NEURONAL CELL ADHESION MOLECULES AND INDICATORS OF OXIDATIVE STRESS IN THE HEART OF RATS WITH HYPERGLYCEMIA AND MELATONIN IMPACT

Authors

  • Y. P. Kovalchuk OLES HONCHAR DNIPRO NATIONAL UNIVERSITY
  • G. Yu. Pavlenko OLES HONCHAR DNIPRO NATIONAL UNIVERSITY
  • S. V. Kyrychenko OLES HONCHAR DNIPRO NATIONAL UNIVERSITY
  • V. I. Zhyliuk DNIPRO STATE MEDICAL UNIVERSITY
  • G. O. Ushakovа OLES HONCHAR DNIPRO NATIONAL UNIVERSITY

DOI:

https://doi.org/10.11603/mcch.2410-681X.2021.i1.12022

Keywords:

neural cell adhesion molecule, oxidative stress, heart, hyperglycemia, rats

Abstract

Introduction. Today, it is known that diabetes causes serious pathological changes in the microcirculatory tract in many organs and tissues and at the same time is associated with an increased risk of coronary and peripheral macrovascular pathologies. Oxidative stress, which occurs in body tissues under conditions of chronic hyperglycemia, and the weakening of antioxidant activity is the trigger for the development of diabetic complications. Physiological activity of the myocardium depends on the regulation of the CNS.

The aim of the study – to investigate the change in the content of the neuronal molecule of cell adhesion and oxidative stress in the heart of rats under conditions of hyperglycemia and exposure to melatonin.

Research Methods. The study was performed on adult male Wistar rats. Animals were divided into 3 groups (n=7): 1 – control; 2 – animals with induced type 2 diabetes mellitus; 3 – animals that received melatonin after induction of diabetes mellitus type 2. The level of total protein was determined due to Bradford. The content of malonic dialdehyde was determined photometrically by reaction with thiobarbituric acid. Superoxide dismutase activity was determined by the oxidation reaction of quercetin. The content of NCAM was determined according to enzyme-linked immunosorbent assay. Statistical evaluation of the data was performed by one-way analysis of variance ANOVA.

Results and Discussion. Changes in the content of the neuronal cell adhesion molecule (NCAM) and mar­kers of oxidative stress in the heart of rats under hyperglycemia were found. The content of the membrane form of the neuronal cell adhesion molecule in the heart of rats with induced type 2 diabetes had statistically supported tendency to increase. At the same time, there was a significant increase of the MDA content, as well as a decrease of the activity of superoxide dismutase and catalase in the heart of rats with type 2 diabetes, which is a characteristic feature of oxidative stress. The application of melatonin after induction of type 2 diabetes resulted in a decrease in the content of NCAM in the heart of experimental rats, compared with animals with induced type 2 diabetes.

Conclusions. Oxidative stress is the cause of metabolic disorders in myocardial tissues, which leads to the appearance and development of diabetic complications. NMCA can act as a compensatory cardioprotective factor that is activated under metabolic stress. A positive effect of melatonin in the protection of membrane adhesive protein metabolism in the heart of rats under conditions of hyperglycemia.

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Published

2021-05-22

How to Cite

Kovalchuk, Y. P., Pavlenko, G. Y., Kyrychenko, S. V., Zhyliuk, V. I., & Ushakovа G. O. (2021). NEURONAL CELL ADHESION MOLECULES AND INDICATORS OF OXIDATIVE STRESS IN THE HEART OF RATS WITH HYPERGLYCEMIA AND MELATONIN IMPACT. Medical and Clinical Chemistry, (1), 54–62. https://doi.org/10.11603/mcch.2410-681X.2021.i1.12022

Issue

No. 1 (2021)

Section

ORIGINAL INVESTIGATIONS

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