THE EFFECT OF IOSITOL ON THE DEVELOPMENT OF NITRO-OXIDATIVE STRESS IN THE LIVER AND SKIN OF DIFFERENT SEXES RATS WITH STEATOHEPATOSIS

Authors

  • O. V. Sopel I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • O. V. Denefil I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY

DOI:

https://doi.org/10.11603/mcch.2410-681X.2023.i4.14381

Keywords:

nitrite anion, liver, skin, fructose, myo-inositol, D-chiro inositol, steatohepatosis, rats, gender

Abstract

Introduction. Steatohepatosis is one of the main problems today. One of the methods of correcting liver damage can be the use of inositol preparations.

The aim of the study – to evaluate changes in the content of nitrite anion in the liver and skin of different sexes rats with steatohepatosis and the effect of inositol preparations on it.

Research Methods. Experiments were performed on 216 white rats of different sexes, at the time of withdrawal from the experiment they were 7.5–8 months old. The animals were divided into 9 groups: 1 – control, 2 – myo-inositol (MI), 3 – D-chiro inositol (DI), 4 – steatohepatosis 2 months, 5 – steatohepatosis 2 months + MI 2 months, 6 – steatohepatosis 2 months + DI 2 months, 7 – steatohepatosis 4 months, 8 – steatohepatosis 4 months + MI 2 last months, 9 – steatohepatosis 4 months + DI 2 last months. Steatohepatosis was induced by drinking of 20 % fructose solution (Fr) for 2 months in groups 4, 5 and 6, and for 4 months in groups 7, 8 and 9. Inositols were administered intragastrically (MI – 400 mg/kg of weight, DI – 30 mg/kg of weight) 2 months after the beginning of steatohepatosis simulation. The content of nitrite anion was determined in liver and skin homogenates.

Results and Discussion. In group 1 of rats, the content of nitrite anion in the liver was higher by 45.1 % (p<0.001) and in the skin – by 15.8 % (p<0.001) in females. MI caused a significant increase in the content of nitrite anion in the liver of males by 12.4 % (p<0.001), and DI – by 3.5 % (p<0.05). In the liver and skin, the higher content of nitrite anion was maintained in females when MI or DI was administered. In rats with striatohepatosis, an increase in the content of nitrite anion in the liver was noted, which depended on the duration of use of Fr. The same pattern was found in the skin of females. The effect of Fr on the liver, compared to the skin, regarding the content of nitrite anion was greater. A higher content of nitrite anion was noted in the liver and skin of males only after 2 months. After 4 months, the values in males and females were not significantly different in the liver, and in the skin were higher in females. The introduction of MI or DI contributed to a reliable reduction of nitrite anion in the liver and skin, but the return to control values was only in the skin of females of groups 5 and 6. When the use of Fr DI was stopped, it had a better effect on the liver of females. A significant decrease in nitrite anion content was noted in the liver and skin of males and females that continued to be given Fr and were simultaneously injected with MI or DI. In the skin of animals of groups 8 and 9, compared to 5 and 6, the content of nitrite anion was lower in males, and higher in females.

Conclusion. Steatohepatosis in the liver and skin of rats of different sexes causes the development of nitrosidative stress. In the liver of animals, it directly depends on the duration of use of Fr, and in the skin, such a dependence is noted only in females, in males it is smaller after 4 months of drinking Fr. MI and DI cause a decrease in the content of nitrite anion in the liver and skin of rats. When stopping the use of Fr MI and DI have a more pronounced effect on the liver and skin of females.

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Published

2024-01-09

How to Cite

Sopel, O. V., & Denefil, O. V. (2024). THE EFFECT OF IOSITOL ON THE DEVELOPMENT OF NITRO-OXIDATIVE STRESS IN THE LIVER AND SKIN OF DIFFERENT SEXES RATS WITH STEATOHEPATOSIS. Medical and Clinical Chemistry, (4), 107–112. https://doi.org/10.11603/mcch.2410-681X.2023.i4.14381

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Section

ORIGINAL INVESTIGATIONS