DYNAMICS OF INDICATORS OF THE PROOXIDANT-ANTIOXIDANT SYSTEM IN RATS WITH SIMULATING ACUTE GENERALIZED PERITONITIS ASSOCIATED WITH OBESITY
DOI:
https://doi.org/10.11603/mcch.2410-681X.2022.i3.13358Keywords:
acute generalized peritonitis, obesity, antioxidant system, prooxidant systemAbstract
Introduction. The basis of t severe course and high mortality rates in acute generalized peritonitis (AGP) is often a concomitant pathology, among which obesity is present in 7.5 % to 14.0 %.
The aim of the study – to investigate the state of indicators of lipid peroxidation and the antioxidant system in the body of experimental animals with simulated AGP on the background of obesity and to compare them with groups of animals with obesity and with animals with AGP.
Research Methods. In the experiment, 24 white Wistar rats were used, which were divided into three groups: the main group – 8 animals with АGP modeling on the background of obesity; comparison group – 8 animals with obesity simulation; another comparison group – 8 animals with АGP modeling; the control group consisted of 8 intact animals. АGP was modeled by injecting 10 % filtered fecal suspension into the abdominal cavity. Obesity was simulated using a high-calorie diet. The content of indicators of prooxidant-antioxidant protection in the blood and liver of animals was determined using the photospectrometric method.
Results and Discussion. It was established that in animals with АGP against the background of obesity, the indicators of lipoperoxidation were significantly activated (by 1.8 times the level of TBА-AP compared to intact animals) and the antioxidant defense was depleted (according to a statistically significantly lower index of superoxide dismutase by 2.4 times compared to the control)) (p<0.05).
Conclusions. In animals with acute generalized peritonitis, the protective mechanisms of antioxidant protection and the activation of lipid peroxidation processes (according to the level of TBA-AP) significantly decrease on the background of obesity, which is confirmed by statistically significantly lower levels of superoxide dismutase compared to animals with isolated obesity or animals with acute generalized peritonitis.
References
Cullis, B., Al-Hwiesh, A., & Kilonzo, K. (2020). ISPD guidelines for peritoneal dialysis in acute kidney injury: 2020 update (adults). Perit Dial Int., 41 (1), 15-31.
Pörner, D., Von Vietinghoff, S., Nattermann, J., Strassburg, C.P., & Lutz, P. (2021). Advances in the pharmacological management of bacterial peritonitis. Expert Opin. Pharmacother., 22 (12), 1567-1578.
Choua, O., Ali, M.M., Kaboro, M., Moussa, K.M., & Anour, M. (2017). Med Sante Trop. Etiological, clinical, and therapeutic aspects of acute generalized peritonitis in N'Djamena. Chad., 27 (3), 270-273.
Benedict, V.V., Prodan, A.M., & Bagrii, V.M. (2022). Laparostomy in patients with acute disseminated peritonitis. Hospital Surgery. Journal named after L. Ya. Kovalchuk, 2, 42-45 [in Ukrainian].
Verba, R.V., & Klishch, I.M. (2018). Peculiarities of the immune profile in the blood of rats with acute disseminated peritonitis against the background of hypothyroidism. Medical and Clinical Chemistry, 19 (4), 10-17 [in Ukrainian].
Bradshaw, T., & Mairs, Н. (2014). Obesity and serious mental ill health: a critical review of the literature. Healthcare, 2, 166-182.
Sharma, A.M., & Kushner, R.F. (2009). A proposed clinical staging system for obesity. Int. J. Obesity, 33, 289-295.
(2012). Obesity. Situation and trends. Geneva: World Health Organization.
Ovcharuk, V.V., Boychuk, A.V., & Khlibovska, O.I. (2017). Dynamics of indicators of the antioxidant system and lipid peroxidation in pregnant women with placental dysfunction under the influence of complex therapy. Achievements of Clinical and Experimental Medicine, (3), 112-116 [in Ukrainian].
Speakman, J., Hambly, C., Mitchell, S., & Król, E. (2007). Animal models of obesity. Obesity Reviews, 1 (1-2), 55-61.
Lazarenko, V.A., Lipatov, V.A., Blinkov, Yu.Yu., & Skorikov, D.V. (2008). Experimental model of widespread fecal peritonitis. Man and his Health, 4, 128-132 [in Russian].
Togaybayev, A.A., Kurguzkin, A.V., & Rikun, I.V. (1988). [Method for the determination of endogenous intoxication]. Laboratory Work, 9, 22-24 [in Russian].
Shcherbak, O.V., Maslova, G.S., & Skrypnyk, I.M. (2017). Features of the pathogenesis of atherogenic dyslipidemia in patients with coronary heart disease in combination with nonalcoholic steatohepatitis. Bulletin of the Problems of Biology and Medicine, (2), 199-203 [in Ukrainian].
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Medical and Clinical Chemistry
This work is licensed under a Creative Commons Attribution 4.0 International License.
