THE SIGNIFICANCE OF OXIDATIVE PROCESSES DURING BLOOD LOSS IN THE HEART OF RATS OF DIFFERENT SEXES

Authors

  • I. Yu. Bevzyuk I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • V. A. Miroshnyk I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • S. V. Chornij I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • O. V. Denefil I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • R. S. Usynsky I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • T. Y. Yaroshenko I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY

DOI:

https://doi.org/10.11603/mcch.2410-681X.2022.i3.13381

Keywords:

lipid peroxidation, antioxidant system, heart, blood loss, rats of different sexes

Abstract

Introduction. In recent months, in connection with the war on the territory of Ukraine, the number of people with injuries and, as a result, blood loss has increased significantly. Studying the mechanisms of damage to internal organs in the early and late periods after blood loss is an urgent issue. The leading link in the development of pathology remains the development of oxidative stress, which affects the development of cardiovascular pathology.

The aim of the study – to evaluate the development of oxidative stress in the heart homogenate of rats of different sexes in different periods after blood loss.

Research Methods. Experiments were performed on 70 outbred rats of different sexes weighing 190-230 grams. Animals were divided into 2 groups – control, blood loss. Blood loss was simulated under sodium thiopental anesthesia by cutting the femoral vein (20 % of the volume of circulating blood). Slaughter of animals was carried out in control, 1, 7, 14 and 28 days after blood loss. A heart sample was taken, in the homogenate of which diene conjugates (DC), TBA-active products (TBA-ap), superoxide dismutase (SOD) and catalase activity (Cat) were determined. A morphological study of the myocardium was carried out in Heidenhain-stained preparations.

Results and Discussion. In control male rats, higher values of DC and TBA-ap and lower indicators of SOD and Cat activity were noted. The activation of lipid peroxidation (LPO) processes was noted in the dynamics of observation of rats after blood loss, which was most pronounced after 1 day. In males, LPO products prevailed throughout the experiment. Antioxidant activity was also lowest after 1 day, and recovery was noted in females after 14 days, and in males after 28.

Conclusion. The development of oxidative stress in the heart of rats with blood loss depends on the initial activity of the antioxidant system and sex. More pronounced damaging changes were noted in males. Greater activity of antioxidants prevents significant damage to the myocardium.

References

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Published

2022-12-16

How to Cite

Bevzyuk, I. Y., Miroshnyk, V. A., Chornij, S. V., Denefil, O. V., Usynsky, R. S., & Yaroshenko, T. Y. (2022). THE SIGNIFICANCE OF OXIDATIVE PROCESSES DURING BLOOD LOSS IN THE HEART OF RATS OF DIFFERENT SEXES. Medical and Clinical Chemistry, (3), 76–80. https://doi.org/10.11603/mcch.2410-681X.2022.i3.13381

Issue

No. 3 (2022)

Section

ORIGINAL INVESTIGATIONS

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