THE EFFECT OF CELL THERAPY ON THE MANIFESTATIONS OF OXIDATIVE STRESS IN RATS OF DIFFERENT AGES UNDER CONDITIONS OF CRANIO-SKELETAL TRAUMA

Abstract

Introduction. Combined cranio-skeletal trauma (CST) is one of the most pressing problems of our time and occurs in people of all age groups. In the strategy of CST treatment in the post-shock period, the prevention of secondary brain and internal organ damage is at the forefront. Oxidative stress (OS) plays a leading role in its pathogenesis. In recent years, cell therapy has been actively studied to correct OS. The aim of the study. To determine the effect of cell therapy on the development of OS in rats of different ages under the conditions of experimental CST. Research Methods. The experiment was conducted on white male Wistar line rats of three age groups: immature at the age of 100–120 days, mature at the age of 6–8 months and old at the age of 19–23 months. Rats of each age group under thiopental sodium anaesthesia were modelled with CST. Rats of the control group were only put into general anaesthesia. For the purpose of correction, a suspension of cryopreserved neuroblast cells (NBC) was injected intravenously separately and in combination with injected intraperitoneally injection of mesenchymal stem cells (MSC). After 14 days of the experiment, catalase activity and the content of thiobarbituric acid reagents were determined in the liver, kidneys and lungs of different aged rats, and the ratio of these parameters was used to calculate the antioxidant-prooxidant index (API), the degree of decrease of which indicates the severity of OS. Results and Discussion. Under the influence of CST after 14 days of posttraumatic period in the liver, kidneys and lungs of rats, regardless of age, the value of API was statistically significantly lower compared to the control. After NBC monotherapy compared to rats without correction, an increase in API in internal organs was observed in experimental rats, but the result was statistically significant only in the liver of mature rats, and in the kidney and lungs of mature and immature rats. The use of a combination of NBC and MSC compared to rats without correction was accompanied by an increase in the value of API in all studied organs of immature and mature rats. Compared with the result of NBC monotherapy, the result was statistically significantly higher only in mature rats. Conclusions. There are age-related peculiarities of cell therapy effect on the development of OS in the internal organs of rats under conditions of CST. The efficacy of NBC monotherapy and combined NBC and MSC therapy is significantly higher in mature rats compared to rats of other age groups.