STUDY OF AMINO ACIDS CENTAUREA CYANUS L.
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.v.i3.11545Keywords:
blue cornflower, amino acids, ion exchange liquid column chromatographyAbstract
Introduction. Therapeutic use of amino acids is a promising area of modern pharmacy and medicine. Amino acids play an important role in the complex pharmacological action of herbal medicines, as well as improve the absorption of other biologically active substances and potentiate their pharmacological activity.
The aim of the study – to learn the qualitative composition of amino acids and determine their content in the herb and flowers of blue cornflower.
Research Methods. The amino acid composition of raw blue cornflower was studied by ion-exchange liquid column chromatography on an automatic amino acid analyzer AAA T-339M.
Results and Discussion. As a result of the study, 18 amino acids with a total content of 4.77 mg/100 mg and 5.41 mg/100 mg, respectively, were identified in herb and flowers of blue cornflower. Blue cornflower flowers contained 31.34 %, herb – 25.70 % of essential amino acids from the sum of amino acids. Among the essential amino acids in the herb, proline (0.94 mg/100 mg), glutamic (0.70 mg/100 mg) and aspartic (0.64 mg/100 mg) acids predominated, and leucine among the essential ones – 0.27 mg/100 mg) and lysine (0.24 mg/100 mg). In flowers, aspartic acid (0.82 mg/100 mg), glutamic acid (0.68 mg/100 mg) and proline (0.53 mg/100 mg) were the dominant substitutes for lysine (0.46 mg/100 mg), leucine (0.36 mg/100 mg) and threonine (0.28 mg/100 mg).
Conclusions. For the first time, the qualitative composition and quantitative content of amino acids in the herb and flowers of blue cornflower harvested in Kharkiv region were studied. The study was performed by ion exchange liquid chromatography. It was determined that a slight advantage of the total number of amino acids is characteristic of blue cornflower flowers. In both studied raw materials, blue cornflower was found to have the advantage of substituted amino acids (3.55–3.72 mg/100 mg), in particular glutamic, asparagine and proline. The obtained results can be used in the development of criteria for standardization of raw blue cornflower, as well as in the production of drugs of plant origin on its basis of multi-vector pharmacological activity.
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