EXPERIMENTAL RESEARCH ON THE DEVELOPMENT OF ANALYSIS METHODS OF BIOLOGICALLY ACTIVE SUBSTANCES IN A MULTI-COMPONENT LIQUID EXTRACT OF COMPLEX ACTION

Abstract

Introduction. Diseases of the digestive organs are one of the most frequent causes of loss of working capacity and development of disability. According to literature sources, the cause of disability in every seventh patient is the pathology of the digestive organs. The economic damage from these diseases is twice as high as that from cardiovascular diseases.

The aim of the study – to conduct experimental studies on the development of methods for the analysis of biologically active substances (BAS) in a liquid multicomponent extract.

Research Methods. The objects of the research are experimental samples of liquid multicomponent extract. To identify BAS in the studied extract, the method of thin-layer chromatography (TLC) was used, as the stationary phase, thin-layer Silica gel 60 plates from Merk were used. The quantitative content of the main BAS in the extract was determined by absorption spectrophotometry using a UV-2600 spectrophotometer, Japan.

Results and Discussion. The studied extract consists of 10 plants that contain a significant amount of biolo-
gically active compounds of the flavonoid structure, including the pot marigold flowers, common horsetail grass, horse-heal rhizomes, heath speedwell grass, common chicory roots, and garden parsley fruits. The rhizomes of tormentils are rich in tannins, the fruits of common fennel contain essential oil, and shepherd's purse contains compounds of a polyphenolic structure. By the method of thin-layer chromatography, in comparison with marker substances, the presence of flavonoid substances similar in structure to luteolin and rutin was proven in the multicomponent liquid extract; acids of the polyphenolic structure, similar in structure to chlorogenic acid; substances similar in structure to tannins (catechin) and polysaccharides (fructose). Techniques have been developed to determine the quantitative content of flavonoid substances in plant liquid extract, in terms of luteolin (not less than 0.22 %) – by spectrophotometric method; substances of the polyphenolic structure, in terms of gallic acid (not less than 1.04 %) – by the spectrophotometric method; the content of phenolic compounds, in terms of pyrogallol (not less than 0.032 %) – by the spectrophotometric method.

Conclusions. As a result of experimental studies, methods of identification and quantitative determination of the content of the main biologically active substances in a multicomponent liquid extract were developed.