INVESTIGATION OF STEROID COMPOUNDS OF AJUGA REPTANS L. HERB
Introduction. Nowadays, the study of plants which are widespread not only in Ukraine but also throughout the globe is relevant. One of these plants is Ajuga reptans L. It is known that in folk medicine Ajuga reptans L. is used in treating of inflammatory diseases of the gastrointestinal tract. Steroids are a class of biologically active compounds that exhibit anti-inflammatory activity. Literature data do not consist any sources on the content of steroids in the Ajuga reptans L. herb. That is why the study of this group of compounds is promising.
The aim of the study – to conduct a study on the identification and determination of the quantitative and qualitative content of steroid compounds of Ajuga reptans L. herb. (Ajuga reptans L.) using gas chromatography/ mass spectrometry
Research Methods. The object of the study was the herb of Ajuga reptans L. harvested during the flowering period in the village Huta of Bohorodchany district in 2017–2018 years. The method of gas chromatography-mass spectrometry has been used to study the content of steroid compounds in the Ajuga reptans L.herb. The research was carried out on the Agilent Technologies 6890 chromatograph with a mass spectrometric detector 5973 and the capillary column HP-5 ms (0.25 mm in diameter, 30 m in length). The obtained data were recorded using figures and tables.
Results and Discussion. These studies indicate the presence of 54 substances, 9 of which have been identified: stigmasterol; pregna-5,17-dien-3-ol; ergosterol; 3β-stearyloxy-ers-12-ene; stigmasta-5,22-dien-3-ol, acetate; β-sitosterol; olean-12-ene; 3β-methoxy-5-cholestene; traces of cholesterol. It was established that stigmasterol has the highest concentration. Its content is 1546.20 mg/kg of raw materials or 44.28 % of the content of all the identified steroids. A significant content of pregna-5,17-dien-3-ol was found – 843.91 mg/kg or 24.17 % of all steroids of Ajuga reptans L. herb. The total content of steroids in the Ajuga reptans L. herb is 3491.96 mg/kg.
Conclusions. The method of gas chromatography-mass spectrometry was used to determine the qualitative composition and quantitative content of steroids of Ajuga reptans L. herb. The analysis of the obtained results shows that the steroid complex of Ajuga reptans L. herb is represented by 54 steroids, 9 of which were identified and quantified. The obtained data will be taken into account for further production of the herbal drugs based on Ajuga reptans L. herb as well as the standardization parameters of the raw material.
Sytoprostat. Kompaniia “Tabletky. Ua” [Sitoprostatus. The company "Tablets. ua.] Retrieved from: http://pharmplanet.com.ua/en/producty-sitoprostat/ [in Ukrainian].
Kholesteryn ta yoho efiry [Cholesterol and its esters]. Retrieved from: http://www.terra-aromatica.ru/lipidy-rogovogo-sloya-holesterin-ego-efiry-alternativnoe-ispolzovanie-fitosterolov-i-35.html [in Ukrainian].
Beta-sytosterin rol u zdorovi ludyny, food and health. [Beta-sitosterol role in human health, food and health]. Retrieved from: http://jak.bono.odessa.ua/articles/beta-sitosterin-rol-u-zdorov-i-ljudini-food-and.php [in Ukrainian].
Ericson-Neilsen, W. (2014). Steroids: pharmacology, complications, and practice delivery issues. Ochsner J., 14, 203-207.
Patel, D.K., Kumar, R., & Prasad, S. Hemalatha (2011). Pharmacologically screened aphrodisiac plant – A review of currentscientific literature. Asian Pacific Journal of Tropical Biomedicine, 1 131-138.
Hurieva, I.H. (2014). Vyvchennia steroidnykh spoluk ta koreniv tyfonu [Study of steroid compounds and roots of typhon]. Ternopil. Fitoterapiia [in Ukrainian].
Racette, S.B., Lin, X., Lefevre, M., Spearie, C.A., Most, M.M., Ma, L. et al. (2010). Dose effects of dietary phytosterols on cholesterol metabolism: a controlled feeding study. Am. J. Clin. Nutr., 1, 71-73.
Voloshina, A.A., Kislichenko, V.S., Zhuravel, I.A., & Wash, N.E. (2013). Steroidnyye soyedineniya syrya Verbascum thapsus [Steroid compounds of Verbascum thapsus L.]. Retsept – Recipe, 2 (88), 95-99 [in Russian].
Tymofieieva, S.V., Kyslychenko, A.A., & Zhuravel, I.O. (2017). Vyvchennia steroidnykh spoluk u syrovyni KANNY SADOVOI [Study of steroid compounds in raw CANNY GARDEN]. Scientific Journal “ScienceRise: Pharmaceutical Science”, 2 (6), 38-41 [in Ukrainian].
Paziuk, D.-M. V., Zhuravel, I.O., Kyslychenko, A.A., & Bourda, N.E. (2017). Vyvchennia steroidnykh spoluk u syrovyni morkvy posivnoi sortiv "Yaskrava" ta "Nantska Kharkivska [Study of steroid compounds in the raw carrots of the seedlings "Yaskrava" and "Nantska Kharkivska"]. Fitoterapiia. Chasopys – Phytotherapy, Journal, 1, 31-33 [in Ukrainian].
Burda, N.Ye. (2013). Vyvchennia steroidnykh spoluk hrybiv shyitake, reyshy ta korditsepsu [Study of the steroid compounds of mushroom shiites, Reichs and Cordyceps]. Fitoterapiia. Chasopys – Phytotherapy, Journal, 4, 67-69 [in Ukrainian].
Burda, N.Ye. (2014). Vyvchennia steroidnykh spoluk u syrovyni pivonii likarskoi sortiv “Alba plena” ta “Rosea plena” [Study of steroid compounds in raw pions of medicinal varieties "Alba plena" and "Rosea plena"]. Fitoterapiia. Chasopys – Phytotherapy, Journal, 1, 67-70 [in Ukrainian].
Kyslychenko, O.A., Dyakonova, Ya.V., & Khanin, V.A. (2008). Gas chromatography with mass-spectrometric detection of the essential oils from Achillea carpatica Blocki ex Dubovik and Echinacea pallida (nutt.) Nutt. Abstracts 6th International Symposium on Chromatography of Natural Products (ISCNP). Lublin.