The study of inulin in artichoke inflorscences collected in Ukraine and in France
Keywords:artichoke, inulin, spectrophotometry, gas chromatography.
Introduction. There are over 12 000 medicinal plants encountered nowadays which are a biogenetically formed complex of biologically active compounds (BAC) and are extensively used in scientific as well as folk medicine. The plants which are used as food products and contain a large number of biologically active compounds are of great importance. Artichoke (Cynara scolymus L.) from Asteraceae family belongs to the group of plants which due to the presence of different groups of BAC is widely used as cholagogue, hepatoprotective, diuretic, antisclerotic, anti-inflammatory, analgesic, hypoglycaemic agent. BAC of artichoke is represented by carbohydrates, hydroxycinnamic acids, flavonoids, essential oil etc.
The aim of the study – identification and determination of the quantitative content of inulin in the artichoke inflorescences collected inUkraine andFrance.
Methods of the research. The presence of inulin in the studied plant material was confirmed using the Molisch’s test. The quantitative content of inulin in the artichoke inflorescences was determined spectrophotometrically (method 1) and by the means of GC chromato-mass-spectrometric method (method 2).
Results and Discussion. The appearance of the dark-violet coloring confirmed the presence of inulin in both samples of the plant material studied. The content of fructosanes’ sum, determined spectrophotometrically, was found to be 2.31 % in the artichoke inflorescences collected inUkraine, and 2.77 % in those collected inFrance. The content of inulin determined by the means of GC chromato-mass-spectrometry comprised 119.30 mg/ml and 147.60 mg/ml respectively.
Conclusions. The presence of inulin was confirmed using the Molisch’s test in both samples of artichoke inflorescences.
The content of inulin in the artichoke inflorescence collected inUkraineandFrancewas determined by the means of spectrophotometric and GC chromato-mass-spectrometric methods of analysis, and comprised 2.31% and 119.30 mg/ml respectively in the artichoke inflorescences collected inUkraine, and 2.77% and 147.60 mg/ml respectively in the artichoke inflorescences collected inFrance. The results obtained allow considering the plant material of artichoke of Ukrainian and French origin interchangeable for the biologically active substances obtaining.
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