INVESTIGATION OF THE AMINO ACID COMPOSITION OF THE RAW SORREL BY THE METHOD OF GAS CHROMATO-MASS SPECTROMETRY

Abstract

Introduction. Sorrel (Rumex patientia L. × Rumex tianshanicus Losinsk.) is a valuable gene pool of a new culture, an interspecies hybrid of spinach sorrel with Tian Shan sorrel, which was obtained as a result of breeding work in the 90s of the 20th century. scientists of the department of cultural flora of the National Botanical Garden named after M. M. Hryshko of the National Academy of Sciences of Ukraine (Kyiv). It is a valuable fodder, vegetable, food, medicinal plant. It is also important as a bioenergy plant. Considering the fact that sorrel has not been studied so far, its phytochemical research is relevant.

The aim of the research – to establish and determine the quantitative content of individual amino acids in the raw material of the studied plant.

Research Methods. The material for the research was the stems, flowers, leaves, roots and seeds of sorrel of the Kyiv Ultra variety, which were collected during the flowering of plants in 2022 at the research plots of the cultural flora department of the National Botanical Garden named after M. M. Hryshka of the National Academy of Sciences of Ukraine (Kyiv). The qualitative composition and quantitative content of amino acids were determined on an Agilent 6890N/5973inert (Agilent technologies, USA) gas chromatography-mass spectrometric system.

Results and Discussion. The research results showed that 14 bound and 13 free amino acids were identified in sorrel leaves and seeds, 16 bound and 11 free in roots, 11 bound and 7 free in stems, and 12 bound in inflorescences. bound and 5 free. The highest total content of free amino acids was found in the seeds of the plant (29.69 mg/g), somewhat lower – in sorrel leaves (24.93 mg/g) and the lowest – in stems (1.06 mg/g). A significant amount of free amino acids L-proline was found in all studied objects, except for stems. The highest total content of bound amino acids was found in sorrel leaves (89.55 mg/g), somewhat lower – in seeds and inflorescences (70.03 mg/g and 62.96 mg/g, respectively). An insignificant total amount of bound amino acids was found in sorrel roots and stems (29.33 and 7.44 mg/g, respectively).

Conclusions. The qualitative composition and quantitative content of free and bound oxalate amino acids of leaves, seeds, roots, stems and inflorescences was determined. 14 bound and 13 free amino acids were found in sorrel leaves and seeds, 16 bound and 11 free in roots, 11 bound and 7 free in stems, 12 bound and 5 free in inflorescences. Among free amino acids, phenylalanine and L-valine dominated in leaves and seeds, respectively, in roots – L-lysine and L-valine, in inflorescences – L-aspartic acid, in stems – glycine. Of the bound amino acids, L-leucine, L-proline, and L-aspartic acid predominated in oxalic acid leaves, L-leucine and L-aspartic acid in seeds, L-phenylalanine and L-tryptophan in roots, and L-tryptophan in stems – L-aspartic acid and L-proline, in inflorescences – L-leucine and L-phenylalanine.