THE FREQUENCY OF SEROPOSITIVE INDIVIDUALS TO THE COMPLEX B. BURGDORFERI SENSU LATO AMONG FORESTRY WORKERS OF ZHYTOMYR, KHMELNYTSKYI AND VOLYN REGION

Authors

  • M. I. Shkilna I. Horbachevsky Ternopil State Medical University
  • M. A. Andreychyn I. Horbachevsky Ternopil State Medical University
  • M. M. Korda I. Horbachevsky Ternopil State Medical University
  • I. M. Klishch I. Horbachevsky Ternopil State Medical University
  • S. Yo. Zaporozhan I. Horbachevsky Ternopil State Medical University
  • R. S. Morochkovskyi Volyn regional infection hospital

DOI:

https://doi.org/10.11603/1681-2727.2018.3.9408

Keywords:

Lyme borreliosis, ELISA, immunoblot, antibodies, antigens western blot, forestry workers, Zhytomyr, Khmelnytskyi and Volyn region

Abstract

The aim of the study was to learn the prevalence of Lyme borreliosis among forestry workers of Zhytomyr, Khmelnytskyi and Volyn region, using a two-stage diagnostic scheme: ELISA and immunoblot.

Patients and Methods. The present studies were conducted based on 397 forestry workers of Zhytomyr, Khmelnytskyi and Volyn region. The age of tested persons ranged from 20-65 years. The group of examined workers comprised 339 men and 98 women. A two-step algorithm consisting of ELISA and immunoblot tests was used in the study. During the first phase of the tests, presence of anti-Borrelia burgdorferi s.l IgM/IgG antibodies were marked using ELISA method, and positive and uncertain results were confirmed by immunoblot (EUROLINE Borrelia RN-AT). The persons tested were also questioned about Lyme-borreliosis, using international questionnaire.

Results. In 190 out 397 the examined forestry workers (47.9 %) from Zhytomyr, Khmelnytskyi and Volyn region, presence of specific antibodies: at least one IgM and / or IgG class to B. burgdorferi sensu lato (B. burgdorferi sensu stricto, B. afzelii and B. garinii) were detected by ELISA test. The application of the immunoblot method (EUROLINE Borrelia RN-AT) allowed to detect specific antibodies: at least one IgM and / or IgG class to B. burgdorferi sensu lato in 171 (90.0 %) of respondents, which had positive results during the first phase of the tests (ELISA).

Conclusions. The prevalence of Lyme borreliosis in forestry workers of Zhytomyr, Khmelnytskyi and Volyn region is 47.9 % during the first phase of the tests (ELISA) and 49.3 % during immunoblot (EUROLINE Borrelia RN-AT) laboratory diagnostic tests for Lyme disease must be performed in accordance with the current standards, positive and uncertain results must be confirmed by immunoblot test. Such percentage of infection by the B. burgdorferi sensu lato complex of surveyed forestry workers in Zhytomyr, Khmelnytskyi and Volyn region makes it possible to consider forestry workers as a high-risk group in relation to the Lyme disease and to achieve official recognition of Lyme disease as an occupational disease in Ukraine. A low percentage (3.3 %) of appeals for medical help of forest workers in the tested regions of Ukraine is in need of developed and implemented educational, prevention and efficient monitoring standards.

Author Biographies

  • M. I. Shkilna, I. Horbachevsky Ternopil State Medical University

    Candidate of Medicine, Associate Professor of the Infectious Diseases and Epidemiology, Skin and Venereal Illnesses Department of I. Horbachevsky Ternopil State Medical University

  • M. A. Andreychyn, I. Horbachevsky Ternopil State Medical University

    Academician of the NAMS, Doctor of Medicine, Professor, Head of the Infectious Diseases and Epidemiology, Skin and Venereal Illnesses Department of I. Horbachevsky Ternopil State Medical University

  • M. M. Korda, I. Horbachevsky Ternopil State Medical University

    Doctor of Medicine, Professor of the Department of Medical Biochemistry of I. Horbachevsky Ternopil State Medical University

