Biochemical changes in rats’ periodont on the background of smoking

Authors

  • O. Ya. Lavrin I. HORBACHEVSKY TERNOPIL STATE MEDICAL UNIVERSITY
  • V. V. Shcherba I. HORBACHEVSKY TERNOPIL STATE MEDICAL UNIVERSITY
  • I. Ya. Krynytska I. HORBACHEVSKY TERNOPIL STATE MEDICAL UNIVERSITY

DOI:

https://doi.org/10.11603/mcch.2410-681X.2017.v0.i1.7682

Keywords:

periodont, smoking, oxidative stress.

Abstract

Introduction. In our days, smoking is one of the most important risk factors for inflammatory diseases. There is a lot of scientific data, devoted to research the effects of smoking on periodontal status. However, some aspects such as the status of free-radical oxidation, is left without proper attention.

The aim of the studyto investigate experimentally the indices of free radical oxidation in periodontal tissues on the background of smoking.

Methods of the research. Experimental studies were performed on 20 adult male albino rats weighing 160-180 g. Animals were divided into 2 groups: control (10 animals) and experimental (10 animals). Modeling of passive smoking was carried out by placing rats in a specially designed chamber in which tobacco smoke was distributed. The duration of the experiment was 21 days. The concentration of 8-isoprostane was determined by ELISA method using a set of "8-isoprostane ELISA» company «USBiological» (USA). Quantitative assessment of the content of metabolites of nitrogen (II) oxide was carried out by determining their amount. The content of reduced glutathione, superoxide dismutase and catalase activity in periodontal tissue homogenate were determined by conventional methods.

Results and Discussion. Concentration of 8-isoprostane in supernatant of periodontal homogenate in rats of control group was (3.94±0.18) pg/ml. In experimental animals this index was significantly increased in 2.9 times. This indicates that despite the action of tobacco smoke in periodontal tissues oxidative stress develops, characterized by the increasing intensity of the production of reactive oxygen species. On 22 day of the experiment, we have observed a significant decrease in superoxide dismutase activity in periodontal tissue homogenate by 40.9 % and catalase activity in 2.4 times compared to the control group. It is known that SH-groups (such as cysteine and methionine fragments of protein molecules) are most easily oxidized by reactive oxygen species to form a reversible and irreversible modifications. On 22 day of the experiment, we have observed a significant decrease in glutathione content in periodontal tissue homogenate by 24.4 % compared to the control group. The content of NO metabolites in supernatant of periodontal homogenate in rats of experimental group was significantly decreased in 2.1 times. The powerful factors that inactivate NO, are free radicals, among them – superoxide radical. Its interaction with NO leads to formation of peroxynitrite that as a strong oxidant has high degree of cytotoxicity.

Conclusions. In rats on the background of tobacco smoke in periodontal tissues significantly increases the generation of reactive oxygen species, reduces superoxide dismutase, and catalase activity and glutathione content, indicating a marked imbalance of free radical homeostasis. On the background of tobacco smoke in periodontal tissues occurs inhibition of nitroxydergic processes through significant reduction of nitric oxide metabolites content, which reduces the protective antibacterial potential.

Author Biography

O. Ya. Lavrin, I. HORBACHEVSKY TERNOPIL STATE MEDICAL UNIVERSITY

 

References

Kozar, V.V., Kudria, M.Ya. & Ustenko, N.V. (2010). Vyznachennia kontsentratsii metabolitiv oksydu azotu v syrovattsi krovi [Determination of metabolites of nitric oxide in blood serum]. Laboratorna diahnostyka – Laboratory Diagnostics, 3 (53), 14-16 [in Ukrainian].

Eshchenko, K.N., Zhadan A.V. & Shustval, N.F. (2013). Serdechno-sosudistaya sistema i kurenie [Cardiovascular system and smoking]. Liky Ukrainy – Medicines of Ukraine, 4, 12-17 [in Russian].

Kvasha, E.A. (2008). Znachimost faktorov riska dlia smertnosti muzhchin (prospektivnoe epidemiologicheskoe issledovanie) [Importance of risk factors for male mortality (prospective epidemiological study)]. Ukrainskyi kardiolohichnyi zhurnal – Ukrainian Journal of Cardiology, 2, 83-88 [in Russian].

Kontrol nad tiutiunom v Ukraini. Druhyi Natsionalnyi zvit. [Tobacco Control in Ukraine. Second National Report]. (2014). Kyiv: MOZ Ukrainy, DU «Ukrainskyi instytut stratehichnykh doslidzhen MOZ Ukrainy» [in Ukrainian].

