ENDOGENOUS INTOXICATION OF EXPERIMENTAL ANIMALS AT DAMAGE OF THE TESTES BY CADMIUM CHLORIDE
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.v.i2.11353Keywords:
testes, endogenous intoxication, cadmium chlorideAbstract
Introduction. It is known that structural changes and endogenous intoxication at damage of the testes by harmful factors of exogenous origin have been insufficiently studied.
The aim of the study – to investigate the features of changes of endogenous intoxication at damage of the testes by cadmium chloride.
Research Methods. Biochemical and morphological methods were used to study the testes of 30 white rats, which were divided into 2 groups. Group 1 consisted of 15 intact animals, group 2 – 15 rats, which were injected subcutaneously with cadmium chloride at a dose of 6 mg/kg for 4 weeks. One month after the start of the experiment, euthanasia of experimental animals was performed by bloodletting under conditions of thiopental anesthesia. The content of medium weight molecules, the concentration of TBA-active products and oxidative modification of proteins were determined in the serum of rats. Histological micronutrients were made from the testes and studied morphometrically. Correlation analysis was performed between biochemical and morphometric parameters.Quantitative indicators were processed statistically.
Results and Discussion. Prolonged exposure to cadmium chloride to the body led to a significant increase in serum molecules of medium weight and an increase in TBA-active products by 48.3 % (p<0.001), oxidative modification of proteins in the serum of rats, that was recorded at a wavelength of 370 nm – 7.4 times (p<0.001), and when determining their wavelength 430 nm – 7.68 times (p<0.001). The diameter of the seminal tubules decreased by 32.6 % (p<0.001), the number of epithelial-spermatogenic layer cells – by 31.3 % (p<0.001), Sertoli cells – by 11.3 % (p<0.001), tubulo-interstitial index – by 26.8 % (p<0.001), the index of spermatogenesis intensity – by 38.8 % (p<0.001), and the wall thickness of the seminal tubules increased by 23.6 % (p<0.001), the Leydig index – by 56.1 % (p<0.001), the relative volume of damaged spermatogenic epitheliocytes – 19.6 times (p<0.001). Strong, significant positive and negative correlations were found between the indicators of endogenous intoxication and morphometric parameters of testicular structures, which confirmed the dependence of the degree of endogenous intoxication on the severity and prevalence of morphological changes.
Conclusion. The introduction of cadmium chloride into the body of experimental animals leads to severe damage of the testes and a significant increase in endogenous intoxication. Strong, significant positive and negative correlations have been established between the degree of endogenous intoxication disorders and the studied morphometric parameters of testicular structures, which confirms the dependence of the severity of endogenous intoxication on the prevalence of morphological changes.
References
Dubinina, A.A. (2016). Toksychni rechovyny і metody yikh vyznachennia [Toxic substances and methods for their determination]. Kharkiv: KHDUKHT [in Ukrainian].
Bazalytska, S.V. (2016). Cholovicha neplidnist v Ukraini: osoblyvosti pato- i morphohenezu [Male infertility in Ukraine: features of pathogenesis and morphogenesis]. Kyiv: TOV “Chetverta khvylia” [in Ukrainian].
Hnatyuk, M.S., Konovalenko, S.O., & Tatarchuk, L.V. (2019). Osoblyvosti remodeliuvannia sudyn hemomikrotsyrkuliatornoho rusla yaiechok pry postrezektsiinii portalnii hipertenzii [Peculiarities of vascular remodeling of hemo-microcirculatory bed of testes at postresection portal hypertension]. Shpytalna khirurhiia – Hospital Surgery, 4, 37-42 [in Ukranian].
Dolynko, N.P. (2015). Vliyanie ginko biloba na morfometricheskie i funktsionalnye pokazateli spermatozoidov v usloviyakh khronicheskoy etanolovoy intoksikatsii [The influence of ginkgo biloba on the morphometric and functional indicator of spermatozoa in conditions of chronic ethanol intoxication]. Vestnik Mogelevskogo derzhavnogo universytetu im. A.A.Kulyashova. Seriya Pryrodnychi nauky – Bulletin of Mogilev State University by A.A. Kuleshov. Natural Science Series, 2 (46), 99-102 [in Russian].
Konovalenko, S.O., Lototskyi, V.V., & Tatarchuk, L.V. (2016) Strukturni zminy v yaiechku pry dii na orhanism khlorydu kadmiiu [Structural changes in the testis when exposed to cadmium chloride]. Problemy viiskovoi okhorony zdorovia – Problems of Military Health Care, 45, 199-206 [in Ukrainian].
Koryakina, Ye.V., & Belov, S.V. (2004). Molekuly sredney massy kak integralnyy pokazatel metabolicheskikh narusheniy [Molecules of the average mass as an integral index of metabolic disturbances]. Klin. i lab. diagnostika – Clinical and Laboratory Diagnostics, 3, 3-8 [in Russian].
Stalnaya, I.D., & Garishvily, T.G. (1977). Metod opredeleniya malonovogo aldegida s pomoshchyu tiobarbiturovoy kisloty [The method of determination of malonic aldehyde with tiobarbituric acid]. Sovremennyye metody v biokhimii – Modern Methods in Biochemistry. Moscow: Meditsina [in Russian].
Meshchyken, I.F. (1988). Metod vyznachennia okysliuvalnoi modyfikatsii bilkiv plazmy (syrovatky) krovi [The method of determination of oxidative modification of plasma proteins (serum)]. Bukov. Med. Visnyk – Bukovinian Medical Journal, 1,156-157 [in Ukrainian].
Goralsky, L.P., Khomich, V.T., & Kononsky, O.I. (2011). Osnovy histolohichnoi tekhniky i morfofunktsionalni metody doslidzhen u normi i pry patolohii [Fundamentals of histological technique and morphofunctional methods of research in norm and pathology]. Zhytomyr: Polissia [in Ukrainian].
Avtandylov, G.G. (2002). Osnovy kolychestvennoy patologicheskoy anatomii [Basis of quantative pathological anatomy]. Moscow: Meditsyna [in Russian].
Reznykov, O.H. (2003). Zahalni etychni pryntsypy eksperymentiv na tvarynakh [General ethical principles experiments on animals]. Endokrynolohiia – Endocrinology,8, 1, 142-145 [in Ukrainian].
Lapach, S.N., Gubenko, A.V., & Babych, P.N. (2001). Statisticheskye metody v miediko-biolohicheskykh issledovanyiakh Excell [Statistical methods in biomedical research Excell]. Kyiv: Morion [in Ukrainian].
