmena [Comparison of the informativeness of studying various biosubstrates for monitoring mineral metabolism]. Ukr. biokhim. zhurn. – Ukrainian Biochemical Journal, 4 (4a), 145-146 [in Russian].

Andrusyshyna, І.M., Lampeka, O.H., Holub, I.O., Lubianova, I.P., & Kharchenko, T.D. (2014). Otsinka porushen mineralnoho obminu u profesiinykh kontynhentiv za dopomohoiu metodu atamno-emisiinoi spektrometrii z induktyvno zviazanoiu plazmoiu [Evaluation of mineral metabolism disorders in professional contingencies for the additional method of atomic emissive spectrometry from inductively connected plasma]. Kyiv: Avitsena [in Ukrainian].

Antomonov, M.Yu. (2006). Matematicheskaya obrabotka i analiz medico-biologicheskikh dannykh [Mathematical processing and analysis of biomedical data]. Kyiv: FMD [in Russian].

(2003). Vnutreniy kontrol kachestva rezultatov kolichestvennogo khimicheskogo analiza [Internal quality control of the results of quantitative chemical analysis]. Moscow: GOSSTANDART of Russia. Approved 10-01-03 [in Russian]

Protsenko, V.N. (2009). Osnovy obespecheniya kachestva klinicheskikh laboratornykh issledovaniy [Basics of quality assurance in clinical laboratory studies]. Kharkiv: IV [in Russian].

Published
2019-04-12
How to Cite
Andrushyshyna, I. N., Golub, I. O., & Lampeka, O. G. (2019). ANALYTICAL ASPECTS OF THE INTERLABORATORY QUALITY CONTROL OF THE RESULTS OF ELEMENT ANALYSIS IN HUMAN BIOLOGICAL MEDIA. Medical and Clinical Chemistry, (1), 10-16. https://doi.org/10.11603/mcch.2410-681X.2019.v0.i1.9993
Section
ORIGINAL INVESTIGATIONS