Experimental alimentary obesity: apoptosis, antioxidant system, macro- and microelements in the liver

  • M. I. Marushchak
  • O. P. Myalyuk
  • I. M. Klishch
Keywords: alimentary obesity, apoptosis, antioxidant protection, trace elements.

Abstract

The results of the study of the mechanism of liver injury of experimental alimentary obesity are provided. It was established that in 14 days the experiment activates superoxide dismutase and catalase, which are directly related to the level of zinc (r=0.77; p<0.05) and copper (r=0.64; p<0.05), which outside the active site of the enzyme Cu/Zn-SOD, providing regulation of the initiation stage peroxidation of chain oxidation. After 28 days of experiment functional capacity of antioxidant system is exhausted, as evidenced by decreased activity of SOD and catalase, the relationship with traсe elements is lost. Violation of trace elements metabolism after 28 days of experimental model in the rats are characterized by increased levels of copper, reduced zinc, magnesium and chromium in liver tissue, compare to the control (p<0.05). Also, apoptotic death of macrophages is present in the liver, which is 2.9 times higher than the control values, and depending on the level of zinc in liver tissue (p<0.05).

References

Boichuk, S. V., Shaimardanov, R. Sh., & Mynnebaev, M. M. (2007). Nekroz y apoptoz hepatotsytov y otsenka nekotorykh byokhymycheskykh parametrov krovy u bolnykh s mekhanycheskoi zheltukhoi opukholevoi etyolohyy, 6, 32–36.

Ghavami, S., Hashemi, M., & Kadkhoda, K. (2005). Apoptosis in liver diseases – detection and therapeutic applications. Med. Sci. Monit, 11, (11), 3337–3345.

Honchykova, S. Ch., & Ubeeva, Y. P. (2009). Dyzrehuliatsyia apoptoza hepatotsytov pry razlychnykh porazhenyiakh pecheny, vozmozhnosty fytokorrektsyy. Biulleten VSNTs SO RAMN, 2 (66), 236–238.

Kaletina, N. I., & Kaletin, G. I. (2007). Minor constituences – biological controls. М. Science in Russia, ed. Academy of Sciences, 1, 50.

Syzova, E. A., Tantsykuzhyna, A. A., & Poliakova, V. S. (2011). Ekspressyia markera apoptoza v kletkakh pecheny pry razlychnыkh sposobakh vvedenyia nanochastyts medy. Vestnyk OHU, 12 (131), 436–438.

Babenko, H. A., & Skalnyi, A. V. (2001). Spontannaia y yndutsyrovannaia khemyliumynestsentsyia syvorotky krovy pry khronycheskom alkoholyzme. Mykroelementy v medytsyne, 2 (2), 46–47.

Trak-Smayra, V., Paradis, V., & Massart, J. (2011). Pathology of the liver in obese and diabetic ob/ob and db/db mice fed a standard or high-calorie diet. Int. J. Exp. Pathol, 92 (6), 413–421.

Mialiuk, O. P., Klishch, I. M., & Marushchak, M. I. (2014). Zhovchoutvoriuvalna funktsiia pechinky za umovy eksperymentalnoho alimentarnoho ozhyrinniа. Visnyk problem biolohii i medytsyny, 4 (1), 164–167.

Marushchak, M. I., Antonyshyn, I. V., Mialiuk, O. P., Orel, Iu. M., & Krynytska, I. Ia. (2014). Sposib modeliuvannia alimentarnoho ozhyrinnia, № u 2013 12044, 3.

Chevary, S., Chaba, Y., & Sekei, I. (1985). Rol superoksydreduktazy v okyslytelnykh protsessakh kletky y metod opredelenyia ee v byolohycheskom materyale. Lab. delo, 11, 678–681.

Koroliuk, M. A., Yvanova, L. Y., & Maiorova, Y. H. (1988). Metod opredelenyia aktyvnosty katalazy. Lab. Delo, 1, 16–18.

Zhao, C., Tan, Y.C., & Wong, W.C. (2010). The CD14+/lowCD16+ monocyte subset is more susceptible to spontaneous and oxidant-induced apoptosis than the CD14+ CD16-subset. Cell Death and Disease, 1, 1–11.

García, O. P., Ronquillo, D., & María del Carmen Caamaño. (2012). Zinc, vitamin A, and vitamin C status are associated with leptin concentrations and obesity in Mexican women: results from a cross-sectional study. Nutrition &Metabolism, http://nutritionandmetabolism.biomedcentral.com/articles/10.1186/1743-7075-9-59.

Sörensen-Zender, I., Beneke, J.B., & Schmidt, M.W. (2013). Zinc-alpha2-glycoprotein in patients with acute and chronic kidney disease. BMC Nephrology, 14, 14.

Hromova, O. A., Fedotova, L. Э., & Hryshyna, T. R.(2014). Rol mahnyia v formyrovanyy metabolycheskoho syndroma, korrektsyy yzbytochnoho vesa y ozhyrenyia u detei y podrostkov. Pedyatryia, 2, 93, http://medi.ru/doc/a1501132.htm.

Gomes, M. R., Rogero, M.M., & Tirapegui, J. (2005). Considerations about chromium, insulin and physical exercise. Rev. Bras. Med. Esporte, 11(5), http://dx.doi.org/10.1590/S1517-86922005000500003

Boiechko, L. O., & Shmyhol, L. O. (2013). Osnovy molekuliarnoi biolohii (kurs lektsii). Cherkasy, Vyd. vid. ChNU imeni Bohdana Khmelnytskoho, 255.

Published
2015-12-29
How to Cite
Marushchak, M. I., Myalyuk, O. P., & Klishch, I. M. (2015). Experimental alimentary obesity: apoptosis, antioxidant system, macro- and microelements in the liver. Medical and Clinical Chemistry, 17(4). https://doi.org/10.11603/mcch.2410-681X.2015.v17.i4.5680
Section
ORIGINAL INVESTIGATIONS