SPECTROPHOTOMETRIC DETERMINATION OF FLAVONOIDS IN INFLORESCENCE OF THE “SUPER HERO ORANGE FLAME” BRAND OF TAGETES PATULA L
DOI:
https://doi.org/10.11603/mcch.2410-681X.2026.i2.15747Keywords:
Tagetes patula L.; brand “Super Hero Orange Flame”; flavonoids.Abstract
Introduction. Cultivating plants from the genus Tagetes (Marigold) for decorative purposes and as a source of bioactive compounds is a popular practice in Ukraine. Among them, T. erecta L. (Marigold erect), T. patula L. (Marigold spreading), and T. tenuifolia L. (narrow-leaved Marigold) are the most widely distributed. Although the experimental data on the composition of bioactive compounds in these plants are rather substantial, information related to new varieties of T. patula L., such as “Super Hero Orange Flame” by “Benary”, Germany, is still scarce. As found in our previous investigations, this variety reveals a high content of flavonoids. The aim of the article – identification and quantification of the content of flavonoids in the inflorescence of the variety “Super Hero Orange Flame” of Tagetes рatula L. Methods. This investigation was conducted on the air-dry inflorescences of T. рatula L., variety “Super Hero Orange Flame”. Identification and quantification of flavonoids were performed using spectrophotometry. Results and Discussion. The results of spectral investigation of ethanolic extracts from marigold flowers and aqueous extracts from the inflorescences revealed that this material consists of a mixture of different phenols, including flavonoids. A 70 % ethanol solution proved to be the most effective for extraction. The total flavonoid content in terms of hyperoside was 1.492 ± 0,026%. Conclusions. 1. An adapted method for quantitative determination of the amount of flavonoids, expressed as hyperoside, in inflorescences of Tagetes patula L., variety "Super hero Orange Flame" is valid within the linearity range. 2. The presence of flavonoids in inflorescences of Tagetes patula L., variety "Super hero Orange Flame" collected during the period of blooming has been confirmed using the method of spectrophotometry. 3. The content of flavonoids in inflorescences of Tagetes patula L., variety "Super Hero Orange Flame" is 1.492 ± 0.026% expressed as hyperoside in terms of dry components.
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