THE EFFECT OF IOSITOL ON THE DEVELOPMENT OF NITRO-OXIDATIVE STRESS IN THE LIVER AND SKIN OF DIFFERENT SEXES RATS WITH STEATOHEPATOSIS
DOI:
https://doi.org/10.11603/mcch.2410-681X.2023.i4.14381Keywords:
nitrite anion, liver, skin, fructose, myo-inositol, D-chiro inositol, steatohepatosis, rats, genderAbstract
Introduction. Steatohepatosis is one of the main problems today. One of the methods of correcting liver damage can be the use of inositol preparations.
The aim of the study – to evaluate changes in the content of nitrite anion in the liver and skin of different sexes rats with steatohepatosis and the effect of inositol preparations on it.
Research Methods. Experiments were performed on 216 white rats of different sexes, at the time of withdrawal from the experiment they were 7.5–8 months old. The animals were divided into 9 groups: 1 – control, 2 – myo-inositol (MI), 3 – D-chiro inositol (DI), 4 – steatohepatosis 2 months, 5 – steatohepatosis 2 months + MI 2 months, 6 – steatohepatosis 2 months + DI 2 months, 7 – steatohepatosis 4 months, 8 – steatohepatosis 4 months + MI 2 last months, 9 – steatohepatosis 4 months + DI 2 last months. Steatohepatosis was induced by drinking of 20 % fructose solution (Fr) for 2 months in groups 4, 5 and 6, and for 4 months in groups 7, 8 and 9. Inositols were administered intragastrically (MI – 400 mg/kg of weight, DI – 30 mg/kg of weight) 2 months after the beginning of steatohepatosis simulation. The content of nitrite anion was determined in liver and skin homogenates.
Results and Discussion. In group 1 of rats, the content of nitrite anion in the liver was higher by 45.1 % (p<0.001) and in the skin – by 15.8 % (p<0.001) in females. MI caused a significant increase in the content of nitrite anion in the liver of males by 12.4 % (p<0.001), and DI – by 3.5 % (p<0.05). In the liver and skin, the higher content of nitrite anion was maintained in females when MI or DI was administered. In rats with striatohepatosis, an increase in the content of nitrite anion in the liver was noted, which depended on the duration of use of Fr. The same pattern was found in the skin of females. The effect of Fr on the liver, compared to the skin, regarding the content of nitrite anion was greater. A higher content of nitrite anion was noted in the liver and skin of males only after 2 months. After 4 months, the values in males and females were not significantly different in the liver, and in the skin were higher in females. The introduction of MI or DI contributed to a reliable reduction of nitrite anion in the liver and skin, but the return to control values was only in the skin of females of groups 5 and 6. When the use of Fr DI was stopped, it had a better effect on the liver of females. A significant decrease in nitrite anion content was noted in the liver and skin of males and females that continued to be given Fr and were simultaneously injected with MI or DI. In the skin of animals of groups 8 and 9, compared to 5 and 6, the content of nitrite anion was lower in males, and higher in females.
Conclusion. Steatohepatosis in the liver and skin of rats of different sexes causes the development of nitrosidative stress. In the liver of animals, it directly depends on the duration of use of Fr, and in the skin, such a dependence is noted only in females, in males it is smaller after 4 months of drinking Fr. MI and DI cause a decrease in the content of nitrite anion in the liver and skin of rats. When stopping the use of Fr MI and DI have a more pronounced effect on the liver and skin of females.
References
Wu, W., Ma, W., Yuan, S., Feng, A., Li, L., Zheng, H., et al. (2023). Associations of Unhealthy Lifestyle and Nonalcoholic Fatty Liver Disease With Cardiovascular Healthy Outcomes. J. Am. Heart. Assoc., 12(23). https://doi.org/10.1161/JAHA.123.031440.
Younossi, Z., Anstee, Q.M., Marietti, M., Hardy, T., Henry, L., Eslam, M., et al. (2018). Global burden of NAFLD and NASH: Trends, predictions, risk factors and prevention. Nat. Rev. Gastroenterol. Hepatol., 15. https://doi.org/10.3390/nu12113379.
