INVESTIGATION OF POLYPHENOLS CONTENT IN THE PHYTOSUBSTANCES FROM NEW ENGLAND ASTER HERB OBTAINED BY MACERATION WITH STIRRING

Abstract

Introduction. Aster novae-angliae L. is a perennial herbaceous plant native to North America. This species has been traditionally used for the treatment of respiratory diseases, including cough, asthma, and colds. New England aster contains a complex of biologically active substances with antioxidant, anti-inflammatory, and antimicrobial properties. The aim of the study – to determine the quantitative content of the total polyphenols in phytosubstances from the herb of Aster novae-angliae of the Andenken an Alma Pötschke variety, obtained by maceration with stirring. Research Methods. The objects of the study were phytosubstances from the herb of Aster novae-angliae L., obtained by maceration with stirring in a raw material-to-extractant ratio of 1:10, using 10% ethanol as the extractant (series 1) and 20% ethanol (series 2). The quantitative content of the total polyphenols, recalculated as pyrogallol, was determined using the spectrophotometric method. Results and Discussion. The results of the study showed that the quantitative content of total polyphenols in the phytosubstance obtained from the herb of Aster novae-angliae of the Andenken an Alma Pötschke variety using 10% ethanol was 5,62±0,22%. The content of total polyphenols in the phytosubstance obtained using 20% ethanol from the studied raw material was 6,29±0,10%. The conducted research established that the quantitative content of total polyphenols in the phytosubstance obtained using 20% ethanol from the herb of Aster novaeangliae was 1,12 times higher than in the extract obtained using 10% ethanol. Conclusions. The quantitative content of total polyphenols in phytosubstances from the herb of Aster novaeangliae of the Andenken an Alma Pötschke variety, obtained by maceration with stirring in a raw material-toextractant ratio of 1:10, was determined using 10 and 20% ethanol as extractants. It was established that the use of 20% ethanol extracted slightly more total polyphenols, with their quantitative content amounting to 6,29±0,10%. In the phytosubstance obtained using 10% ethanol, the total polyphenol content was 5,62±0,22%. The obtained results can serve as a basis for microbiological and pharmacological studies and can be used in the development of dietary supplements or medicinal products based on Aster novae-angliae L.