@article{Yaremchuk_Posokhova_Kuzmak_2020, title={INFLUENCE OF L-ARGININE AND AMINOGUANIDIN ON THE LEVEL OF SYNTHESIS OF NITRIC OXIDE IN THE CEREBELLUM AND CEREBRAL HEMISPHERES IN EXPERIMENTAL ANTIPHOSPHOLIPID SYNDROME}, url={https://ojs.tdmu.edu.ua/index.php/MCC/article/view/10696}, DOI={10.11603/mcch.2410-681X.2019.v.i4.10696}, abstractNote={<p><strong>Introduction.</strong> Antiphospholipid syndrome (APS) is an autoimmune disease characterized by the presence of antiphospholipid antibodies, arterial and venous thrombosis, thrombocytopenia, miscarriage.</p> <p><strong>The aim of the study</strong> – to investigate the effect of the combined effect of L-arginine and aminoguanidine on the content of nitrite anions (NO<sub>2</sub><sup>–</sup>) and nitrate anions (NO<sub>3</sub><sup>–</sup>) in the cerebellum and cerebral hemispheres of BALB/c mice with APS before pregnancy and on the 18<sup>th</sup> day of pregnancy.</p> <p><strong>Research Methods.</strong> The study was performed on female BALB/c mice, for which APS was simulated. L-arginine (25 mg/kg) in combination with aminoguanidine (10 mg/kg) was used for correction. The content of NO in the homogenates of cerebellum and cerebral hemispheres of the animals with APS before pregnancy and on the 18<sup>th</sup> day of pregnancy was determined by the number of its stable metabolites of nitrite anions (NO<sub>2</sub><sup>–</sup>) and nitrate anions (NO<sub>3</sub><sup>–</sup>).</p> <p><strong>Results and Discussion.</strong> The content of stable metabolites of nitric oxide NO<sub>2</sub><sup>–</sup> and NO<sub>3</sub><sup>–</sup> in the cerebellum and cerebral hemispheres increased in the BALB/c mice with APS compare to the intact animals. In cases of experimental APS, on the 18<sup>th</sup> day of pregnancy, an increase in the content of NO<sub>2</sub><sup>–</sup> and NO<sub>3</sub><sup>–</sup> in the cerebellum was evidenced, as well as a decrease in NO<sub>2</sub><sup>–</sup> and NO<sub>3</sub><sup>–</sup> in the brain hemispheres of the animals with APS compare to the controls. In case of the combined administration of precursor of synthesis of NO L-arginine and a selective inhibitor of iNOS aminoguanidine into the animals with APS, a decrease in the content of NO<sub>2</sub><sup>–</sup> and NO<sub>3</sub><sup>–</sup> in the cerebellum (by 28 % and 17 %) and cerebral hemispheres (by 16 % and 10 %) was evidenced comparing to the animals with APS. In the pregnant BALB/c mice with APS administered with L-arginine in combination with aminoguanidine, a decrease of NO<sub>2</sub><sup>–</sup> by 29 % and NO<sub>3</sub><sup>–</sup> by 25 % in cerebellum, an increase of NO<sub>2</sub><sup>–</sup> by 21 % and NO<sub>3</sub><sup>–</sup>by 22 % in the cerebral hemispheres was evidenced comparing to those of the pregnant females with APS.</p> <p><strong>Conclusions.</strong> It has been established that the combined use of the precursor of synthesis of NO L-arginine and a selective inhibitor iNOS aminoguanidine leads to normalization of the level of stable metabolites of NO<sub>2</sub><sup>–</sup> and NO<sub>3</sub><sup>–</sup> nitric oxide synthesis in the cerebellum and cerebral hemispheres of the BALB/c mice with APS before pregnancy and on the 18<sup>th</sup> day of pregnancy.</p>}, number={4}, journal={Medical and Clinical Chemistry}, author={Yaremchuk, O. Z. and Posokhova, K. A. and Kuzmak, I. P.}, year={2020}, month={Jan.}, pages={105–112} }