STUDY OF ANTHOCYANINS IN FRUITS OF LONICERA COERULEA
DOI:
https://doi.org/10.11603/mcch.2410-681X.2021.i1.12111Keywords:
Lonicera coerulea L, anthocyanins, identification, qualitative composition, quantitative contentAbstract
Introduction. World science has proven that fruits and berries are one of the most important components of the structure of a healthy diet, as they accumulate a large number of biologically active substances (BAS), without which human life is impossible. The ability of fruit, berry and non-traditional crops to provide the population with high quality products is indispensable for the prevention and treatment of many diseases. A special place among such non-traditional rare crops is occupied by species of the genus Lonicera Lindl. One of them is blue honeysuckle – a fruit and berry plant that is widely cultivated in Ukraine. No species of honeysuckle is yet included in the official register of medicinal plants, in the SPU, although their useful properties were known to our ancestors. Therefore, the possibility of their application in medical practice requires in-depth study of the qualitative composition and determination of the quantitative content of BAS groups.
The aim of the study – to identify and quantify the content of anthocyanins in fruit of Lonicera coerulea.
Research Methods. Identification of anthocyanins was performed by conventional reactions and thin layer chromatography (TLC), the content of anthocyanins in Lonicera coerulea fresh and dried fruits was determined by absorption spectrophotometry.
Results and Discussion. Analytical signals of the identification reactions indicated the presence of anthocyanins in fresh and dried fruits of Lonicera coerulea. The presence of cyanidin-3,5-diglucoside and cyanidin-3-O-glucoside was detected by TLC. The content of anthocyanins in fresh fruit of Lonicera coerulea was (0.42±0.03) %, in dried – (0.31±0.02) % in terms of cyanidin-3-O-glucoside. In order to standardize the raw material, it is possible to offer a standardized indicator – the content of anthocyanins in fruits of Lonicera coerulea is not less than 0.35 % in terms of cyanidin-3-O-glucoside and dry raw materials.
Conclusion. The presence of anthocyanins in honeysuckle blue fruits has been proved by conventional reactions and TLC method. The content of anthocyanins in honeysuckle fruits of fresh and dried fruits was determined by the method of absorption spectrophotometry. The research results will be used in the development of raw material standardization parameters. Honeysuckle blue fruit can be used for the development and production of medicinal substances, functional foods with complex vitamin and mineral action.
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