DEVELOPMENT OF SPECTROPHOTOMETRIC METHOD OF FLAVONOIDS DETERMINATION IN BILBERRY SHOOTS
DOI:
https://doi.org/10.11603/2312-0967.2018.4.9703Keywords:
bilberry (Vaccinium myrtillus L.), shoots, flavonoids, spectrophotometry, quantitative determination, contents, quality criterionAbstract
The aim of the work was to develop a spectrophotometric method of flavonoids determination in bilberries shoots.
Materials and Methods. The samples of bilberries shoots, collected in Transcarpathian, Ivano-Frankivsk, Ternopil and Volyn regions in 2014-2018, as well as the samples of the drug Shoots of Bilberries (PJSC Liktravy, Ukraine) were used in the study. The determination of flavonoids content was carried out by absorption spectrometry, measuring the absorption of flavonoids complex with aluminum chloride. The spectrophotometer Cary 50 UV-Vis (Agilent Technologies) was used to measure the absorption and record the electronic absorption spectra.
Results and Discussion. Electronic absorption spectra, which were obtained for the test solutions and the standard rutin solution in the conditions of complexation of flavonoids with aluminum chloride, were identical in the course of light absorption curves and were characterized by absorption maximum at 410±2 nm and 412±2 nm respectively that made it possible to make rutin a standard for recalculation of flavonoids content in the investigated plant raw material. When investigating the ethanol concentration effect on flavonoids extraction, it has been established that 70% (v / v) of ethanol should be used to prepare the sample solution. The flavonoids removal for an analytical purpose should be performed by heating under a reflux condenser three times – alternately together with 60, 20 and 15 ml of ethanol for 30, 15 and 15 minutes, respectively. An important aspect of the technique is the preparation of raw materials: a fraction (355) of crushed raw material should be used for analysis, given its content in the calculation formula. It has been established that the flavonoids content in the studied wild samples is within the range of 1.14-1.86%, and in the industrial series (except of raw materials of the series 20614) – 0.7-1.0%.
Conclusions. The spectrophotometric method of flavonoids quantitative determination in the bilberries (Vaccinium myrtillus L.) shoots was developed. Quantitative determination of the flavonoids content in 17 samples of raw materials of different origin was carried out as well. To evaluate the bilberry shoots quality, it was suggested to introduce an additional index ‘flavonoids content’ with a criterion – not less than 0,6% of flavonoids equivalent to rutin and dry raw material.
References
State Register of Ukraine Drugs. [Electronic resources]. – Available from: http://www.drlz.kiev.ua
Wing-kwan Chu, Sabrina C M Cheung, Roxanna AW Lau, Iris FF Benzie. Herbal Medicine: Biomolecular and Clinical Aspects. Chapter 4. Bilberry (Vaccinium myrtillus L.). Boca Raton (FL): CRC Press; 2011. Available from: http://www.ncbi.nlm.nih.gov/books/NBK92770/#ch4_51#ch4_51.
Ryazanova TK, Kurkin VA. New approaches to the integrated use of blackberry fruits and shoots. Izvest Samarskogo nauchn tsentra RAN. 2012;5(3):754-7. Russian
Nybakken Line, Selas Vidar, Ohlson Mikael. Increased growth and phenolic compounds in bilberry (Vaccinium myrtillus L.) following forest clear-cutting. Scandinavian J of Forest Research. 2013;28(4):319-30.
Ryazanova TK. Pharmacognostic study of the fruits and shoots of bilberry. Fundamental'nyye issledovaniya. 2013;8:1136-40. Russian
Vronska LV, Chubka MB, Demyd AYe. Development of phenolic compounds chromatographic identification in bilberry shoots. Farmats chasopys. 2015;3:28-33.
Mechnikova GYa, Matyushenko NV, Smirnova OV. Validation of the method of quantitative determination of the flavonoids amount in the shoots Vaccinium myrtillus L. Bashkirskiy khimicheskiy zhurnal. 2013;20(2):66-71. Russian
Petrov EV. Development of method for the quantitative determination of the flavonoids content in the shoots of bilberry. Byulleten' VSNTS SO RAMN. 2010;3(73):253-5. Russian
Yakovlev GP, Blinova KF. Medicinal plant materials. Pharmacognosy: studies. allowance [Лекарственное растительное сырье. Фармакогнозия: учеб. пособие] SPb : SpetsLit; 2004. Russian
Kovalev VM, Pavly OI, Isakova TI. Pharmacognosy with the basics of biochemistry of plants [Фармакогнозія з основами біохімії рослин] Kharkiv : Prapor , 2000. Ukrainian.
State Pharmacopoeia of Ukraine. 2nd ed. Kharkiv: State Enterprise Ukrainian Scientific Pharmacopoeia Center for the Quality of Medicines; 2018. Ukrainian.
Kurylo H I, Klishch IM, Volska AS, Barchuk OZ. Investigation of hypoglycemic properties of dry bilberry extract. Medychna ta klinichna khimiya. 2016;2:38-41. Ukrainian.
Kurkin VA, Ryazanova TK. Quantitative determination of the flavonoids sum in the bilberries. Khym farmats zhurn. 2013;47(4):34-7.
Downloads
Published
Issue
Section
License
The authors who publish their materials in this journal agree with the following terms:
- Authors reserve the right to authorship of their work and assign to the journal the right to first publish this work under the terms of the Creative Commons Attribution License, which allows other persons to freely distribute the published work with a mandatory reference to the authors of original work and the first publication of work in this journal
. - Authors have the right to make independent extra-exclusive work agreements in the form they are published by this journal (for example, posting work in an electronic repository of an institution or publishing as part of a monograph), provided that the link to the first publication of the work in this journal is maintained.
Journal policy allows and encourages publication of manuscripts on the Internet (for example, in institutions repositories or on personal websites), both before the publication of this manuscript and during its editorial review, as it contributes to productive scientific discussion and positively affects the efficiency and dynamics of the citation of the published work (see The Effect of Open Access).
