CHANGES OF THE PROOXIDANT-ANTIOXIDANT SYSTEM ACTIVITY IN THE EARLY PERIOD OF POLYTRAUMA DEVELOPMENT AND PENTOXIFYLLINE CORRECTION

  • O. O. Kulianda I. HORBACHEVSKY TERNOPIL STATE MEDICAL UNIVERSITY
Keywords: polytrauma, oxidative stress, pentoxifylline

Abstract

Introduction. Trauma is one of the causes of oxidative stress. Membrane stabilizing, tread, anti-calcium, antianginal and anti-aggregant properties of xanthines, in particular pentoxifylline, along with other inherent pharmacodynamic effects, can prevent the formation of irreversible pathological changes in various organs and tissues.

The aim of the study – to determine the peculiarities of the changes of the prooxidant-antioxidant system in the early period of polytrauma development and the effectiveness of using pentoxifylline.

Research Methods. Experiments were conducted on white, non-breeding, sexually mature male rats. The content of malondialdehyde and lipid hydroperoxides was determined in the liver tissue and blood plasma. The level of catalase and ceruloplasmin activity in the liver and blood plasma were determined. For the purpose of correction, pentoxifylline was used. The drug was injected intraperitoneally once a day in the form of 2 % aqueous solution at a dose of 25 mg/kg for 7 days.

Results and Discussion. The use of pentoxifylline in experimental animals with a model of polytrauma led to a decrease in the concentration of lipid hydroperoxides and malonic dialdehyde in the blood plasma already from the 1st day of the study. The introduction of pentoxifylline caused a significant decrease in the content of lipid hydroperoxides and malondialdehyde in the liver of animals during the entire observation period. A statistically significant decrease in plasma catalase activity was noted in the first and third days of the experiment. Its increase in liver tissue was observed from the 3rd day of the study. No statistically significant effect on the level of ceruloplasmin in the liver and plasma after administration of pentoxifylline was detected.

Conclusion. Under the conditions of experimental polytrauma and introduction of pentoxifylline in the form of 2 % aqueous solution at a dose of 25 mg/kg within 7 days, the decrease in the activity of the prooxidant system and a significant increase in the activity of catalase in liver parenchyma were observed.

References

(2015). Influence of antioxidant therapy on the clinical status of multiple trauma patients. A retrospective single center study. Rom. J. Anaesth. Intensive Care, 22 (2), 89-96.

Bedreag, O.H., Rogobete, A.F., Sarandan, M., Cradigati, A.C., Papurica, M., Dumbuleu, M.C., … & Sandesc, D. (2015). Oxidative stress in severe pulmonary trauma in critical ill patients. Antioxidant therapy in patients with multiple trauma-A review. Anaesthesiol. Intensive Ther., 47 (4), 351-359. Retrieved from: https://doi.org/10.5603/AIT.a2015.0030

Sies, H. (2015). Redox biology oxidative stress: a concept in redox biology and medicine. Redox Biol., 4, 180-183.

Espinosa-Diez, C., Miguel, V., Mennerich, D., Kietzmann, T., SánchezPérez, P., Cadenas, S., & La­mas, S. (2015). Antioxidant responses and cellular adjust­ments to oxidative stress. Redox Biol., 6, 183-197. Re­trie­ved from: https://doi.org/10.1016/j.redox.2015.07.008.

Bedreag, O.H., Rogobete, A.F., Sarandan, M., Cradigati, A.C., Papurica, M., & Dumbuleu, M.C. (2015). Oxidative stress in severe pulmonary trauma in critical ill patients. Antioxidant therapy in patients with multiple trauma-A review. Anaesthesiol. Intensive Ther., 47 (4), 351-359. Retrieved from: https://doi.org/10.5603/AIT.a2015.0030.

Kozak, D.V. (2013). Lipid peroxidation, antioxidant defence, endogenous intoxication and cytolysis variations in early and late manifestations of traumatic disease. J. Health Sci., 3, 4, 108-117.

Neves, K.R., Nobre, H.V.Jr., Leal, L.K., de Andra­de, G.M., Brito, G.A., & Viana, G.S. (2015). Pentoxifylline neuroprotective effects are possibly related to its anti-inflammatory and TNF-alpha inhibitory properties, in the 6-OHDA model of Parkinson’s disease. Parkinsons Dis., 108179.

