THE INFLUENCE OF A MULTIVITAMIN COMPLEX ON THE STATE OF PANCREAS OF HAMSTERS UNDER CONDITIONS OF EXPERIMENTAL METABOLIC SYNDROME
DOI:
https://doi.org/10.11603/mcch.2410-681X.2023.i3.14132Keywords:
experimental metabolic syndrome, pancreas, multivitamin complex (MVC), hamstersAbstract
Introduction. Under the conditions of metabolic syndrome (MS), there is a violation of the pancreas function, therefore, the pancreaprotective properties of drugs used for the treatment of MS are important.
The aim of the study – to investigate the effect of a multivitamin complex (MVC) (Aevit premium produced by JSC “Kyiv Vitamin Plant”) on the structure of the pancreas tissue of Syrian golden hamsters under the conditions of experimental MS induced by a cafe diet.
Research Methods. MS was caused by a cafe diet with a fat content 40 %. The prepared mixture was given to animals for 7 weeks (49 days). Drinking water was replaced with 10 % fructose solution. The studied MVC (25.8 mg/kg) and comparator drugs: metformin (60.0 mg/kg) and vitamin E (100 mg/kg) were applied starting from the 5th week of MS during 3 weeks (21 days). Pancreas micropreparations were prepared according to generally accepted histological methods. The microscopic preparations were viewed under a Granum L 30 (03) light microscope, microscopic images were photographed with a Granum DSM 310 digital video camera. The photographs were processed on a Pentium 2.4 GHz computer using the Toup View program.
Results and Discussion. After consumption of a cafe diet for 7 weeks in the hamsters pancreas, certain signs of the insular apparatus exhaustion were established: a decrease in the “quality” of beta cells, a decrease of cells amount; an increase in the relative proportion of very small and small and a decrease in the proportion of medium pancreatic islets. Under the influence of MVC, the number of pancreatic islets, their percentage distribution by classes significantly exceeded similar indicators in animals with control pathology and practically corresponded to the intact control.
Conclusions. The investigated MVC contributed to the reduction of the stress of insulinocytes and pancreatocytes of the pancreas in Syrian hamsters with a model of metabolic syndrome. It was established that in terms of the expressiveness of the corrective effect on the condition of the pancreas of hamsters under the conditions of MC, PVC is not inferior to vitamin E and metformin.
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