RESVERATROL AS A CYTOPROTECTIVE ACTION FOR INDUCED CARCINOGENESIS IN RATS

Authors

  • О. B. Rytsyk I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • L. S. Fira I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • P. H. Lykhatskyi I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY

DOI:

https://doi.org/10.11603/mcch.2410-681X.2021.i1.12103

Keywords:

cytolytic processes, endogenous intoxication, permeability of cell membranes, resveratrol

Abstract

Introduction. An important, fundamental task of modern experimental and clinical research in oncology is the study of existing and identification of new ones, including metabolic factors that contribute to the emergence and progression of the tumor process. In vitro and in vivo studies have shown that phytochemicals have potential antioxidant, anti-inflammatory and anti-carcinogenic properties. To date, the mechanisms of the anticancer activity of resveratrol, a natural polyphenol that has the ability to inhibit the proliferation of most human cancer cell lines, are being actively studied.

The aim of the study – to investigate the cytoprotective properties of resveratrol under conditions of dimethylhydrazine-induced oncological process.

Research Methods. The experiments were performed on male white rats, which were simulated for colon cancer by weekly subcutaneous administration of 1,2-dimethylhydrazine at a dose of 7.2 mg/kg of body weight for 30 weeks. The antioxidant resveratrol was administered intragastrically at a dose of 20 mg/kg daily for 7 months. The state of cytoplasmic cell membranes was studied by the activity of gamma-glutamyltranspeptidase and alkaline phosphatase. The degree of endotoxemia was assessed by determining the erythrocyte index of intoxication and the content of average weight molecules.

Results and Discussion. After administration of dimethylhydrazine to animals, an increase in the activity of gamma-glutamyltranspeptidase and alkaline phosphatase in the blood plasma was observed in all periods of the study, as well as their decrease in the liver of animals with carcinogenesis. Against the background of daily use of resveratrol, the activity of gamma-glutamyltranspeptidase decreased by 2 times, alkaline phosphatase by 2.3 times in relation to animals that were injected with dimethylhydrazine. In animals that received the antioxidant resveratrol, the permeability of the erythrocyte membrane decreased by 50.7 % compared with animals that did not receive it. The use of resveratrol led to a decrease in the content of molecules of the average mass of all fractions in the blood serum, which indicates a decrease in the manifestations of endogenous intoxication in the affected organism.

Conclusions. The results obtained confirm the cytoprotective properties of resveratrol, which are indicated by the restoration of the activity of membrane-dependent enzymes in rats with experimental carcinogenesis. It has been shown that under these conditions, the prophylactic use of resveratrol reduces the level of endogenous intoxication and helps to restore the permeability of erythrocyte membranes, which confirms its membrane-protective properties.

References

Mykhaylovych, Yu.Y., & Zhurbenko, A.V., & Sumkina, O.V. (2013). Praktychni aspekty vprovadzhennia skryninhu kolorektalnoho raku v Ukraini. Sotsialno-eko­nomichne obhruntuvannia [Practical aspects of intro­duction of colorectal cancer screening in Ukraine. Socio-economic substantiation]. Klynicheskaya onkologiya – Clinical Oncology, 3, 6-10 [in Ukrainian].

Kuznyetsova, H.M. & Lynchak, O.V., Danylov, M.O., Kotlyar, I.P., & Rybalchenko, V.K. (2013). Vplyv po­khidnykh dyhidropirolu ta maleimidu na stan pechinky i tovstoi kyshky shchuriv u normi ta v umovakh indukovanoho dymetylhidrazynom kolorektalnoho raku [The effect of dihydropyrrole and maleimide derivatives on the condition of the liver and colon of rats in normal and in conditions of dimethylhydrazine-induced colorectal cancer]. Ukr. biokhim. zhurn. – Ukr. Biochemistry Journal, 85(3), 74-84 [in Ukrainian].

Perse, M. & Cerar, М. (2005). The dimethylhydrazine induced colorectal tumours in rat-experimental colorectal carcinogenesis. Oncol., 39, 1, 61-70.

Filinska, O.M. & Yablonska, S.V., Lynchak, O.V., Burlaka, A.P., Ostrovska, H.V., Rybalchenko, T.V. (2010). Vplyv pokhidnoho maleimidu na rozvytok okysnoho stresu v pechintsi pry indukovanomu 1,2-dymetylhidrazynom kantserohenezi tovstoho kyshechnyka shchuriv [Influence of maleimide derivative on the development of oxidative stress in the liver in 1,2-dimethylhydrazine-induced carcinogenesis of the large intestine of rats]. Dopovidi Natsionalnoi akademii nauk Ukrainy – Reports of the National Academy of Sciences of Ukraine, 8, 185-190 [in Ukrainian].

Beijnen, J.H. & Schellens, J.H (2004). Drug interactions in oncology. Lancet Oncol., 5 (8), 489-496.

Baur, J.A. & Sinclair, D.A. (2006). Therapeutic potential of resveratrol: the in vivo evidence. Nat. Rev. Drug Discov., 5, 493-506.

Aziz, M.H. & Kumar, R, Ahmad, N. (2003). Cancer chemoprevention by resveratrol: in vitro and in vivo studies and the underlying mechanisms (review). Int. J. Oncol., 23,17-28.

