STUDY OF ANTI-INFLAMMATORY PROPERTIES OF EXTRACTS FROM THE ROOTS OF GENTIANA ASCLEPIADEA L.
DOI:
https://doi.org/10.11603/mcch.2410-681X.2023.i4.14283Keywords:
Gentiana asclepiadea L., liquid and thick extracts, anti-inflammatory activity, antiexudative activity, experimental animals, white outbred rats, non-steroidal anti-inflammatory drugsAbstract
Introduction. The leading place among drugs used to weaken the inflammatory and painful reaction of the body is occupied by non-steroidal anti-inflammatory drugs. But drugs of this group show serious side effects that limit their use. Today, the search and development of new drugs, including herbal origin, with anti-inflammatory and analgesic properties is carried out in order to potentiate therapeutic effects and increase their safety.
The aim of the study – to investigate the anti-inflammatory activity of thick and liquid extracts of Gentiana asсlepiadea roots.
Research Methods. The study of the anti-inflammatory activity of thick and liquid extracts of Gentiana asсlepiadea L. was carried out according to the methodological recommendations of the State Expert Center of the Ministry of Health of Ukraine on sexually mature white outbred rats weighing 180–220 g on a model of aseptic formalin inflammation. The study and analysis of the obtained results were carried out in comparison with the drug from the group of non-steroidal anti-inflammatory drugs – sodium diclofenac. The inflammatory response was assessed oncometrically by increasing the volume of rat paw, which was measured prior to administration of 2 % formalin solution, 1 hour, 3 hours, 5 hours and 24 hours after administration of the phlogogenic agent.
Results and Discussion. The results of experimental studies indicate that the anti-inflammatory activity of the studied extracts was already manifested at 1 hour of the experiment. At 5 hours of the experiment, the antiexudative activity of the test extracts was maximum. After 24 hours from the onset of inflammation, the effectiveness of the use of extracts of Gentiana asсlepiadea decreased slightly. It was found that a thick extract showed a better anti-inflammatory effect.
Conclusions. Anti-inflammatory activity of of thick and liquid extracts of Gentiana asсlepiadea roots in the model of formalin edema was studied. The test extracts at a dose of 100 mg/kg animal body weight were found to exhibit moderate anti-inflammatory activity.
References
Yakovlyeva, L.V., & Titova O.O. (2019). Study of the assortment of NSAIDs group of drugs on the pharmaceutical market in Ukraine for the period 2014-2018. Pharmaceutical Journal, 4, 41-51 [in Ukrainian].
Vlasova, I.K., Botsula, I.V., Kireyev, I.V., & Koshovyi, O.M. (2023). Study of the chemical composition and anti-inflammatory activity of dry extracts from large-fruited cranberry leaves. Bulletin of Pharmacy, 1 (105), 8-13 [in Ukrainian].
Hladkykh, F.V. (2022). Nonsteroidal anti-inflammatory drugs: therapeutic and undesirable effects, ways of their optimization: monograph. Vinnytsia: TVORY. 216 [in Ukrainian].
Kononenko, G.V., Drohovoz, S.M., & Shchokina, K.G. (2013). Study of the anti-inflammatory activity of a thick extract of mountain ash in a model of adjuvant arthritis in rats. Bulletin of Pharmacy, 1 (7), 70-73 [in Ukrainian].
Gómez-Acebo, I., Dierssen-Sotos, T., Pedro, M. (2018). Epidemiology of non-steroidal anti-inflammatory drugs consumption in Spain. The MCC-Spain study. BMC Public Health, 18, 1134.
Kashuba, O.V. (2015). Adverse reactions caused by the use of non-steroidal anti-inflammatory drugs –analgesics-antipyretics. Ukrainian Medical Journal, 3 (107), 74-76 [in Ukrainian].
Pyda, V.P., Fira, L.S., & Lykhatskyi, P.H. (2018). Study of anti-inflammatory activity of dry extract from lettuce leaves. Medical and Clinical Chemistry, 3 (20), 57-62 [in Ukrainian].
Shatalova, O.M., & Mykhaylenko, O.O. (2019). Experimental study of the anti-inflammatory activity of extracts from plants of the Iridaceae family. Ukrainian Biopharmaceutical Journal, 1 (58), 39-43 [in Ukrainian].
Hrytsyk, R.A, Kireyev, I.V., Struk, O.A., & Klymenko, A.O. (2020). Research of anti-inflammatory activity of artemisia absinthium L. and Artemisia vulgaris L. extracts under conditions of the pathology of different genesis. Medical and Clinical Chemistry, 2 (22), 87-93 [in Ukrainian].
