Study of the influence of affinity purified antibodies against endocannabinoid receptor on cytokines content in the rats at experimental insulin resistance

  • A. L. Zahayko
  • T. O. Briukhanova
Keywords: metabolic syndrome, obesity, adiponectin, resistin, interleukins.

Abstract

The studies have revealed that prolonged intraperitoneal dexamethasone administration to rats against the fructose diet background is accompanied by the formation of insulin resistance syndrome. Pathological changes in cytokine (decrease of adiponectin, increase of resistin concentrations and IL‑6, α‑TNF as well) and hormonal (emergence hypercorticosteronemia) profiles of blood serum are observed under our experimental conditions. It was shown that a 3‑weeks administration of the studied drug (based on affinity purified antibodies against endocannabinoid receptor) substantially normalizes mentioned parameters. This is evidenced by the elevated levels of adiponectin, inhibition of resistin production and anti-inflammatory cytokines (IL‑6, α‑TNF), decrease of hypercorticosteronemia manifestation. The use of studied drug did not lead to changes of investigated parameters in the healthy animals, which have been keeping on a standard vivarium diet.

References

Gallagher, J., LeRoith, D., & Karnieli, E. (2011). The metabolic syndrome—from insulin resistance to obesity and diabetes Medical Clinics of North America., 95 (5), 855–873.

Dichi, I., & Simão, A. N. (2012) Metabolic syndrome: new targets for an old problem Expert opinion on therapeutic targets.,16 (2), 147–156.

Romeo, G. R., Lee, J., & Shoelson, S. E. (2012). Metabolic syndrome, insulin resistance, and roles of inflammation–mechanisms and therapeutic target Arteriosclerosis, thrombosis, and vascular biology. 32 (8), 1771–1776.

Illán-Gómez F. (2012) Obesity and inflammation: change in adiponectin, C-reactive protein, tumour necrosis factor-alpha and interleukin 6 after bariatric surgery Obesity surgery. 22 (6), 950–955.

Boiarynova, M. A., Rotar, O. P., & Konrady, A. O. (2014). Adypokyny y kardyometabolycheskyi syndrom Arteryalnaia hypertenzyia. 20 (5), 422–432.

Kheyfets, I. A. (2012). Experimental Study of the Effects of Dietressa, a New Weight-Reducing Drug Bulletin of experimental biology and medicine. 152 (3), 321–324.

Zahaiko, A. L., Briukhanova, T. O., & Shkapo. A. I. (2015). Modyfikatsiia metodu modeliuvannia eksperymentalnoi insulinorezystentnosti u shchuriv : informatsiinyi lyst K., 86, 7.

Lu, J. Y. (2008) Adiponectin: a biomarker of obesity-induced insulin resistance in adipose tissue and beyond Journal of biomedical science.15 (5), 565–576.

Ziemke, F., & Mantzoros, C. S. (2010). Adiponectin in insulin resistance: lessons from translational research The American journal of clinical nutrition. 91 (1), 258S–261S.

Sadashiv, S. T. (2012). Over expression of resistin in adipose tissue of the obese induces insulin resistance World journal of diabetes.,3, 7., 135–144.

Lukic, L. (2014). Hypertension in obese type 2 diabetes patients is associated with increases in insulin resistance and IL 6 cytokine levels: potential targets for an efficient preventive intervention International journal of environmental research and public health. 11 (4), 3586–3598.

Westerink, J., & Visseren, F. L. (2011). Pharmacological and non-pharmacological interventions to influence adipose tissue function Cardiovascular diabetology. 10 (1), 13–21.

Karpe, F., Dickmann, J. R., & Frayn, K. N. (2011). Fatty acids, obesity, and insulin resistance: time for a reevaluation Diabetes. 60 (10), 2441–2449.

Yvashkyn, V. T., & Maevskaia, M. V. (2010). Lypotoksychnost y metabolycheskye narushenyia pry ozhyrenyy Ros. zhurn. hastroэnterol., hepatol., koloproktol. 20 (1), 4–13.

la Fleur, S. E. (2006). The effects of glucocorticoids on feeding behavior in rats Physiology & behavior. 89 (1), 110–114.

Rodina, A. V., & Severin, S. E. (2012). The role of adiponectin in the pathogenesis of the metabolic syndrome and approach to therapy Patologicheskaia fiziologiia i eksperimentalnaia terapiia. 1, 15–25.

Published
2015-12-29
How to Cite
Zahayko, A. L., & Briukhanova, T. O. (2015). Study of the influence of affinity purified antibodies against endocannabinoid receptor on cytokines content in the rats at experimental insulin resistance. Medical and Clinical Chemistry, 17(4). https://doi.org/10.11603/mcch.2410-681X.2015.v17.i4.5682
Section
ORIGINAL INVESTIGATIONS