Mozaffari, S., & Abdollahi, M. (2013). A review on the role of oxidative stress and inflammation in necrotizing enterocolitis and benefits of the phosphodiesterase inhibitor pentoxifylline. International Journal of Pharma­cology, 9 (4), 245-250.

Babaei, S., Bayat, M., Nouruzian, M., & Bayat, M. (2013). Pentoxifylline improves cutaneous wound healing in streptozotocin-induced diabetic rats. Eur. J. Pharmacol., 700, 165-172.

Halis, H., Liman, N., Baştuğ, O., & Güneş, T. (2017). The role of pentoxifylline on neuroprotection in neonatal rat model of hypoxic ischemic brain injury. Curr. Pediatr. Res., 21 (1), 77-85.

Kulianda, O.O. (2013). Dynamika profiliu tsyto­kiniv pry zastosuvanni pentoksyfilinu v umovakh poli­travmy [Dynamics of the profile of cytokines in the use of pentoxifylline in the conditions of polytrauma]. Klinich. khirurhiia – Clinical Surgery, 12, 73-75 [in Ukrainian].

Pat. 63997 Ukraina, MPK G 09 V 23/28. Sposib modeliuvannia politravmy. Kozak D.V. ; zaiavnyk i paten­tovlasnyk Ternopil. derzh. med. un-t imeni I. Ya. Hor­bachevskoho. – № u 201104110 ; zaiavl. 05.04.11 ; opubl. 25.10.11, Biul. № 20. 14. [Patent 63997 Ukraine, IPC G 09 at 23/28. Method of modeling polytrauma. Kozak D.V. ; Applicant and patent owner of I. Horbachevsky Ternopil State Medical University. No. u 201104110; stated. 05/04/11; published October 25, 10, Bull. No. 20]. [in Ukrainian].

Pat. 86467 Ukraina, MPK (2013.01) A61 B 17/00. Sposib korektsii balansu tsytokiniv pry politravmi v eksperymenti / Korda M. M., Kulyanda O. O., Kulyanda I. S., Koptyukh V. V. ; zaiavnyk i patentovlasnyk Ternopil. derzh. med. un-t imeni I. Ya. Horbachevskoho. – № u 2013 09854 ; zaiavl. 08.08.13 ; opubl. 25.12.13, Byul. № 24 [Patent 86467 Ukraine, IPC (2013.01) А61 B 17/00. Method of correction of balance of cytokines with polytrauma in experiment / Korda, M.M., Kulianda O.O., Kulianda, I.S., Koptiuk, V.V.; Applicant and patent owner of I. Horbachevsky Ternopil State Medical Uni­versity. No. u 2013 09854; stated. 08.08.13; published 25.12.13, Bul. No. 24 [in Ukrainian].

Andreyeva, L.I., Kozhemyakin, L.A., & Kish­kun, A.A. (1988). Modifikatsiya metoda opredeleniya perekisey lipidov v teste s tiobarbiturovoy kislotoy [Modi­fication of the method for determining lipid peroxides in a test with thiobarbituric acid]. Lab. delo – Laboratory Work, 11, 41-43 [in Russian].

Gavrilov, V.B., & Mishkorudnaya, M.I. (1983). Spektrofotometricheskoye opredeleniye gidroperekisey lipidov v plazme krovi [Spectrophotometric determination of lipid hydroperoxides in blood plasma]. Lab. delo. – Laboratory Work, 3, 33-35 [in Russian].

Korolyuk, M.A., Ivanova, L.I., Mayorova, N.G., & Tokarev, V.Ye. (1988). Metod opredeleniya aktivnosti katalazy [Method for determining catalase activity]. Lab. delo – Laboratory Work, 1, 16-19 [in Russian].

Published
2019-07-11
How to Cite
Kulianda, O. O. (2019). CHANGES OF THE PROOXIDANT-ANTIOXIDANT SYSTEM ACTIVITY IN THE EARLY PERIOD OF POLYTRAUMA DEVELOPMENT AND PENTOXIFYLLINE CORRECTION. Medical and Clinical Chemistry, (2), 91-96. https://doi.org/10.11603/mcch.2410-681X.2019.v.i2.10300
Section
ORIGINAL INVESTIGATIONS