Pani, A., Giossi, R., Menichelli, D., Fittipaldo, V.A., Agnelli, F., Inglese, E., et al. (2020). Inositol and Non-Alcoholic Fatty Liver Disease: A Systematic Review on Deficiencies and Supplementation. Nutrients, 12(11). https://doi.org/10.3390/nu12113379.
Wu, W., Zhang, Z., Qi, Y., Zhang, H., Zhao, Y., & Li, J. (2023). Association between dietary inflammation index and hypertension in participants with different degrees of liver steatosis. Ann. Med., 55(1). https://doi.org/10.1080/07853890.2023.2195203.
Huang, Y.C., Huang, J.C., Chien, H.H., Lin, C.I., Chuang, Y.S., Cheng, H.Y., et al. (2023). Performance of nonalcoholic fatty liver fibrosis score in estimating atherosclerotic cardiovascular disease risk. Nutr. Metab. Cardiovasc. Dis., 33(12). https://doi.org/10.1016/j.numecd.2023.08.005.
Tabaeian, S.P., Rezapour, A., Azari, S., Martini, M., Saran, M., Behzadifar, M., et al. (2024). Prevalence of Non-alcoholic Fatty Liver Disease in Iran: A Systematic Review and Meta-analysis. J. Clin. Exp. Hepatol., 14(1). https://doi.org/10.1016/j.jceh.2023.06.009.
Tran, S., Zou, B., Kam, L., Lee, K., Huang, D. Q., Henry, L., et al. (2023). Updates in Characteristics and Survival Rates of Hepatocellular Carcinoma in a Nationwide Cohort of Real-World US Patients, 2003-2021. J. Hepatocell. Carcinoma, 10. https://doi.org/10.2147/JHC.S420603.
Gagnon, E., Paulin, A., Mitchell, P.L., & Arsenault, B.J. (2023). Disentangling the impact of gluteofemoral versus visceral fat accumulation on cardiometabolic health using sex-stratified Mendelian randomization. Atherosclerosis, 386. https://doi.org/10.1016/j.atherosclerosis.2023.117371.
Anstee, Q.M., Mantovani, A., Tilg, H., & Targher, G. (2018). Risk of cardiomyopathy and cardiac arrhythmias in patients with nonalcoholic fatty liver disease. Nat. Rev. Gastroenterol. Hepatol., 15. https://doi.org/10.1038/s41575-018-0010-0.
Zhang, H.F., Zhang, Q.X., Feng, Y.H., Li, S.J., & Xie, B.Y. (2023). Association of thyroid disease and risk of fatty liver disease: an exposure-wide Mendelian randomization study. Eur. Rev. Med. Pharmacol. Sci., 27 (23). https://doi.org/10.26355/eurrev_202312_34579.
Kiani, A.K., Paolacci, S., Calogero, A.E., Cannarella, R., Di Renzo, G.C., Gerli, S., et al. (2021). From Myo-inositol to D-chiro-inositol molecular pathways. Eur. Rev. Med. Pharmacol. Sci., 25(5). https://doi.org/10.26355/eurrev_202103_25279.
Kostiuk, O.A., Denefil, O.V., & Holovata, T.K. (2018). Changes in biochemical parameters in the blood of high- and low-emotional rats with ethanol hepatosis. Medical and Clinical Chemistry, 20 (3). https://doi.org/10.11603/mcch.2410-681X.2018.v0.i3.9578 [in Ukrainian].
Bevilacqua, A., Dragotto, J., Giuliani, A., Bizzarri, M. (2019). Myo-inositol and D-chiro-inositol (40:1) reverse histological and functional features of polycystic ovary syndrome in a mouse model. J. Cell Physiol., 234. https://doi.org/10.1002/jcp.27623
Sklyarov, O.Ya., Fedorovych, I.P., Korobov, V.M., Fartushok, N.V., Fedevich, Yu.M., & Kolinkovskiy O.M. (2004). Changes in the concentration of NO2¯ in biological fluids in gastric cancer. Medical Chemistry, 6(3).
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Medical and Clinical Chemistry
This work is licensed under a Creative Commons Attribution 4.0 International License.
