THE STATE OF THE PROOXIDANT-ANTIOXIDANT SYSTEM IN THE LIVER OF ALLOXAN DIABETIC RATS IN THE CONDITIONS OF MELATONIN INTRODUCTION
DOI:
https://doi.org/10.11603/mcch.2410-681X.2019.v.i2.10304Keywords:
alloxan diabetes mellitus, melatonin, liver, prooxidant-antioxidant systemAbstract
Introduction. An experimental model of alloxan diabetes mellitus is often used to study the pathogenesis of diabetes. In diabetes, oxidative stress is increased, which leads to depletion of the antioxidant system. Melatonin is an antioxidant and activates the body's defenses, protecting it from diseases associated with the activation of free radical processes.
The aim of the study – to determine the effect of melatonin on the prooxidant and antioxidant status in the liver of alloxan diabetic rats.
Research Methods. Experiments were conducted on white outbred sexually mature male rats. The content of oxidatively modified proteins, TBA-active products, the activity of catalase and superoxide dismutase (SOD) was determined in the liver.
Results and Discussion. The results of our research showed that under the conditions of alloxan diabetes mellitus is activation of free radical oxidation of biomolecules, as evidenced increase the content of TBA-products and oxidatively modified proteins on the 7th and 14th days of the experiment in the liver of alloxane diabetic rats. At the same time, decrease of activity of catalase and superoxide dismutase is observed in the liver of rats in diabetic group. The positive effect of melatonin is shown in the decrease the content of TBA-active products and oxidatively modified proteins in the liver compared to group of alloxan diabetic animals. In addition, the use of melatonin contributed to the normalization the activity of antioxidant enzymes in rat liver with alloxan diabetes: increase the activity of catalase and superoxide dismutase compared to diabetic group.
Conclusion. In the conditions of exogenous melatonin introduction to rats with alloxan diabetes in a dose of 10 mg/kg daily for 7 and 14 days cause a pronounced antioxidant effect, reducing free radical oxidation and normalizing the activity of enzymes of antioxidant defense in the liver of alloxan diabetic rats.
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