STUDY OF TANNINS IN THE RAW MATERIALS OF ANGELICA SYLVESTRIS L. AND ANGELICA ARCHANGELICA L. BY THE HIGH PERFORMANCE LIQUID CHROMATODRAPHY

Abstract

Introduction. The search for new promising types of medicinal plants is relevant at the current stage of the development of pharmaceutical science. One of the little-studied species are representatives of the celery family (Apiaceae) of the genus Angelica (Angelica L.), angelica (Angelica archangelica L.) and angelica (Angelica sylvest­ris L.). These species are used in folk medicine as an expectorant, carminative, diaphoretic and diuretic. No information on the comprehensive study of these species of the Angelica genus was found in scientific publications.

The aim of the study – to determine the tannins in the leaves and rhizomes and roots of Angelica archangelica L. and Angelica sylvestris L.by the method of high-performance liquid chromatography (HPLC).

Research Methods. The objects of study were the leaves and rhizomes and roots of the Angelica archange­lica L. and the Angelica sylvestris L., which were collected in the territory of the Ternopil region. Leaves were collected during mass flowering of plants, underground organs – after the death of the above-ground part.

The qualitative composition and quantitative content of tannin components in the studied objects were determined by HPLC on an Agilent 1200 3D LC System Technologies (USA) chromatograph.

Results and Discussion. As a result of the research conducted in the leaves, rhizomes and roots of the the Angelica archangelica L. and the Angelica sylvestris L., the content of 6 components of tannins (4 simple catechins – gallocatechin, epigallocatechin, catechin, epicatechin; 2 complex catechins – epicatechin gallate and catechin gallate) and free gallate were identified and determined. The highest content of components of tannins was observed in angelica leaves. No catechin gallate was detected in the leaves of the angelica, and free ellagic acid was found in the leaves of the Angelica sylvestris L. The rhizomes and roots of the Angelica archangelica L. and the Angelica sylvestris L. had the same composition of tannin components. They differed slightly only in quantitative content. Catechin and catechin gallate were not detected in both studied objects.

Conclusions. The component composition of the tannins of the Angelica archangelica L. and the Angelica sylvestris L. leaves, rhizomes and roots was investigated using the HPLC method. It was established that the raw materials of the studied species contain free gallic and ellagic acids, gallocatechin, epigallocatechin, catechin, epicatechin, epicatechin gallate, catechin gallate. The highest content of tannins was found in Angelica archangelica L. leaves. Such pharmacologically important substances as gallocatechin and epigallocatechin were found in significant quantities in Angelica archangelica L. leaves. The Angelica sylvestris L. leaves and underground organs of the studied species of the Angelica genus contained a much lower content of tannin components. The underground organs of the Angelica archangelica L. and the Angelica sylvestris L. contained the same composition of components of tannins, which differed in their quantitative content.