Kochieva, I.V., Mkrtchian, S.N. & Khetagurov, S.K. (2015). Vliyanie tabakokureniya na mikrotsirkuliatciyu v tkaniakh paradonta [Influence of tobacco smoking on microcirculation in periodontal tissues]. Zhurnal nauchnykh statei «Zdorove i obrazovanie v XXI veke» – Journal of Scientific articles «Health and education in the XXI century», 17 (1), 57-58 [in Russian].

Krasovskyi, K.S. (2009). Naslidky hlobalnoi tiutiunovoi epidemii [The effects of the global tobacco epidemic]. SES. Profilaktychna medytsyna – SES. Preventive Medicine, 4, 72-74 [in Ukrainian].

Gerasimova, L.P., Al-Tabib, M.M., Kabirova, M.F., Usmanova, I.N. & Farkhutdinov, R.R. (2014). Lechenie vospalitelnykh zabolevanii parodonta u kuriashchikh lits molodogo vozrasta [Treatment of inflammatory periodontal diseases in smokers of young age]. Fundamentalnye issledovaniya – Basic Research, 7, 463-467 [in Russian].

Lizurchik, L.V. & Sheida, E.V. (2014). Vliyanie tabachnogo dyma na soderzhanie toksichnykh elementov v organizme krys [Effect of tobacco smoke on the content of toxic elements in the body of rats]. Vestnik OGU – Journal of the Orenburg State University, 6 (167), 71-74 [in Russian].

Koroliuk, M.A., Ivanova, L.I. & Mayorova, I.G. (1988). Metod opredeleniya aktivnosti katalazy [Method for the determination of catalase activity]. Laboratornoe delo – Laboratory Work, 1, 16-18 [in Russian].

Pikas, O.B. (2016). Kurinnia tsyharok sered naselennia ta yoho rol u rozvytku zakhvoriuvan [Smoking cigarettes among population and its role in development of diseases]. Visnyk problem biolohii i medytsyny – Journal of Medical and Biological Problems, 1, 1(126), 48-52 [in Ukrainian].

Vorobeva, E.N., Shumakher, G.I., Osipova, I.V., Khoreva, M.A. & Vorobev, R.I. (2006). Rol disfunktsii endoteliya v patogeneze ateroskleroza [The role of endothelial dysfunction in the pathogenesis of atherosclerosis]. Kardiovaskuliarnaya terapiya i profіlaktika – Cardiovascular Therapy and Prevention, 5 (6), 129-136 [in Russian].

Solomina, A.S. (2011). Vliyanie afobazola na geneticheskuyu i reproduktivnuyu toksichnost tabachnogo dyma u krys [The effect of afobazole on the genetic and reproductive toxicity of tobacco smoke in rats]. Candidate’s thesis. Moscow: NII farmakologii imeni V.V. Zakusova RAMN [in Russian].

Chevari, S., Chaba, I. & Sekei, I. (1985). Rol superoksidreduktazy v okislitelnykh protsessakh kletki i metod opredeleniya ee v biologicheskom materiale [The role of superoxide reductase in oxidative processes of the cell and the method of its determination in biological material]. Laboratornoe delo – Laboratory Work, 11, 678-681 [in Russian].

Ellman, G.L. (1959). Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics, 82, 70-77.

European convention for the protection of vertebrate animals used for experimental and other scientific purposes. (1986). Council of Europe. Strasbourg, 123, 52.

Hansen, R.E., Roth, D. & Winther, J. R. (2009). Quantifying the global cellular thiol-disulfide status. Proceedings of the National Academy of Sciences, 106, 2, 422-427.

Panjamurthy, K., Manoharan, S. & Ramachandran, C.R. (2005). Lipid peroxidation and antioxidant status in patients with periodontitis. Cellular and Molecular Biology Letters, 10 (2), 255-264.

Shirley, R., Ord, E.N.J. & Work, L.M. (2014). Oxidative Stress and the Use of Antioxidants in Stroke. Antioxidants, 3, 472-501.

Zhang, Z.-J. (2013). Systematic review on the association between F2-isoprostanes and cardiovascular disease. Annals of Clinical Biochemistry, 50, 108-114.

Published

2017-04-28

How to Cite

Lavrin, O. Y., Shcherba, V. V., & Krynytska, I. Y. (2017). Biochemical changes in rats’ periodont on the background of smoking. Medical and Clinical Chemistry, (1), 81–86. https://doi.org/10.11603/mcch.2410-681X.2017.v0.i1.7682

Issue

Section

ORIGINAL INVESTIGATIONS