  • I. M. Klishch, I. Horbachevsky Ternopil State Medical University

    Doctor of Biological Sciences, Professor of the Department of Functional and Laboratory Diagnostics of I. Horbachevsky Ternopil State Medical University

  • S. Yo. Zaporozhan, I. Horbachevsky Ternopil State Medical University

    Doctor of Medicine, Professor of the Department of General Surgery of I. Horbachevsky Ternopil State Medical University

  • R. S. Morochkovskyi, Volyn regional infection hospital

    Candidate of Medicine, Chief Doctor of Volyn Regional Infectious Hospital

References

Andreichyn, M.A. (Ed.). (2007). Infektsiini khvoroby u zahalnii praktytsi ta simeinii medytsyni [Infectious diseases in general practice and family medicine]. Ternopil: TDMU, Ukrmedknyha [in Ukrainian].

Svinitskyi, A.S. (2012). Laimska khvoroba yak aktualna intehrovana problema suchasnoi vnutrishnoi medytsyny [Lyme disease as an actual integrated problem of modern internal medicine]. Zdorova Ukrainy – Health of Ukraine, 5 (25), 84-87 [in Ukrainian].

Halperin, J.J. (2014). Nervous system Lyme disease. Handb. Clin. Neurol., 121, 1473-1483.

Stanek, G., Lusa, L., Ogrinc, K., Markowicz, M., Strle, F. (2014). Intrathecally produced IgG and IgM antibodies to recombinant VlsE, VlsE peptide, recombinant OspC and whole cell extracts in the diagnosis of Lyme neuroborreliosis. Med. Microbiol. Immunol., 203(2), 125-132.

CDC. (1995). Recommendations for test performance and interpretation from the second national conference on serologic diagnosis of Lyme disease.

Robertson, E., Guy, N., Andrews, B., Wilske, P., Anda, M., Granström G., … Gray, J. (2000). A European multicenter study of immunobloting in serodiagnosis of Lyme borreliosis. J. Clin. Microbiol., 38, 2097-2102.

Buczek, A., Rudek, A., Bartosik, K., Szymanska, J., Wojcik-Fatla, A. (2009). Seroepidemiological study of Lyme borreliosis among forestry workers in southern Poland. Ann. Agric. Envіron Med., (16), 257-261.

Thorin, C., Rigaud, E., Capek, I., Andre-Fontaine, G., Oster, B., Gastinger, G., Abadia, G. (2008). Seroprevalence of Lyme borreliosis and tick-borne encephalitis in workers at risk, in Eastern France. Med Mal Infect., 38(10), 533-542.

Kaya, A.D., Parlak, A.H., Ozturk, C.E., Behcet1 М. (2008). Seroprevalence of Borrelia burgdorferi infection among forestry workers and farmers in Duzce, north-western Turkey. New Microbiol., 31 (2), 203-209.

Wуjcik-Falta, A., Szymaсska, J., Buczek, A., Buczek, A., Rudek, A. (2009). Diseases transmitted by ticks. Pathogens Borrelia burgdorferi, Anaplasma phagocytophilum, Babesia microti. Public Health., 119 (2), 217-222.

Shkilna, M.I. (2016). Laim-borelioz u pratsivnykiv lisovykh hospodarstv Ternopilskoi oblasti [Lyme-borreliosis in forestry workers of Ternopil region]. Infektsiini khvoroby – Infectious Diseases, 1 (83), 36-40 [in Ukrainian].

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Published

2018-10-11

Issue

No. 3 (2018)

Section

Original investigations

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How to Cite

THE FREQUENCY OF SEROPOSITIVE INDIVIDUALS TO THE COMPLEX B. BURGDORFERI SENSU LATO AMONG FORESTRY WORKERS OF ZHYTOMYR, KHMELNYTSKYI AND VOLYN REGION. (2018). Infectious Diseases – Infektsiyni Khvoroby, 3, 18-25. https://doi.org/10.11603/1681-2727.2018.3.9408