Soroka, Yu.V. (2013). Sorbtsiina korektsiia zmin imunolohichnoi reaktyvnosti shchuriv za umov ekspery­mentalnoho kantserohenezu ta zastosuvannia khimio­terapevtychnykh chynnykiv [Sorption correction of chan­ges in immunological reactivity of rats under conditions of experimental carcinogenesis and application of chemotherapeutic factors]. Svit biolohii ta medytsyny – World of Biology and Medicine, 4, 82-86 [in Ukrainian].

Rybolovlev, Yu.R., & Rybolovlev, R.S. (1979). Dozirovaniye veshchestv dlya mlekopitayushchikh po konstantam biologicheskoy aktivnosti [Dosing of sub­stances for mammals according to the constants of biological activity]. Doklady AN SSSR – Reports of the USSR Academy of Sciences, 247, 6, 1513-1516 [in Russian].

Whitfield, J.B. (2001). Gamma glutamyl trans­ferase Crit. Rev. Clin. Lab. Sci., 38(4), 263-355. Review. PubMed PMID: 11563810.

Kamyshnikov, V.S. (2000). Spravochnik po kli­niko-biokhimicheskoy laboratornoy diagnostike: v 2-kh t. [Handbook of clinical and biochemical laboratory diagnostics: in 2 volumes]. Minsk: Belarus – Minsk: Belarus, 1, 495-2, 463 [in Russian].

Togaybayev, A.A., Kurguzkin, A.V., & Rikun, I.V. (1988). Metod opredeleniya endogennoy intoksikatsii [Method for determining endogenous intoxication]. Lab. Delo – Lab. Business, 9, 22-24 [in Russian].

Nikolskaya, V.A., Danilchenko, Yu.D., & Meme­tova, Z.N. (2013). Biokhimicheskiy aspekt rassmotreniya roli molekul sredney massy v organizme [Biochemical aspect of consideration of the role of average mass mole­cules in the body in the body]. Uchenyye zapiski Tav­richeskogo natsionalnogo universiteta im. V.I. Vernadskogo Ser.: Biologiya, khimiya – Scientific Notes of Taurida National University. VI Vernadsky Ser.: Biology, Che­mistry, 1 (65), 139-145 [in Ukrainian].

Gross, D. & Tolba, R. (2015). Ethics in animal-based research. Eur. Surg. Res., 55(1-2), 43-57.

Okeh, U. (2009). Statistical problems in medical research. East. Afr. J. Public. Health, 6 (1), 1-7.

Badzhinyan, S.A. (2016) Vliyaniye oksidativnogo stressa na organizm cheloveka [Influence of oxidative stress on the human body]. Meditsinskaya nauka Armenii NAN RA – Medical Science of Armenia NAS RA, LVI(2), 12-20 [in Russian].

Grivennikova, V.G. & Vinogradov, A.D. (2013). Generatsiya aktivnykh form kisloroda mitokhondriyami [Generation of reactive oxygen species by mitochondria]. Usp. biol. khim – Suc. Biological Chem., 53, 245-296 [in Russian].

Moroz, L.V., & Paliy, I.H., Tkachenko, T.V. (2008). Zastosuvannia preparatu Enteros hel u kompleksnii terapii patsiientiv iz hostrymy virusnymy hepatytamy iz suputnim dysbakteriozom kyshechnyka [Application of Enterosgel drug in complex therapy of patients with acute viral hepatitis with concomitant intestinal dysbacteriosis]. Vnutrennyaya medytsyna – Internal Medicine, 1 (7), 11-18 [in Russian].

Kulinich, O.S., & Domshyna, O.O., & Shtemen­ko, N.I. (2013). Moduliatsiia hepatotoksychnosti tsysplatynu klasternymy spolukamy reniiu (III) u modeli kan­tserohenezu [Modulation of cisplatin hepatotoxicity by rhenium (III) cluster compounds in a carcinogenesis model]. Medychna khimiia – Medical Chemistry, 3, 21-26 [in Ukrainian].

Vitkina, T.I. (2014). Sredniye molekuly v otsenke urovnya endogennoy intoksikatsii pri khronicheskom neobstruktivnom bronkhite [Medium molecules in assessing the level of endogenous intoxication in chronic non-obstructive bronchitis]. Zdorovye. Meditsinskaya ekologiya. Nauka – Health. Medical Ecology. The Science, 56 (2), 70-72 [in Russian].

Karyakina, Ye.V. & Belova, S.V. (2004). Molekuly sredney massy kak integralnyy pokazatel metabolicheskikh narusheniy (obzor literatury) [Average mass molecules as an integral indicator of metabolic disorders (literature review)]. Klin. lab. diagnostika – Сlin. Lab. Diagnostics, 3, 3-8 [in Ukrainian].

Matveyev, S.B., Fedorova, N.V., & Godkov, M.A. (2009). Otsenka endogennoy intoksikatsii po pokazatelyam srednemolekulyarnykh peptidov pri neotlozhnykh sostoyaniyakh [Evaluation of endogenous intoxication by indicators of medium molecular peptides in emergency conditions]. Klin. lab. diagnostika – Clin. Lab. Diagnostics, 5, 16-18 [in Ukrainian].

Published

2021-05-22

How to Cite

Rytsyk О. B., Fira, L. S., & Lykhatskyi, P. H. (2021). RESVERATROL AS A CYTOPROTECTIVE ACTION FOR INDUCED CARCINOGENESIS IN RATS. Medical and Clinical Chemistry, (1), 13–20. https://doi.org/10.11603/mcch.2410-681X.2021.i1.12103

Issue

Section

ORIGINAL INVESTIGATIONS