LIPOGENESIS IN ADIPOSE TISSUE OF THE LABORATORY ANIMALS AFTER LOADING WITH CHOLESTEROL

Abstract

The intensity of synthesis of different lipids classes was studied in vitro (fatty acids, cholesterol, phospholipids and acylglycerols) in the homogenates of the adipose tissue after a single daily loading of rats and guinea pigs with the cholesterol (300 mg/kg body weight) during 30 days period. For this purpose, the radioactivity of lipid fractions in the homogenates of adipose tissue of the rats and guinea pigs was measured, while homogenates were incubated separately with [6-¹⁴C] glucose, [2-¹⁴C] lysine or [1-¹⁴C] palmitic acid. The inhibitory effect of cholesterol was established by increasing its level in the diet of rats and guinea pigs on all classes of lipid synthesis in adipose tissue in vitro when [6- ¹⁴C] glucose and [2- ¹⁴C] lysine were used as precursors, and on the synthesis of cholesterol when [1-¹⁴C] palmitic acid was used as precursor.