CONTENT OF BIOACTIVE SUBSTANCES AND ELEMENTAL COMPOSITION OF SAINT JOHN’S WORT HERBS OF DIFFERENT PRODUCERS

Authors

DOI:

https://doi.org/10.11603/2312-0967.2018.4.9576

Keywords:

Hypericum perforatum, herb, St. John's wort, elemental composition, hypericin, rutin, hyperoside, atomic absorption spectrometry, high-performance liquid chromatography

Abstract

The aim of the work is to determine the content of bioactive substances and elemental composition of St. John's wort herbs of different producers and reveal correlations between the contents of API and microelements.

Materials and Methods. The research used certified reference materials for organic substances produced by the company Fluka and for metal ions produced by O.V.Bogatsky Physicochemical Institute, and samples of herbs of St. John's wort of different producers.

Analytical equipment. Hewlett Packard 8452A spectrophotometer; Shimadzu LC-20 liquid chromatograph, equipped with UV detector and Phenomenex Luna C18 column; and Solaar S4 AA atomic absorption spectrophotometer (Thermo Electron Co., USA).

Statistical calculations were performed using the IBM SPSS-19 statistical software package.

Results and Discussion. The content of bioactive substances (hypericin, hyperoside, rutin, quercetin, and chlorogenic, neochlorogenic, rosemary and caffeic acids) and elemental composition have been investigated for samples of St. John's wort herb of four different producers. Significant variation in the content of organic compounds is registered. For example, the content of hypericin, one of the main active pharmaceutical ingredients of St. John's wort, varies 2-5 times. Statistically significant positive or negative correlations were found between the contents of certain elements (K, Mg, Fe, Mn, and Cd) and the content of organic substances (rutin, hyperoside, hypericin and hydroxycinnamic acids).

Conclusions. The content of biologically active substances, namely hypericin, rutin, hyperoside and studied hydroxycinnamic acids, varies considerably depending on the origin and elemental composition of St. John's wort herb samples.

Author Biographies

  • T. M. Derkach, Kyiv National University of Technologies and Design

    associate professor of the Industrial Pharmacy Department, Kyiv National University of Technologies and Design, Ed.D. (habil.), PhD in Chemistry, associate professor

  • V. V. Strashnyi, Kyiv National University of Technologies and Design

    Chair of Industrial Pharmacy Department, Honored Worker of Science and Technology of Ukraine, Doctor of Pharmaceutical Sciences, Professor

  • O. O. Starikova, Kyiv National University of Technologies and Design

    Master`s degree of the Industrial Pharmacy Department

  • S. M. Lysenko, Private Joint Stock Company "Technologist"

    Chemist of the Private Joint Stock Company "Technologist"

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Published

2018-12-11

Issue

No. 4 (2018)

Section

Phytochemical researches

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How to Cite

CONTENT OF BIOACTIVE SUBSTANCES AND ELEMENTAL COMPOSITION OF SAINT JOHN’S WORT HERBS OF DIFFERENT PRODUCERS. (2018). Pharmaceutical Review Farmacevtičnij časopis, 4, 5-13. https://doi.org/10.11603/2312-0967.2018.4.9576