FEATURES OF CHANGES IN INDICATORS OF THE ANTIOXIDANT SYSTEM AND STRUCTURE OF THE PROSTATE GLAND AT ETHANOL INTOXICATION
DOI:
https://doi.org/10.11603/mcch.2410-681X.2023.i3.14127Keywords:
prostate, antioxidant system, ethanolAbstract
Introduction. Ethanol intoxication damages almost all organs and systems of the body. Structural and functional changes of the prostate gland have not been fully studied.
The aim of the study – to experimentally investigate changes in the indicators of the antioxidant system and structures of the prostate gland at ethanol intoxication.
Research Methods. Experiments were conducted on 62 sexually mature male rats, which were divided into two groups. Group 1 (30 animals) served as a control, the animals of group 2 (32 rats) were injected intragastrically with a 30 % ethanol solution at a dose of 2 ml per 100 g of body weight for 28 days once a day. A month after the start of the experiment, the experimental animals were euthanized by bloodletting under thiopental anesthesia. The activities of superoxide dismutase, glutathione peroxidase, glutathione reductase, catalase, the content of SH groups and ceruloplasmin were determined in prostate homogenates. Histological preparations were made from the prostate, on which the relative volumes of damaged endotheliocytes of arteries, veins, glandular epitheliocytes, myocytes, and stromal-parenchymatous relations in the examined organ were determined histostereometrically. A correlation analysis was conducted between the studied quantitative morphological parameters and indicators of antioxidant protection with the determination of the pairwise correlation coefficient. Quantitative indicators were processed statistically.
Results and Discussion. Long-term ethanol intoxication of experimental animals leads to a pronounced deterioration of the antioxidant protection of the prostate and damage to its structural components. Correlation analysis revealed strong and significant inverse relationships mainly between enzymatic indicators of antioxidant protection and the relative volume of damaged glandular epitheliocytes, endotheliocytes of arteries, veins, and stromal-parenchymatous relations of the prostate.
Conclusions. Long-term ethanol intoxication of laboratory sexually mature white male rats the antioxidant protection in the prostate significantly deteriorates, which is evidenced by a pronounced decrease in the activity of superoxide dismutase, glutathione peroxidase, glutathione reductase, catalase, the content of SH groups and ceruloplasmin. The expression of violations of the antioxidant protection of the organ under study correlates with the degree of its structural damage.
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