RESEARCH ON THE DEVELOPMENT OF THE PRODUCTION TECHNOLOGY AND DETERMINATION OF THE QUANTITATIVE CONTENT OF BIOLOGICALLY ACTIVE SUBSTANCES IN THE MOUTH RINSE

Abstract

Introduction. Individual oral hygiene is an important element of general human hygiene, as insufficient hygiene can lead to the development of such dental diseases as gingivitis, caries, periodontitis, as well as bad breath (halitosis). Some studies show that 25 to 50 % of the world's population can suffer from halitosis. Halitosis can be a temporary or chronic condition, and its occurrence depends on many factors – such as health, diet, lifestyle, oral hygiene, etc.

The aim of the study – to conduct experimental research on the development of a technology for the production of a mouth rinse with a complex effect for use in halitosis and methods for determining the quantitative content of active pharmaceutical ingredients in it.

Research Methods. The objects of the study were model samples of a mouth rinse, an Evolution 60s spectrophotometer (Thermo Fisher Scientific, USA), "AXIS" analytical balances (Poland), class A measuring vessels, and reagents that meet the requirements of the State Pharmacopoeia of Ukraine (SPU).

Results and Discussion. Based on the previously conducted physico-chemical and pharmacotechnological studies, a rational technology was developed, and a basic technological scheme of a therapeutic and prophylactic oral rinse was drawn up. Quantitative assessment of the amount of flavonoids in terms of hyperoside and isoquercitroside was carried out by the sum of substances of the flavonoid structure using a spectrophotometric method based on the complexation reaction of aglycones with aluminum salts after preliminary hydrolysis of the compounds. The maximum of the colored reaction products was observed at a wavelength of 425 nm, the content of the sum of flavonoids was calculated by the method of the specific absorption index, which is 500. The precision of the experimental results was characterized by a low standard deviation in the studied concentration range (RSD=0.16 %), the systematic error is about 0.09 %, the correlation coefficient of the proposed method r=0.9999. Determination of polysaccharides should be carried out by the sum of the substances of the polysaccharide structure by the method of gravimetry. The method is precise, as the standard deviation in the studied concentration range (RSD=0.11 %), correct – systematic error at the level of 0.01 %, and linear – the correlation coefficient of the proposed method is r=0.9984.

Conclusions. A rational technology of a therapeutic and preventive oral rinse and a basic technological scheme of its production have been developed, and the critical parameters of this process have been determined. Methods for quantitative determination of the amount of polysaccharides (at least 10.0 g per 100.0 ml of solution) and the amount of flavonoids (at least 45.0 mg per 100 ml of solution) have been developed.