INFLUENCE OF EXPERIMENTAL MATERNAL HYPO- AND HYPERTHYROIDISM ON THE LECTIN PROFILE OF PROGENY ADRENAL GLANDS
DOI:
https://doi.org/10.11603/2415-8798.2018.4.9626Keywords:
rats, ontogenesis, adrenal glands, thyroid gland, maternal hypo- and hyperthyroidismAbstract
By means of lectin histochemistry methods, the influence of experimental hypo- and hyperthyroidism of maternal organism on the structural components of progeny adrenal glands has been investigated on the successive stages of pre- and postnatal ontogenesis.
The aim of the study – to expand the spectrum of known carbohydrate determinants to obtain a more complete picture of glycemic adrenal gland changes that developed under conditions of simulated experimental pathology.
Material and Methods. Hypothyroidism was induced by daily feeding of female rats with 10 mg/kg of anti-thyroid drug Mercacolyl, and hyperthyroidism was induced by feeding L-thyroxine at a dose of 100 mkg/kg. The lectin panel included concanavalin A (Con A), Lens culinaris agglutinin (LCA), Narcissus pseudonarcissus agglutinin (NPA), peanut agglutinin (PNA), Helix pomatia agglutinin (HPA), Clitocybe nebularis fungus agglutinin (CNFA), wheat germ agglutinin (WGA), Sambucus nigra agglutinin (SNA), Laburnum anagyroides bark agglutinin (LABA).
Results and Discussion. It was estimated that the morphogenesis of rat adrenal glands basically culminates on the 10th postnatal day and is manifested by the selective exposure of LCA, PNA, CNFA, WGA, SNA and LABA lectin receptor sites in the glandular medullary portion. Hypothyroidism of the maternal organism caused delayed maturation, dilation of medullary vasculature, swelling of the parenchyma in combination with leukocyte infiltration of zona reticularis and medullary portion, alterations in glycomic structure of progeny adrenal glands. Changes in the glycoreceptors induced by hypo- and hyperthyroidism were similar yet not identical, which may be due to the negative effect of both pathological conditions on the final steps of glycosylation. WGA can be used for differential detection of epinephrocytes and norepinephrocytes, apoptosomes in the adrenal gland medulla; PNA – for visualization of Golgi complex in within the adrenocorticocytes, and secretory granules of chromaffin cells; LCA and LABA – for the detection of Golgi complex in chromaffin cells; NPA and PNA – for selective labeling of activated macrophages; Con A and SNA – for labeling of migrant eosinophilic granulocytes, which under conditions of hypothyroidism? But not hyperthyroidism infiltrated stroma of the adrenal glands.
Conclusions. Current study showed a significant impact of thyroid hormones imbalance within the maternal organism on morphogenesis, micromorphology, and carbohydrate determinants of progeny adrenal glands, with hypothyroidism inducing more pronounced changes in comparison with hyperthyroidism. The obtained data on the possibilities of selective marking by lectins of the rat adrenal glands structural components may be useful for monitoring of physiological and pathological processes in these same organs.
References
Pankiv, V.I. (2011). Praktychna tyreoyidolohiya [Practical thyroidology]. Donetsk, Zaslavsky, 224 p [in Ukrainian].
Yashchenko, A.M., & Lutsyk, S.A. (2018). Vplyv hipo- ta hipertyreozu na morfohenez ta histofiziolohiyu nadnyrnykiv [The influence of hypo- and hyperthyroidism on morphogenesis and histophysiology of adrenal glands]. Zhurnal embriolohii ta doslidzhennia stovburovykh klityn – Journal of Embryology and Stem Cell Research, 2 (1), 000107 [in Ukrainian].
Chung, H.R. (2014). Adrenal and thyroid function in the fetus and preterm infant. Korean J. Pediatr, 57 (10), 425-433.
Gabius, H.J. (2018). The sugar code: why glycans are so important. Biosystems, 164, 102-111.
Hirabayashi, J. (Ed.) (2014). Lectins. Methods and protocols. Methods in molecular biology 1200. New-York, Springer, p. 614.
Pellicciari, C., & Biggiogera, M. (Eds.) (2017). Histochemistry of single molecules. Methods and protocols. Methods in molecular biology (vol. 1560. Part II.). Lectin histochemistry. Springer, 93-147.
Roth, J. (2011). Lectins for histochemical demonstration of glycans. Histochem Cell Biol, 136 (2), 117-130.
Antonyuk, V.A. (2005). Lektyny ta yikhni resursy [Lectins and their resources]. Lviv, Kvart, p. 554 [in Ukrainian].
Sharon, N., & Lis, H. (2007). Lectins. (2nd ed.). Dordrecht, Springer, p. 464.
Varki, A., Cummings, R.D., Esko, J.D., Freeze, H.H., Stanley, P., Bertozzi, C.R., … & Etzler, M.E. (2009). Essentials of glycobiology. (2nd ed.) Cold Spring Harbor, Cold Spring Harbor Laboratory Press, 29-178.
Lutsyk, S.A., & Yashchenko, A.M. (2018). Histotopohrafiia saitiv WGA-retseptoriv v postnatalnomu morfohenezi nadnyrkovykh zaloz shchuriv u fiziolohichnykh umovakh ta pry eksperymentalnomu hipo- ta hipertyreozi materynskoho orhanizmu [Histotopography of WGA receptor sites in postnatal morphogenesis of rat adrenal gland in physiological conditions and under experimental hypo- and hyperthyroidism of maternal organism]. Visnyk problem biolohiyi ta medytsyny – Bulletin of Problems in Biology and Medicine, 2 (144), 325-329 [in Ukrainian].
Lutsyk, S.A., Strus, Ch.I., & Yashchenko, A.M. (2018). Histokhimiia i morfometrychni kharakterystyky lektynu nadnyrnykiv shchuriv, shcho rozvyvaiutsia pry hipo- ta hipertyreozi matky [Lectin histochemistry and morphometric characteristics of adrenal glands of the rats progeny developing under maternal hypo- and hyperthyroidism]. Morfolohiya – Morphology, 12 (2), 30-39 [in Ukrainian].
Ahi, M., Zamansoltani, F., Taheri, M.M.H., & Bideskan, A.R.E. (2007). The role of GalNAc terminal sugar on adrenal gland development. Adv Biol Res, 1 (1-2), 34-39.
Detiuk, E.S., & Avgustinovich, M.S. (1976). Оn the morphofunctional peculiarities of progeny adrenal glands developing under the influence of maternal hypothyroidism. Archiv of Anatomy, Histology and Embryology, 71 (10), 41-45.
Parker, G.A., & Picut, C.A. (Eds.) (2016). Atlas of histology of the juvenile rat. Amsterdam: Elsevier-Academic Press, 259-263, 275-291.
Sogomonian, E.A., & Lutsyk, A.D. (2010). Histological structure and lectin receptor sites redistribution in endometrium of rats under experimental hypo- and hyperthyroidism. Morphological Bulletin, 16(3), 558-564.
Lutsyk, A.D., Yaschenko, A.M., & Detuk, E.U. (1987). Binding of lectins to the structures of the submandibular salivary gland of rats in postnatal ontogenesis with thyroid pathology. Archive of Anatomy, Histology and Embryology, 92 (2), 40-48.
Johnson, E.O., Calogero, A.E., Konstandi, M., Kamilaris, T.C., La Vignera, S., & Chrousos, G.P. (2013). Effects of experimentally induced hyperthyroidism on central hypothalamic-pituitary-adrenal axis function in rats: in vitro and in situ studies. Pituitary, 16 (2), 275-286.
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