PRO- AND ANTIPEROXIDE PROCESSES IN RAT BLOOD SERUM DURING HEALING OF FULL-LAYER EXCISED LINEAR WOUNDS
DOI:
https://doi.org/10.11603/1811-2471.2026.v.i2.16206Keywords:
full-thickness cut linear wounds, melanin, superoxide dismutase, catalase, diene conjugates, TBA-active products, aldo derivatives, keto derivativesAbstract
Summary. The problem of wound healing is associated with a number of reasons: surgical incisions, trauma, wounds in wars, population aging, increasing levels of obesity and diabetes. Existing treatment methods are relatively effective, therefore there is a need for a more detailed study of the pathogenesis of the disease and the search for natural compounds to accelerate wound healing.
The aim – to determine the state of the pro- and antioxidant system of rats during the healing of full-layer excised linear wounds treated with a melanin preparation.
Material and Methods. The studies were conducted on white non-linear male rats (n=40). The animals were divided into 3 groups. Group 1 – intact control – a group of healthy rats without wound modeling; Group 2 – rats aged 4 months, full-thickness cut linear wounds were simulated, which were not treated with anything; Group 3 – rats aged 4 months, full-thickness cut linear wounds were simulated and they were treated daily with carbopol gel with melanin (0.5%). The state of the body’s antioxidant defense system was assessed by the activity of superoxide dismutase and catalase. The intensity of free radical processes was assessed by the accumulation of diene conjugates, TBA-active products and Schiff bases.
Results. It was established that in the group of animals with cut wounds, the levels of diene conjugates, TBA-active products and Schiff bases increased in the blood serum, and epithelialization of the wound surface occured after the 14th day. Treatment of the wound surface with the Melanin preparation accelerated epithelialization by 3 days and led to a decrease in the levels of diene conjugates, TBA-active products and Schiff bases to the normal range in intact animals. Superoxide dismutase activity in the blood serum of animals with cut wounds decreased, but when treating wounds with the Melanin preparation, it increased towards normal values. Catalase activity, on the contrary, increased, but when treating wounds with the Melanin preparation, it decreased towards control values. The content of products of oxidative modification of proteins – aldo- and keto- derivatives, both neutral and basic, in animals with cut wounds increased, but when treating wounds with the Melanin preparation, these indicators approached the normal range as in intact animals.
Conclusions. With cut wounds in rats, the content of lipid peroxidation products increased in the blood serum. Treatment of wound surfaces with a melanin preparation accelerated wound healing and led to almost complete normalization of lipid peroxidation indicators. In blood serum with cut wounds, the activity of superoxide dismutase decreased. Treatment of wounds with a melanin preparation led to acceleration of complete epithelialization of the wound surface and an increase in enzyme activity towards the control values of intact animals. In the blood serum of rats with cut linear wounds, the level of oxidatively modified proteins increased. When using the melanin preparation, in rats with cut wounds, there was a partial restoration of protein modification indicators.
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