STUDY OF AMINO ACIDS CENTAUREA CYANUS L.
Keywords:blue cornflower, amino acids, ion exchange liquid column chromatography
Introduction. Therapeutic use of amino acids is a promising area of modern pharmacy and medicine. Amino acids play an important role in the complex pharmacological action of herbal medicines, as well as improve the absorption of other biologically active substances and potentiate their pharmacological activity.
The aim of the study – to learn the qualitative composition of amino acids and determine their content in the herb and flowers of blue cornflower.
Research Methods. The amino acid composition of raw blue cornflower was studied by ion-exchange liquid column chromatography on an automatic amino acid analyzer AAA T-339M.
Results and Discussion. As a result of the study, 18 amino acids with a total content of 4.77 mg/100 mg and 5.41 mg/100 mg, respectively, were identified in herb and flowers of blue cornflower. Blue cornflower flowers contained 31.34 %, herb – 25.70 % of essential amino acids from the sum of amino acids. Among the essential amino acids in the herb, proline (0.94 mg/100 mg), glutamic (0.70 mg/100 mg) and aspartic (0.64 mg/100 mg) acids predominated, and leucine among the essential ones – 0.27 mg/100 mg) and lysine (0.24 mg/100 mg). In flowers, aspartic acid (0.82 mg/100 mg), glutamic acid (0.68 mg/100 mg) and proline (0.53 mg/100 mg) were the dominant substitutes for lysine (0.46 mg/100 mg), leucine (0.36 mg/100 mg) and threonine (0.28 mg/100 mg).
Conclusions. For the first time, the qualitative composition and quantitative content of amino acids in the herb and flowers of blue cornflower harvested in Kharkiv region were studied. The study was performed by ion exchange liquid chromatography. It was determined that a slight advantage of the total number of amino acids is characteristic of blue cornflower flowers. In both studied raw materials, blue cornflower was found to have the advantage of substituted amino acids (3.55–3.72 mg/100 mg), in particular glutamic, asparagine and proline. The obtained results can be used in the development of criteria for standardization of raw blue cornflower, as well as in the production of drugs of plant origin on its basis of multi-vector pharmacological activity.
Lockowandt, L., Pinela, J., Roriz Lobo, Cu., Pereira, C., Abreu, R.M.V., Calhelha, R.C., Alves, M.J., Barros, L., Bredol, M., & Ferreira, I.C.F.R. (2019). Chemical features and bioactivities of cornflower (Centaurea cyanus L.) capitula: The blue flowers and the unexplored non-edible part. Industrial Crops & Products, 128, 496-503.
Fernandes, L., Ramalhosa, E., Pereira, J.A., Saraiva, J.A., & Casal, S. (2020). Borage, camellia, centaurea and pansies: Nutritional, fatty acids, free sugars, vitamin E, carotenoids and organic acids characterization. Food Research International, 132, 109070. DOI:10.1016/j.foodres.2020.109070.
Odia, A., & Esezobor, O.Z. (2017). Therapeutic uses of amino acids. In book: Amino Acid – New Insights and Roles in Plant and Animal. Publisher: InTechEditors: Toshiki Asao and Md. Asaduzzaman.
Wu, G. (2009). Amino acids: metabolism, functions, and nutrition. Amino Acids, 37 (1), 1-17. DOI: 10.1007/s00726-009-0269-0.
Drozdova, I.L., & Lupilina, T.I. (2015). Aminokislotnyi sostav travy ikotnika serogo [Amino acid composition of gray hiccup herb.]. Vestnik VGU, Serija: Himija. Biologija. Farmacija, 1, 125–128 [in Russian].
Waheed, E.J., Obaid, S.M.H., & Al-Hamdani, A.A.S. (2019). Biological activities of amino acid derivatives and their complexes a review. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 10 (2), 1624-1641.
Kochikyan, A.T., Kochikyan, V.T., & Topchyan, A.V. (2011). Aminokislotnyy sostav nekotorykh pishchevykh i lekarstvennykh rasteniy flory Armenii [Amino acid composition of some food and medicinal plants of the flora of Armenia]. Meditsinskaya nauka Armenii NAN RA – Medical Education of Armenia of the NAS of RA, 3, 119-131 [in Russian].
Delian, Ye.P. (2016). Aminokyslotnyi sklad nadzemnykh orhaniv roslyn rodu Sonchus [Amino acid composition of overground organs of plant genus Sonchus]. Farmakolohiia ta likarska toksykolohiia – Pharmacology and Doctor’s Toxicology, 1 (47), 102-106 [in Ukrainian].
Shimorova, Y.Ye., Кyslychenko, V.S., & Horiacha, L.M. (2020). Izuchenie aminokislotnogo sostava pasternaka posevnogo (Pastinaca sativa L.) [Amino acids composition study of parsnip (Pastinaca sativa L.)]. Norwegian Journal of Development of the International Science, 38, 42-45 [in Russian].
Iosypenko, O.O., Кyslychenko, V.S., & Omelchenko, Z.І. (2020). Vyvchennia aminokyslotnoho skladu lystia kabachkiv [Study of the amino acid composition of zucchini leaves]. Medychna ta klinichna khimiia – Medical and Clinical Chemistry, 22 (2), 72-80 [in Ukrainian].