CHANGES OF BIOCHEMICAL INDEXES AND FUNCTIONAL ACTIVITY OF HEART OF HIGH AND LOW-RESISTANCETO ACUTE HYPOXIC HYPOXIA IN RATS OF DIFFERENT SEX IN IMMOBILIZATIONAL STRESS
Introduction. Determining the mechanisms of damaging the influence of stress on the heart in persons with different reactivity can contribute to the development of individual methods of correction.
The aim of the study–to determine the effect of immobilization stress on changes in peroxide lipid oxidation, antioxidant defense and ECG indices in rats of different sex with high and low resistance to hypoxia (HR, LR).
Research Methods. Stress was modeled 4 times by an hour immobilization of rats on a back down with an interval of 24 hours. In the heart, the diene and triaene conjugates, schiff bases, TBA-active products, superoxide dismutase (SOD), catalase activity were determined. ECG was recorded.
Results and Discussion. In control HR males, in comparison with LR, lower activity of lipid peroxidation (LPO), higher activity of SOD was observed; in HR-females, in comparison with LR, more products of LPH, SOD, less activity of catalase. Immobilization led to oxidative and carbonyl stress, more in males, compared to females. In the study of ECG only when stressed in HR-females increased the amplitude of the wave R, the deviation of ST relative to the isoline; In LR, the heart rate increased, the duration of the interval RR and QTc decreased.
Conclusions. Interval immobilization of rats leads to oxidative and carbonyl stress in the heart, but the mechanisms of their development depend on resistance to hypoxia and sex. With immobilization stress, changes in ECG are noted only in females.
Crea, F., Battipaglia, I., & Andreotti F. (2015). Sex differences in mechanisms, presentation and management of ischaemic heart disease. Atherosclerosis, 241 (1), 157-168.
Kötter, T., & Niebuhr, F. (2016). Resource-oriented coaching for reduction of examination-related stress in medical students: an exploratory randomized controlled trial. Adv. Med. Educ. Pract., 7, 497-504.
Rodin, R., Bonanno, G.A., Rahman, N., Kouri, N.A., Bryant, R.A., Marmar, C.R., & Brown, A.D. (2016). Expressive flexibility in combat veterans with posttraumatic stress disorder and depression. J. Affect. Disord., 207, 236-241.
Pimple, P., Shah, A.J., Rooks, C., Bremner, J.D., Nye, J., Ibeanu, I., Raggi P., and Vaccarino, V. (2015). Angina and mental stress-induced myocardial ischemia. J. Psychosom. Res., 78 (5), 433-437.
Berezovskyy, V.A. (1978). Gipoksiya i individualnye osobennosti reaktivnosti [Hypoxia and individual particularities of reactivity]. Kyiv: Naukova dumka [in Russian].
Kulinskiy, V.I., & Olkhovskiy, I.A. (1992). Dve adaptatsionnye strategii v neblagopriyatnikh usloviyakh: rezistentnaya i tolerantnaya. Rol gormonov i retseptorov [Two adaptation strategies in adverse conditions: resistant and tolerant. The role of hormones and receptors]. Uspekhi sovremennoy biologii – Advances of Modern Biology, 112, 697-711 [in Russian].
Khyshiktuyev, B.S., Khyshiktuyeva, N.A., & Ivanov, V.N. (1996). Metody opredeleniya produktov perekisnogo okisleniya lipidov v kondensate vydykhayemogo vozdukha i ikh klinicheskoe znachenie [Methods of determination of products of lipids peroxidation in exhaled air condensate and their clinical significance]. Klinicheskaya laboratornaya diagnostika – Clinical Laboratory Diagnostics, 3, 13-15 [in Russian].
Stefanov, O.V. (Ed.). (2001). Doklinichni doslidzhennia likarskykh zasobiv: metodychni rekomendatsii [Preclinical research of medicinal products: methodical recommendations]. Kyiv: Avitsenna [in Ukrainian].
Chevary, S., Chaba, I., & Sokei, Y. (1985). Rol superoksiddysmutazy v okislitelnykh protsessakh kletki i metod opredeleniya ee v biologicheskikh materialakh [The role of superoxide dismutase in the oxidative processes of the cell and the method for its determination in biological materials]. Laboratornoye delo – Laboratory Work, 11, 678-681 [in Russian].
Korolyuk, M.A., Ivanova. L.I., Mayorova, I.G., & Tokarev, V.E. (1988). Metod opredeleniya aktivnosti katalazy [Method for determination of catalase activity]. Laboratornoye delo – Laboratory Work, 1, 16-19 [in Russian].
Morozova, V.S. (2013). Funktsionuvannia antyoksydantnoi systemy miokardu shchuriv za umov shtuchnoho hipobiozu [Functioning of the antioxidant system of myocardium of rats under conditions of artificial gipobiosis]. Visnyk problem biolohii i medytsyny – Bulletin of Biological and Medical Problems, 2 (103), 3, 86-90 [in Ukrainian].
Copyright (c) 2018 Medical and Clinical Chemistry
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.