DYNAMICS OF CHANGES IN BIOCHEMICAL INDICATORS OF BLOOD AND STRUCTURES OF RENAL CORTEX OF WHITE RATS UNDER CONDITIONS OF TOXIC LIVER INJURY
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.v.i2.11362Keywords:
toxic hepatitis, biochemical parameters of blood, kidney, nephron, morphological changesAbstract
Introduction. The high incidence of toxic hepatitis with the development of multiple organ failure determines the relevance of the study of changes in blood biochemical parameters and elucidation of objective morphometric changes in nephron structures that characterize morphofunctional changes in the kidneys in toxic liver damage.
The aim of the study – to learn the dynamics of changes in the biochemical parameters of blood as well as morphological changes in the structures of the cortical substance of the kidneys of white rats under conditions of experimental toxic liver injury.
Research Methods. The experiments were performed on 30 white male rats, which were divided into 3 groups. The model of toxic hepatitis was intoxication with carbon tetrachloride, which was administered intragastrically every other day in the form of 50 % oil solution at a dose of 2 g/kg body weight. Duration of the experiment – 2 and 14 days. The concentrations of total bilirubin, urea, creatinine, aspartate and alanamine aminotransferase activity were determined in blood. For histological examinations, material was taken from the renal cortex, histological preparations were made and stained with hematoxylin and eosin.
Results and Discussion. It was found that in the blood of animals with toxic hepatitis on the 2nd and 14th day of the experiment there was an increase in total bilirubin in 2.4 and 3.2 times. The increase in the activity of intracellular enzymes ALT and AST in 9.5 and 17.4 times and 14.1 and 7.3 times in the corresponding terms of the experiment indicated intense damage of hepatocytes. At the same time, deterioration of the functional state of the kidneys was noted, evidenced by an increase in the concentration of urea in blood by 1.8 and 2.2 times and creatinine by 1.2 and 2.3 times in the respective terms. Histologically in the cortical substance of the kidneys were found signs of hemodynamic disorders, the presence of renal corpuscles with signs of hypertrophy and hypotrophy. Their ratio changed during the experiment. At the same time, there were a narrowing of the lumen of the proximal convoluted tubules, a decrease in the area of their epitheliocytes and the development of dystrophic changes. The corresponding parameters of the distal convoluted tubules underwent less prominent changes.
Conclusion. Toxic liver injury caused damage of the cell membranes of hepatocytes, there was a significant increase in the activity of ALT and AST, total bilirubin. The functional state of the kidneys has deteriorated. At the same time, changes in the structural components of the renal cortex on the background of hemodynamic disorders were found.
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