DEVELOPMENT OF A METHODOLOGY FOR DETERMINING THE QUALITATIVE COMPOSITION OF AMINO ACIDS IN CREEPING THYME LIQUID EXTRACT
Keywords:creeping thyme herb, thyme creeping, liquid extract, identification, thin layer chromatography, amino acids
Introduction. In recent years, the number of studies related to the study of such biologically active substances as amino acids has increased significantly. According to the sources of literature, they show diverse pharmacological activity and thereby perform important functions in the human body. In particular, it has been found that some amino acids exhibit anti-inflammatory, immunomodulatory, soothing activity, etc. Therefore, it is appropriate to consider them as promising BAS for the creation of new medicines. Pharmaceutical development on the basis of thick extract of creeping thyme and thyme essential oil of ordinary provided, first of all, the selection of the optimal method of obtaining the creeping thyme liquid extract (CT), which was obtained using the technology , which allowed in a short time to obtain the maximum extract of BAS and reproduce, accordingly, the qualitative composition of the raw materials under investigation. Since, when standardizing creeping thyme herb, among the main quality markers, amino acids were also chosen, so it is necessary to confirm’ their quality composition in the liquid extract of CT, which is an integral stage in the standardization of the extract under study.
The aim of the study – to develop a methodology for determining the quality composition of amino acids in liquid CT extract and offer appropriate quality markers for its standardization.
Research Мethods. Creeping thyme liquid extract, standard samples: glycine, glutamic acid (Sigma), leucine, tyrosine, alanine, aspartic acid (Fluka), fine-layer chromatography (TLC), Silica gel F254 chromatographic plates of “Merck”, device for automatic application of samples on the plate “CAMAG Linomat 5”, chromatographic camera “GAMAG”, dryer.
Results and Discussion. To standardize creeping thyme liquid extract, it is necessary to monitor the possibility of identifying the main creeping thyme BAS, continuing the proposed approach: creeping thyme herb – liquid extract – thick extract – finished medicinal product. Identification of amino acids in CТ liquid extract was carried out in the solvent system: isopropanol R – formic acid R – water R in the ratio (40:2:10) by pharmacopoeia analysis method – thin-layer chromatography ascending method. To develop this technique, various solvent systems, methods and conditions of chromatography were studied, as a result of which the optimal solvent system with good separating ability was selected.
Conclusions. The method of determining the qualitative composition of amino acids in creeping thyme liquid extract has been developed. As a result of this experiment, six amino acids were identified and appropriate quality markers were proposed for standardization of the extract under study.
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