INVESTIGATION OF COUMARINS OF ANGELICA BY THE METHOD OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

Introduction. The genus Angelica or Piper (Angelica L.) from the celery family (Apiaceae), includes about 100 species of biennial or perennial herbaceous plants, widely distributed in Asia, Europe and North America. Archangelica archangelica (Angelica archangelica L.; Archangelica officinalis (Moench.) Hoffm.) and forest angelica (Angelica sylvestris) grow in Ukraine. Biologically active substances of angelica have antispasmodic, expectorant, diuretic, sedative, diaphoretic, choleretic effects. Previous studies have shown that angelica contains organic, amino and fatty acids, carbohydrates, hydroxycinnamic acids, tannins, flavonoids, and volatile compounds. In the sources of scientific literature, there is not enough information on the study of coumarins of this species.

The aim of the study – to determine the coumarins in the leaves, stems and rhizomes and roots of angelica by the method of high-performance liquid chromatography (HPLC).

Research Methods. The qualitative composition and quantitative content of coumarins in the studied objects were determined by HPLC on an Agilent 1200 3D LC System Technologies (USA) chromatograph.

Results and Discussion. As a result of HPLC analysis, the content of simple coumarins – esculetin and umbelliferone and furocoumarins – isopimpineline and bergapten was identified and quantified in the investigated objects of angelica. HPLC analysis showed that angelica medicinal rhizome and roots contain a significant content of bergapten – 957.50 μg/g. The leaves and stems of the studied species contained a much lower amount of coumarin compounds. No esculetin was detected in the stems, the content of which was 38.99 μg/g in the leaves, and no isopimpinelin was detected in the leaves either.

Conclusions. The quantitative content of simple coumarins – esculetin and umbelliferone and furocoumarins – isopimpinelin and bergapten was identified and determined using the HPLC method in the rhizomes and roots, leaves and stems of angelica. It was established that the medicinal rhizome and roots of angelica contain a significant content of bergapten, amounting to 957.50 μg/g. The obtained data indicate that angelica medicinal rhizomes and roots are promising medicinal plant raw materials and require further research.