ACTIVITY OF THE ANTIOXIDANT SYSTEM IN DIMETHYLHYDRAZINE INDUCED CARCINOGENESIS AFTER THE APPLICATION OF DRY EXTRACT FROM HOSTA LANCIFOLIA LEAVES
DOI:
https://doi.org/10.11603/mcch.2410-681X.2023.i1.13737Keywords:
Hosta lanifolia, dry extract, 1,2-dimethylhydrazine hydrochloride, colorectal cancer, oxidative stress, oncoprotective actionAbstract
Introduction. Surgery and chemotherapy are the most commonly used forms of treatment for colon cancer due to the lack of scientifically proven alternatives. However, the development and study of new drugs capable of preventing the formation or inhibiting the already existing process of carcinogenesis without causing toxic effects or being toxic to normal cells is extremely important.
The aim of the study – to investigate the indicators of oxidative processes and the antioxidant system in rats with chemically induced colon carcinogenesis against the background of the use of a dry extract from the leaves of hosta lancifolia.
Research Methods. Experimental work was carried out on white male rats. The animals were divided into 3 groups, one of which served as a control. Chronic oncogenic intoxication was simulated by administering 1,2-dimethylhydrazine hydrochloride for 30 weeks (1 time per week). The correction of the toxic lesion was carried out with a dry extract from the leaves of hosta lancifolia, which was administered intragastrically daily at a dose of 100 mg/kg of the animal's body weight throughout the experiment. Liver homogenate and blood serum of rats were taken monthly for research. The activity of oxidizing processes and the state of antioxidant systems were assessed by the content of products of oxidative modification of neutral and basic proteins, superoxide dismutase and catalase activity, the content of reduced glutathione and ceruloplasmin.
Results and Discussion. An increase in the activity of free-radical oxidation processes after damage to rats with 1,2-dimethylhydrazine was proven. This is indicated by a decrease in superoxide dismutase and catalase activity, the content of reduced glutathione, an increase in the content of ceruloplasmin and products of oxidative modification of proteins in the blood serum and liver of animals. It has been experimentally proven that the daily use of a dry extract from hosta lancifolia reliably reduces the content of products of oxidative modification of proteins and normalizes the activity of studied enzymes in the blood serum and liver of animals with an induced oncological process.
Conclusions. The use of a dry extract from hosta lancifolia leaves in the conditions of chemically induced carcinogenesis of colon in rats caused a decrease in the indicators of oxidative modification of proteins and normalization of the indicators of antioxidant protection, which indicates the suppression of oxidative stress in animals under conditions of carcinogen long-term use.
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