DAILY URINE RENAL STATE UNDER ISCHEMIC-REPERFUSION SYNDROME OF LIMBS, ABDOMINAL INJURY WITH HYPOVOLEMIC SHOCK AND THEIR COMBINATION IN THE EARLY PERIOD OF TRAUMATIC DISEASE

Abstract

The aim of the work – to study the effect of ischemic-reperfusion syndrome on the functional state of the kidneys in the early post-traumatic period.

Material and Methods. In the experiment, there were 80 sexually mature white male Wistar rats, weighing 190–220 g on a standard vivarium diet.

All animals were divided into groups: control and 3 experimental (8 animals in each group). In animals of the first experimental group under the thiopental-sodium enzymes (40 mg·kg^-1) proximal to the left paws imposed a hemostatic tourniquet for 120 minutes. In the second experimental group, a combined injury to the abdominal cavity was simulated by applying two dose injections to the abdominal region; Hypovolemic shock was modeled by bloodletting from 20 to 22 % of the volume of circulating blood from the femoral vessels. In the third experimental group, a combined injury to the abdominal cavity and reperfusion of the extremities was simulated. Animals of the control group were administered only in anesthesia.

After 1, 3 and 7 days in experimental animals, the functional status of the kidneys was determined by the method of water loading. Diuresis and velocity of glomerular filtration were evaluated.

Animals of experimental groups were extracted from the experiment under conditions of thiopental sodium anesthesia by the method of total blood-flow from the heart.

Results and Discussion. In the group of animals, after the overhangs on the lower extremities, after the first day, the diuresis significantly decreases and has minimal values; furthermore, the index increased but did not reach the control group even after 7 days (33.6 % less, (p<0.05) in comparable to control). In the group of animals with trauma of the abdominal cavity and hypovolemic shock the index reached the minimum level in 3 days and actually remained at the same level by the seventh day. In the group of animals where the trauma of the abdominal cavity was combined, bleeding and overlapping of the harness on the lower extremities, there was a tendency towards constant decrease of the index throughout the observation time. Its minimum values ​were recorded after 7 days (less by 33.6 % (p<0.05) compared to control). The velocity of glomerular filtration decreased in all groups of experimental animals. The increase in the indicator during the observation time was determined in the group of animals with overlapping of harnesses, although even after 7 days it remained two times lower than control values. In the group of animals with trauma of the abdominal cavity and hypovolemic shock, the index reached the minimum values ​after 3 days and remained at this level until the end of the observation, which corresponds to the classic picture of pathophysiological changes in the model of combined trauma. The most pronounced changes were in animals with combined trauma and ischemic-reperfusion syndrome. Significant decrease of the index was observed during all control points of observation.