THE SIGNIFICANCE OF FREE RADICAL OXIDATION AND ANTIOXIDANT PROTECTION IN GUINEA PIG BRONCHI IN THE PATHOGENESIS OF EXPERIMENTAL BRONCHIAL ASTHMA AND EXPERIMENTAL STOMACH ULCER AND THEIR PHARMACOLOGICAL CORRECTION
DOI:
https://doi.org/10.11603/mcch.2410-681X.2021.i1.12108Keywords:
bronchial asthma, stomach ulcer, free-radical oxidation, antioxidant defence, thiotriazolineAbstract
Introduction. General practitioners often observe different combinations of diseases, including cases of asthma and stomach ulcer disease, which undoubtedly complicate the course of these diseases and treatment.
The aim of the study – to investigate the state of the prooxidant and antioxidant system (AOS) in the bronchi of guinea pigs in the development of experimental bronchial asthma (EBA) and experimental stomach ulcer (ESU) before treatment and after the use of the antioxidant thiotriazoline.
Research Methods. Experimental studies were performed on 64 guinea pigs (males) weighing 180–220 g, divided into 6 groups of 9 animals each, except the first (10 animals). Group I (control) included intact guinea pigs, group II – animals with experimental asthma and ESU (5th day), group III – guinea pigs on the 19th day of the combined model process, group IV – animals with EBA and ESU (26th day), group V – guinea-pigs on the 33rd day of EBA and ESU, group VI – included animals with EBA and ESU that were treated by thiotriazoline in the rate of 100 mg/kg intramuscularly since 23rd day of the experiment for 10 days.
Results and Discussion. The results of biochemical studies indicate that in the development of EBA and ESU there are characteristic signs of oxidative stress, which is manifested by excessive formation of lipoperoxidation products on the background of depression of the enzymatic link of the antioxidant system. The introduction of thiotriazoline led to a significant reduction in the content of diene conjugates and malonic dialdehyde in the bronchi, respectively, by 44.3 % (p1≤0.05) and 38.5 % (p1≤0.05) and elevation of level superoxide dismutase, catalase and glutathione peroxidase in the bronchi, respectively, on 32.1 % (р1≤0.05), 38.3 % (р1≤0.05) and 35.1 % (р1≤0.05) compared with the group of animals that were not exposed to this drug.
Conclusion. The results of treatment with thiotriazoline for 10 days showed a significant inhibition of excessive lipoperoxidation activity with a simultaneous increase in AOS activity in experimental asthma and ESU.
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