CHANGES OF HEMOPOIESIS IN WISTAR HAN RATS BONE MARROW AND SPLEEN AS A MECHANISM OF HEMATOTOXIC ACTION OF TEBUCONAZOLE
Introduction. One of the most commonly used active substances of the triazole fungicides group is tebuconazole. Preliminary toxicological studies of peripheral blood have shown hematotoxic effect.
The aimof the study– to investigate the hemopoiesis in the bone marrow and spleen of Wistar Han male rats after acute intoxication with technical tebuconazole, 97%.
ResearchMethods. 15 adult Wistar Han male rats were divided into control (0 mg / kg) and 2 experimental groups.Toxic dose of tebuconazole 1700 mg/kg/bw (1/2 of LD50) was administered once orally by gavage. Peripheral blood studies were performed at 0, 1, 3, 7 and 14 postexposure days (PED). Quantitative amount of erythrocytes and reticulocytes, hemoglobin concentration, hematocrit level, erythrocytic indices, number of leukocytes and platelets were examined. Hemogram with morphological assessmentof cells were analyzed in peripheral blood smears. Studies of hematopoiesis in the bone marrow (myelogram) and in the spleen (splenogram) were performed at 7 and 14 PED.
Results and Discussion. Anemic effect, reticulocytopenia and the appearance of macrophages in peripheral blood have been established. Depression of erythropoiesis in the bone marrow with a violation of hemoglobin formation in normocytes and the development of extramedullary hemopoiesis in the spleen have been observed. A suppressive action on eosinophilic and basophilic cell types has been defined. Compensatory activation of neutrophilopoiesis, lymphocytopoiesis and monocytopoiesis in bone marrow was shown.
Conclusion. Several mechanisms of hematotoxic action of technical tebuconazole, 97%: hemoglobin formation impairment, erythropoiesis depression with pathological regeneration, development of extramedular hemopoiesis in the spleen and influence on some parts of immunity have been established.
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