Buza, V., Niculae, M., Hanganu, D., Pall, E., Burtescu, R.F., Olah, N.K., … & Ștefănuț, L.C. (2022). Biological Activities and Chemical Profile of Gentiana asclepiadea and Inula helenium Ethanolic Extracts. Molecules, 27 (11), 3560. https://doi.org/10.3390/molecules27113560
Mihailovic V., Matic S., Mišic D., Solujic S., Stanic S., Katanic J. … & Stankovic N. (2013). Chemical composition, antioxidant and antigenotoxic activities of different fractions of Gentiana asclepiadea L. roots extract. EXCLI J., 12, 807-823. https://www.excli.de/index.php/excli/article/view/1194
Jiang M., Cui B.W., Wu Y.L., Nan J.X., & Lian L.H. (2021). Genus Gentiana: A review on phytochemistry, pharmacology and molecular mechanism. J. Ethnopharmacol, 264, 113391. https://doi.org/10.1016/j.jep.2020.113391
Hrodzinskyy, A.M. (Ed.). (1990). Medicinal plants: Encyclopedic reference. Kyiv: Chairman. ed. URE, 120-121 [in Ukrainian].
Stefanović O., Ličina B., Vasić S., Radojević I., & Čomić L. (2018). Bioactive extracts of Gentiana asclepiadea: Antioxidant, antimicrobial, and antibiofilm activity. Bot. Serbica, 42, 223-229. https://doi.org/10.5281/zenodo.1468319
Mihailović V., Mihailović M., Uskoković A., Arambašić J., Mišić D., Stanković V. … & Matić S. (2013). Hepatoprotective effects of Gentiana asclepiadea L. extracts against carbon tetrachloride induced liver injury in rats. Food Chem. Toxicol, 52, 83-90. https://doi.org/10. 1016/j.fct.2012.10.034
Milutinović M., Dimitrijević-Branković S., & Rajilić-Stojanović M. (2021). Plant Extracts Rich in Polyphenols as Potent Modulators in the Growth of Probiotic and Pathogenic Intestinal Microorganisms. Front. Nutr., 8, 688843. https://doi.org/10.3389/fnut.2021.688843.
Stefanov, O.V. (Ed.). (2001). Preclinical research of medicines. Kyiv: Avicenna [in Ukrainian].
Hrytsyk N. Yu., Erstenyuk H. M. (2022). The study of the hepatoprotective activity of the extracts of Gentiana asclepiadea on the model of acute tetrachloromethane hepatitis. Art of Medicine, 2022, 4 (24), 21-26 [in Ukrainian]. https://doi.org/10.21802/artm.2022.4.24.21
Hrytsyk, N.Yu., Erstenyuk, H.M. (2023). Study of the acute toxicity of extracts of Gentiana asclepiadea. Art of Medicine, 2(26), 29-34 [in Ukrainian]. https://doi.org/10.21802/artm.2023.2.26.29
Law of Ukraine No. 3447-IV of February 21, 2006 “On the Protection of Animals from Cruel Treatment” (as amended). [Electronic resource]. Access mode: https://zakon.rada.gov.ua/laws/show/3447-15#Text
Commission of the European Communities: Council Directive of 18 December 1986 on the Lows, regulating the Application of Principles of Good Laboratory Practice and the Verification of Their Applications for Tests on Chemical Substances (87/18/EEC). (1991). The Rules Governing Medicinal Products in the European Com-munity, 1, 145-146.
Konya R., Reis R., Sipahi H., Barta A., Hohmann J., & Kırmızıbekmez H. (2023). Secondary metabolites from Gentiana cruciata L. and their anti-inflammatory and analgesic activities. Nat Prod Res., 37(18), 3025-3032. https://doi.org/10.1080/14786419.2022.2144301.
Zhang L., Ulriksen E.S., Hoel H., Sandvik L., Malterud K.E., Inngjerdingen K.T. … & Wangensteen H. (2023). Phytochemical characterization and anti-inflammatory activity of a water extract of Gentiana purpurea roots. J Ethnopharmacol, 301, 115818. https://doi.org/10.1016/j.jep.2022.115818
Seiwerth J., Tasiopoulou G., Hoffmann J., Wölfle U., Schwabe K., Quirin K.W., & Schempp C.M. (2019). Anti-Inflammatory Effect of a Novel Topical Herbal Composition (VEL-091604) Consisting of Gentian Root, Licorice Root and Willow Bark Extract. Planta Med., 85(7), 608-614. https://doi.org/10.1055/a-0835-6806
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