INDICATORS OF ENDOGENOUS INTOXICATION IN RATS UNDER CHRONIC ALCOHOL INTOXICATION

Abstract

Introduction. Alcoholism is an important medical and social problem, which often leads to disability and mortality of the population. Many systems and organs are affected by the toxic effect of alcohol, because it easily interacts with many vital processes of the body. Due to the syndrome of endogenous intoxication, we can evaluate a lot of data and get results about the course of many diseases at the level of molecules of average mass and changes in the erythrocyte index

The aim of the study – to investigate the changes in indicators of endogenous intoxication at the level of mo­lecules of average mass and changes in the erythrocyte index in rats with chronic ethanol intoxication.

Research Methods. The experiments were carried out on 48 sexually mature rats – 24 males and 24 females, which were divided into two groups: the first group served as a control, the animals of the second group were intragastrically injected with a 30 % ethanol solution at the rate of 2 ml per 100 g of body weight once for 28 days. A month after the start of the study, experimental animals were euthanized by bloodletting under thiopental anesthesia. The degree of expression of endogenous intoxication was assessed by the content of medium-mass molecules, circulating immune complexes, and the erythrocyte intoxication index in the blood serum. The obtained results were analyzed statistically.

Results and Discussion. As a result of the study, a pronounced increase in endotoxicosis indicators was observed in individuals of both sexes, but it was more significant in females. In particular, the index of erythrocyte intoxication in males increased by 1.42 times, and in females by 1.61 times. The level of the content of molecules of average mass in males increased by 2.25 times, and in females by 2.29 times. Compared to the control group of animals, the growth of circulating immune complexes in males was 1.17 times, and in females – 1.33 times.

Conclusions. The results of the research show that in animals of both sexes, under the conditions of chronic ethanol exposure, there is a gradual increase in indicators of endogenous intoxication – molecules of average mass, erythrocyte index of intoxication and circulating immune complexes, and the indicators of growth in females are more significant than in males. This indicates the negative impact of chronic ethanol intoxication on the state of membrane structures, production and elimination of endogenous toxicants, which can cause dysfunction of many organs and body systems.