CHANGES IN THE PARAMETERS OF MINERAL METABOLISM IN THE BLOOD OF RATS WITH LIPOPOLYSACCHARIDE-INDUCED PERIODONTITIS COMBINED WITH CHRONIC THIOLACTONE HYPERHOMOCYSTEINEMIA
DOI:
https://doi.org/10.11603/mcch.2410-681X.2023.i1.13739Keywords:
periodontitis, hyperhomocysteinemia, total calcium, inorganic phosphate, ratsAbstract
Introduction. Generalized periodontitis (GP) is still one of the most pressing problems in dentistry. The development of destructive changes in the periodontal complex in GP is due to the level of metabolic disturbance in its organic and mineral components. On the other hand, comorbid general somatic pathologies make a significant difference in the etiopathogenesis of GP, in particular, one of these pathologies that can complicate the course of GP is syndrome of hyperhomocysteinemia (HHcy), characterized by an increased content of the amino acid homocysteine (Hcy) in the blood.
The aim of the study – to investigate the changes in the parameters of mineral metabolism in the blood of rats with lipopolysaccharide (LPS)-induced periodontitis against the background of chronic thiolactone HHcy.
Research Methods. The experiments were performed on 48 mature inbred white rats, which were divided into the following groups: I – control group (n=12); II – animals with LPS-induced periodontitis (n=12); III – animals with chronic thiolactone HHcy (n=12); IV – animals with LPS-induced periodontitis combined with HHcy (n=12). The content of total Hcy by enzyme immunoassay using the kit of the firm "Axis-Shield" (Great Britain). Determination of the content of total calcium and inorganic phosphate in blood serum was carried out on a semi-automatic biochemical analyzer Humalyzer 2000 (Human, Germany).
Results and Discussion. LPS-induced inflammation of the periodontium in rats is accompanied by a violation of calcium-phosphorus metabolism, which is manifested by an increase in the content of total calcium in the blood serum by 10.9 % (p=0.035). Chronic thiolactone hyperhomocysteinemia enhances mineral imbalance in LPS-induced periodontitis, which is confirmed not only by more pronounced hypercalcemia in animals with LPS-induced periodontitis without comorbid pathology, but also by hyperphosphatemia and the presence of probable associations between the level of homocysteine and total calcium in blood serum.
Conclusions. More pronounced disorders of mineral metabolism in rats with LPS-induced periodontitis against the background of chronic hyperhomocysteinemia compared to rats with LPS-induced periodontitis without comorbid pathology are probably associated with the osteotoxic effect of high levels of homocysteine, namely, activation of osteoclastogenesis, increased bone resorption and specific accumulation of homocysteine in bone tissue.
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